| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,847,805 | G→T | F1076L (TTC→TTA) | yjeP ← | predicted mechanosensitive channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,847,805 | 0 | G | T | 79.1% | 387.0 / 133.3 | 191 | F1076L (TTC→TTA) | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base G (0/40); new base T (151/0); total (151/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.81e-42 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01 | |||||||||||
Only 100 of 191 total aligned reads displayed.
GTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATC > minE/2847746‑2847859 | gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:495644/71‑1 (MQ=255)gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:1478106/71‑1 (MQ=255)gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:638161/71‑1 (MQ=255)gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:560510/71‑1 (MQ=255)gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:1272158/71‑1 (MQ=255)gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:1245946/71‑1 (MQ=255)gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:819257/71‑1 (MQ=255)gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:117860/71‑1 (MQ=255)gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:86871/71‑1 (MQ=255)gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:1016148/71‑1 (MQ=255)gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:110029/71‑1 (MQ=255)gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:1072365/71‑1 (MQ=255)gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:428823/71‑1 (MQ=255)gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:1028020/71‑1 (MQ=255)gTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:981557/71‑1 (MQ=255)gTCAGCGTTCTCCCCGTTTGATTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:1412197/71‑1 (MQ=255) cAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:785928/69‑1 (MQ=255) ttCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:327658/64‑1 (MQ=255) cGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:837921/57‑1 (MQ=255) ttACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:454717/50‑1 (MQ=255) cGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATc < 1:1229802/46‑1 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGt > 1:911966/1‑36 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCtgttgg > 1:816211/1‑68 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCtgg > 1:1421630/1‑65 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCt > 1:932105/1‑63 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGa > 1:1530904/1‑69 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:52872/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:554475/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:526377/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:561505/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:565258/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:578045/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:583872/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:995373/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:587739/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:407572/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:484442/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:466750/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:750715/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1009331/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:971466/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:948162/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:938842/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:920262/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:840075/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:810911/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:770483/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:60220/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:724104/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:714443/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:702228/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:671907/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:656304/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:646943/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:628118/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:611947/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:11884/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:137151/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:391458/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1342229/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1334024/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1312093/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1284745/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1252655/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:122640/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1200792/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1194020/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1360263/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1171012/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1166558/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1158272/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1152864/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1127475/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1082053/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1041086/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1038455/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1023467/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:221390/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:385334/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:357163/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:337798/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:305882/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:300343/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:274410/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:255261/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:244285/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:222554/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1376924/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:205448/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:191958/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1551071/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1429565/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1412133/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:1400885/1‑71 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAt > 1:1206897/1‑70 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAt > 1:1154914/1‑70 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAt > 1:161781/1‑70 (MQ=255) gCATCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAt > 1:1507039/1‑70 (MQ=255) gCACCTGGAAGGGCGGTAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGAtc > 1:228407/1‑71 (MQ=255) gCAACTGGAAGGGCGGTAATGGCATATCGATACCGTgg > 1:804921/1‑38 (MQ=38) | GTCAGCGTTCTCCCCGTTTGTTTACCGTTGAGGCTTTCCAGACGCATCTGGAAGGGCGGGAATGGCATATCGATACCGTGGGCATGGAAGCCAGCCAGAATCAGCTGGTGGATC > minE/2847746‑2847859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |