| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 197,146 | 3 bp→CCG | coding (453‑455/1719 nt) | proS ← | prolyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 197,146 | 0 | G | C | 100.0% | 26.9 / NA | 9 | S152G (TCC→GGC) | proS | prolyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (9/0); total (9/0) | |||||||||||
| * | minE | 197,147 | 0 | A | C | 100.0% | 26.9 / NA | 9 | S152G (TCC→GGC) | proS | prolyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (9/0); total (9/0) | |||||||||||
| * | minE | 197,148 | 0 | A | G | 100.0% | 25.6 / NA | 9 | R151R (CGT→CGC) | proS | prolyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (9/0); total (9/0) | |||||||||||
TCATCAGGAATTCGCGGGAACGCATGACGCCGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTG > minE/197129‑197195 ||| tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCt > 1:674107/1‑66 (MQ=255)tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg > 1:1095652/1‑67 (MQ=255)tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg > 1:1124433/1‑67 (MQ=255)tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg > 1:1158123/1‑67 (MQ=255)tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg > 1:1478825/1‑67 (MQ=255)tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg > 1:537409/1‑67 (MQ=255)tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg > 1:542404/1‑67 (MQ=255)tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg > 1:588324/1‑67 (MQ=255)tcatcgTGAATTCGCGGCCGCGCATCAGGCCGAAGCGCGGACGGCGCTCGTCGCGGAACTTGGTCTg > 1:682912/1‑67 (MQ=255) ||| TCATCAGGAATTCGCGGGAACGCATGACGCCGAAACGCGGACGCACTTCGTCGCGGAACTTGGTCTG > minE/197129‑197195 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |