| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,196,798 | (C)6→7 | coding (354/1911 nt) | yoaA ← | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,196,792 | 1 | . | C | 97.6% | 137.4 / ‑3.4 | 41 | coding (360/1911 nt) | yoaA | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (14/26); total (15/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01 | |||||||||||
CGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCG‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCA > minE/1196728‑1196856 | cGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCG‑ccccc > 1:1133897/1‑70 (MQ=255) tGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAg > 1:280069/1‑69 (MQ=255) gATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAgcg < 1:2894350/70‑1 (MQ=255) aTTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAgcgc < 1:2497679/70‑1 (MQ=255) aTTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAgcgc < 1:246532/70‑1 (MQ=255) aTTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGcc > 1:860083/1‑71 (MQ=255) tAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCct > 1:691811/1‑70 (MQ=255) ccAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGt < 1:635875/71‑1 (MQ=255) ccAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGt < 1:2101789/71‑1 (MQ=255) cAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGtt > 1:995008/1‑71 (MQ=255) agcagGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGtt < 1:2228102/71‑1 (MQ=255) gcagGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTc < 1:1702667/71‑1 (MQ=255) cagGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTc > 1:742330/1‑70 (MQ=255) cagGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCg < 1:622037/71‑1 (MQ=255) cagGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCg < 1:510941/71‑1 (MQ=255) cagGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCg < 1:1408542/71‑1 (MQ=255) agGATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGa < 1:3018187/71‑1 (MQ=255) gATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGtt < 1:835455/66‑1 (MQ=255) gATCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAgg < 1:1818669/71‑1 (MQ=255) tCACATCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGATGTTCGAGACGTTCGAgg > 1:2896129/1‑69 (MQ=255) cacaTCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGa > 1:1988026/1‑66 (MQ=255) acaTCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCaga < 1:1700633/71‑1 (MQ=255) acaTCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCag < 1:1085535/70‑1 (MQ=255) acaTCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCag < 1:654933/70‑1 (MQ=255) caTCGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCaga < 1:569806/70‑1 (MQ=255) cGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGtt > 1:2203199/1‑58 (MQ=255) cGCTTAAGATTTGTACCGGCAGATCGCCCCCCCGCAAGCGCCTGCTGTTCGAGACGtt > 1:936606/1‑58 (MQ=255) gCTTAAGATTTGTACCGGCAGATC‑‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGtt > 1:843811/1‑71 (MQ=255) cTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCg < 1:1085958/49‑1 (MQ=38) cTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCg < 1:392662/49‑1 (MQ=38) cTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCg < 1:1730588/49‑1 (MQ=38) cTTAAGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGt < 1:933780/71‑1 (MQ=255) aaGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCGGAGGTAGtt < 1:804436/69‑1 (MQ=255) aaGATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGttt > 1:2477214/1‑70 (MQ=255) gATTTGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAg > 1:645321/1‑71 (MQ=255) tGTACCGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCaga > 1:1695622/1‑56 (MQ=255) ccGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCtt < 1:1260691/70‑1 (MQ=255) ccGGCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCtt < 1:1641591/70‑1 (MQ=255) ggCAGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCagag > 1:1729858/1‑51 (MQ=39) aGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTcagca < 1:341548/71‑1 (MQ=255) aGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTcagca < 1:17428/71‑1 (MQ=255) aGATCGCCCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTcag > 1:1316456/1‑69 (MQ=255) | CGACTGTTTGATTAGACCAGGAGCGCAGCAGGATCACATCGCTTAAGATTTGTACCGGCAGATCG‑CCCCCCGCCAGCGCCTGCTGTTCGAGACGTTCGAGGCAGAGGTAGTTTGAGCGCCCTTTCAGCA > minE/1196728‑1196856 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |