| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,359,867 | 0 | G | A | 36.0% | 140.2 / 116.7 | 111 | noncoding (1228/2905 nt) | rrlA | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base G (40/31); new base A (22/18); total (62/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.50e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 112 total aligned reads displayed.
CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCA > minE/2359815‑2359936 | cccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTca > 1:468243/1‑71 (MQ=31)cccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca < 1:1324283/71‑1 (MQ=9) ccGCTTTATTGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTcac < 1:932242/71‑1 (MQ=11) ccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca > 1:454580/1‑70 (MQ=255) cGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcac < 1:2683683/70‑1 (MQ=33) cGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTca > 1:1518779/1‑69 (MQ=31) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcaca > 1:91801/1‑70 (MQ=35) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAg > 1:1702921/1‑71 (MQ=34) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAg > 1:1281359/1‑71 (MQ=255) cTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAgg < 1:1609711/71‑1 (MQ=34) cTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAgg > 1:40410/1‑71 (MQ=255) ttATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTcaca > 1:1527158/1‑67 (MQ=255) ttATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAg < 1:599684/68‑1 (MQ=255) ttATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt > 1:599191/1‑71 (MQ=255) tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt > 1:1852285/1‑70 (MQ=35) tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt < 1:1722944/70‑1 (MQ=35) tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt < 1:1136251/70‑1 (MQ=35) tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCt < 1:924513/70‑1 (MQ=35) tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGc < 1:1714747/69‑1 (MQ=255) cGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa > 1:1334513/1‑69 (MQ=35) gTTACTTATGTGAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAg < 1:1897609/71‑1 (MQ=21) gTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAg < 1:2845437/71‑1 (MQ=255) ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTa > 1:2229914/1‑67 (MQ=255) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa < 1:1137681/70‑1 (MQ=35) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAAc > 1:1559628/1‑71 (MQ=34) ttATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcaca < 1:249271/57‑1 (MQ=35) tATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCt > 1:2144836/1‑71 (MQ=34) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc < 1:2254526/69‑1 (MQ=35) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc > 1:681108/1‑71 (MQ=34) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAgg < 1:1238677/57‑1 (MQ=11) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCt < 1:2579103/70‑1 (MQ=255) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc > 1:1817243/1‑71 (MQ=255) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc > 1:641149/1‑71 (MQ=255) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc > 1:2752915/1‑71 (MQ=255) tGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc < 1:94313/70‑1 (MQ=35) tGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc < 1:2876350/70‑1 (MQ=35) gTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGc > 1:981149/1‑56 (MQ=35) gTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGc > 1:1837436/1‑56 (MQ=35) gTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTccc > 1:1371916/1‑71 (MQ=34) gTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTccc > 1:2045665/1‑71 (MQ=34) tCAGCATTCGCATTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAg < 1:3017138/53‑1 (MQ=11) tCAGCATTCGCAATTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcccc < 1:419602/71‑1 (MQ=11) cAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcccc > 1:2404638/1‑70 (MQ=35) aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTa > 1:1045999/1‑71 (MQ=255) gCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAc > 1:423270/1‑71 (MQ=34) gCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAgg > 1:626808/1‑51 (MQ=11) gCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAgg > 1:2718194/1‑51 (MQ=11) gCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAc > 1:2437436/1‑71 (MQ=255) cATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAcc > 1:1385380/1‑71 (MQ=34) aTTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAccc < 1:2526081/71‑1 (MQ=255) ttCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAccc > 1:386289/1‑70 (MQ=35) ttCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTcac < 1:612194/45‑1 (MQ=11) tCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:2774872/1‑71 (MQ=34) cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:901703/1‑70 (MQ=35) cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGaa > 1:2140184/1‑55 (MQ=11) gCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCtt < 1:2493933/48‑1 (MQ=38) gCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac < 1:1843755/70‑1 (MQ=35) gCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac < 1:1516573/70‑1 (MQ=255) aCTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa > 1:1589510/1‑70 (MQ=35) cTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca < 1:1295222/71‑1 (MQ=35) ttCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCtt < 1:1420924/44‑1 (MQ=38) ttCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca > 1:292532/1‑70 (MQ=37) ttCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca > 1:2676879/1‑70 (MQ=37) ttCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg > 1:2050316/1‑69 (MQ=11) ttCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaac < 1:2840550/69‑1 (MQ=255) ttCAGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca > 1:332221/1‑70 (MQ=255) tCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacgc > 1:179919/1‑68 (MQ=11) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa > 1:879987/1‑66 (MQ=35) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca > 1:1056932/1‑70 (MQ=40) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca > 1:2788665/1‑70 (MQ=40) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca < 1:176967/70‑1 (MQ=40) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca < 1:2234703/70‑1 (MQ=40) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca < 1:2417745/70‑1 (MQ=40) cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTAc > 1:2510343/1‑44 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacgc < 1:1025730/69‑3 (MQ=11) aTACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATa < 1:2077206/69‑1 (MQ=40) aTACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAg < 1:1676100/70‑1 (MQ=255) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg < 1:1000917/71‑1 (MQ=255) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg < 1:129631/71‑1 (MQ=255) aCCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtgt > 1:1062658/1‑71 (MQ=255) aCCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtgt > 1:429041/1‑71 (MQ=255) tCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCt < 1:2888680/49‑1 (MQ=11) ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt > 1:1710721/1‑71 (MQ=255) ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt > 1:138605/1‑71 (MQ=255) ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt > 1:1760164/1‑71 (MQ=255) ccAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACg > 1:666532/1‑41 (MQ=14) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGc < 1:10336/69‑1 (MQ=255) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTg > 1:2377268/1‑71 (MQ=255) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTg > 1:1317944/1‑71 (MQ=255) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTg > 1:84631/1‑71 (MQ=255) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTg > 1:2831639/1‑71 (MQ=255) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTg > 1:2881610/1‑71 (MQ=255) cAGCATACCTCACAGTACACCTTCACAGGCTTACAGaa > 1:2674938/1‑38 (MQ=14) gCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGcc > 1:1873792/1‑71 (MQ=255) gCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacgc < 1:1248540/57‑3 (MQ=11) aTGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcccc < 1:525678/42‑1 (MQ=38) aTGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACACCACATAGTGTCGCTGCCGc < 1:391602/71‑1 (MQ=255) aTGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGc < 1:2710536/71‑1 (MQ=255) tGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCa < 1:1258185/71‑1 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCg > 1:1088447/1‑68 (MQ=255) | CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCA > minE/2359815‑2359936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |