Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,803,230	C→A	R15S (CGC→AGC)	dnaB →	replicative DNA helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,803,230	0	C	A	100.0%	340.8 / NA	91	R15S (CGC→AGC)	dnaB	replicative DNA helicase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (0/91);  total (0/91)

GCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAA  >  minE/2803191‑2803261                                        |                                gCAGTAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:620960/71‑1 (MQ=255) gCAGGAAATAAACGCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:261852/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:392536/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:546420/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:543292/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:511793/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:50415/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:499900/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:476054/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:455190/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:421330/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:409920/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:236495/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:360727/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:358916/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:35087/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:347998/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:314947/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:31252/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:307382/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:268803/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:998929/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:812842/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1002061/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:980793/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:969020/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:95090/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:902934/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:87473/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:86967/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:868969/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:84439/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:81538/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:552695/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:777504/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:755199/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:754657/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:736559/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:735313/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:692617/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:690161/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:650581/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:644639/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1257915/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1344650/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1341944/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1328394/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1327150/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1315398/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:131470/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1310566/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1301550/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1284810/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:126936/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:162304/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1219761/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1182665/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:112490/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1083067/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1073201/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1052903/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1042482/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1033310/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1004573/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1003872/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1470941/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:174971/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1521603/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1504860/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1502938/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1480440/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1474323/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1364758/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1468533/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1463695/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1457856/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1447667/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1424290/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1424163/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1409348/71‑1 (MQ=255) gCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1369850/71‑1 (MQ=255) gCAGGAAATAAACCCTACAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1491381/71‑1 (MQ=255) gCAGAAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:997124/71‑1 (MQ=255)  cAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1161800/70‑1 (MQ=255)  cAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1533002/70‑1 (MQ=255)      aaaTAAACCCTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:570014/66‑1 (MQ=255)              ccTTCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:609660/58‑1 (MQ=255)                ttCAACAAACAGCAGGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:327457/56‑1 (MQ=255)                           gcagGCTGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1384526/45‑1 (MQ=255)                                 tGAACCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:1106338/39‑1 (MQ=255)                                    aCCCAGCGAACGCGATCCACAAGTTGCCGGGCTGaa  <  1:472650/36‑1 (MQ=255)                                        |                                GCAGGAAATAAACCCTTCAACAAACAGCAGGCTGAACCCCGCGAACGCGATCCACAAGTTGCCGGGCTGAA  >  minE/2803191‑2803261

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑