Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,955,451	2 bp→CT	coding (516‑517/678 nt)	yjjG →	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,955,451	0	G	C	100.0%	41.1 / NA	13	L172L (CTG→CTC)	yjjG	predicted hydrolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (0/13);  total (0/13)
*	minE	2,955,452	0	A	T	100.0%	42.1 / NA	13	M173L (ATG→TTG)	yjjG	predicted hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (0/13);  total (0/13)

TTCACGCGTGCTGATGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCG  >  minE/2955439‑2955508             ||                                                         cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg  <  1:1010532/67‑1 (MQ=255) cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg  <  1:1081837/67‑1 (MQ=255) cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg  <  1:1148523/67‑1 (MQ=255) cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg  <  1:1169991/67‑1 (MQ=255) cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg  <  1:126170/67‑1 (MQ=255) cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg  <  1:1482920/67‑1 (MQ=255) cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg  <  1:1485783/67‑1 (MQ=255) cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg  <  1:256844/67‑1 (MQ=255) cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg  <  1:31298/67‑1 (MQ=255) cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg  <  1:411690/67‑1 (MQ=255) cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg  <  1:717887/67‑1 (MQ=255) cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg  <  1:913216/67‑1 (MQ=255) cagaCGTGTGCTCTTGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCg  <  1:997480/67‑1 (MQ=255)             ||                                                         TTCACGCGTGCTGATGGTTGGCGACACTGCCGAGTCCGATATTCTCGGTGGCATCAACGCCGGGCTTGCG  >  minE/2955439‑2955508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑