| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,359,876 | 0 | C | T | 30.8% | 215.0 / 112.8 | 130 | noncoding (1219/2905 nt) | rrlA | 23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base C (51/39); new base T (22/18); total (73/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 133 total aligned reads displayed.
CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTG > minE/2359815‑2359945 | cccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTca > 1:468243/1‑71 (MQ=31)cccGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTca < 1:1324283/71‑1 (MQ=9) ccGCTTTATTGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTcac < 1:932242/71‑1 (MQ=11) cGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcac < 1:2683683/70‑1 (MQ=33) cGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTca > 1:1518779/1‑69 (MQ=31) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcaca > 1:91801/1‑70 (MQ=35) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAg > 1:1702921/1‑71 (MQ=34) gCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAg > 1:1281359/1‑71 (MQ=255) cTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAgg < 1:1609711/71‑1 (MQ=34) cTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAgg > 1:40410/1‑71 (MQ=255) ttATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAg < 1:599684/68‑1 (MQ=255) ttATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCt > 1:599191/1‑71 (MQ=255) tATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGc < 1:1714747/69‑1 (MQ=255) cGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTa > 1:1334513/1‑69 (MQ=35) ttACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTa > 1:2229914/1‑67 (MQ=255) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGaa < 1:1137681/70‑1 (MQ=35) aCTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAAc > 1:1559628/1‑71 (MQ=34) ttATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTcaca < 1:249271/57‑1 (MQ=35) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGc < 1:2254526/69‑1 (MQ=35) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc > 1:681108/1‑71 (MQ=34) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACATTCACAGGCTTACAGAACGCt < 1:1543487/70‑1 (MQ=25) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAgg < 1:1238677/57‑1 (MQ=11) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGc > 1:819019/1‑58 (MQ=11) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCt < 1:2579103/70‑1 (MQ=255) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc > 1:2752915/1‑71 (MQ=255) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc > 1:641149/1‑71 (MQ=255) aTGTCAGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTc > 1:1817243/1‑71 (MQ=255) tGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc < 1:2876350/70‑1 (MQ=35) tGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTc < 1:94313/70‑1 (MQ=35) gTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGc > 1:1837436/1‑56 (MQ=35) gTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGc > 1:981149/1‑56 (MQ=35) gTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGc > 1:1200284/1‑56 (MQ=35) gTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTccc > 1:1371916/1‑71 (MQ=34) gTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTccc > 1:2045665/1‑71 (MQ=34) tCAGCATTCGCATTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAg < 1:3017138/53‑1 (MQ=11) tCAGCATTCGCAATTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTcccc < 1:419602/71‑1 (MQ=11) cAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTcccc > 1:2404638/1‑70 (MQ=35) aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCt < 1:519512/70‑1 (MQ=255) aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTa < 1:1768477/71‑1 (MQ=255) aGCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTa > 1:1045999/1‑71 (MQ=255) gCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAc < 1:1952074/71‑1 (MQ=34) gCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAc > 1:423270/1‑71 (MQ=34) gCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAgg > 1:2718194/1‑51 (MQ=11) gCATTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAc > 1:2437436/1‑71 (MQ=255) cATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTAcc > 1:1385380/1‑71 (MQ=34) aTTCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAccc < 1:2526081/71‑1 (MQ=255) ttCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTcac < 1:612194/45‑1 (MQ=11) tCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:2774872/1‑71 (MQ=34) tCGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTAc > 1:303587/1‑67 (MQ=255) cGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaa > 1:901703/1‑70 (MQ=35) cGCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGaa > 1:2140184/1‑55 (MQ=11) gCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac < 1:1843755/70‑1 (MQ=35) gCACTTCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac < 1:1516573/70‑1 (MQ=255) ttCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCtt < 1:1420924/44‑1 (MQ=38) ttCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca > 1:2676879/1‑70 (MQ=37) ttCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacg > 1:2809478/1‑69 (MQ=11) ttCTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaac < 1:2840550/69‑1 (MQ=255) ttCAGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAacaaca > 1:332221/1‑70 (MQ=255) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa > 1:879987/1‑66 (MQ=35) cTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAAcaca < 1:176967/70‑1 (MQ=40) cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTAc > 1:2510343/1‑44 (MQ=11) cTGATACCTCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacgc < 1:1025730/69‑3 (MQ=11) aTACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATa < 1:2077206/69‑1 (MQ=40) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg < 1:1000917/71‑1 (MQ=255) tACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtg < 1:129631/71‑1 (MQ=255) aCCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtgt > 1:429041/1‑71 (MQ=255) aCCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAgtgt > 1:1062658/1‑71 (MQ=255) tCCAGCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCt < 1:2888680/49‑1 (MQ=11) ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt > 1:1710721/1‑71 (MQ=255) ccAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCt > 1:1760164/1‑71 (MQ=255) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGc < 1:10336/69‑1 (MQ=255) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTg > 1:1317944/1‑71 (MQ=255) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTg > 1:2831639/1‑71 (MQ=255) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTg > 1:2881610/1‑71 (MQ=255) cAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTg > 1:2377268/1‑71 (MQ=255) cAGCATACCTCACAGTACACCTTCACAGGCTTACAGaa > 1:2674938/1‑38 (MQ=14) gCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGcc > 1:1873792/1‑71 (MQ=255) gCATACCTCACAGTACACCTTCACAGGCTTACAGAACGCTCCCCTACCCaacaacgc < 1:1248540/57‑3 (MQ=11) aTGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACACCACATAGTGTCGCTGCCGc < 1:391602/71‑1 (MQ=255) tGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCa < 1:1258185/71‑1 (MQ=255) gCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCg > 1:1088447/1‑68 (MQ=255) ccTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAg < 1:2473044/70‑1 (MQ=255) ccTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGc < 1:389553/71‑1 (MQ=255) ccTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGc < 1:215422/71‑1 (MQ=255) cTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGc < 1:1895328/70‑1 (MQ=255) cTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCt < 1:1105654/71‑1 (MQ=255) tCACAGCACACCTTCACAGGCTTACAGAACGCTcccc < 1:2042361/37‑1 (MQ=40) tCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCcaac > 1:1528304/1‑45 (MQ=38) cacaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTc > 1:645356/1‑71 (MQ=255) cacaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTc > 1:2319380/1‑71 (MQ=255) acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAg > 1:502592/1‑52 (MQ=255) acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCtt < 1:2187130/69‑1 (MQ=255) acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCg > 1:64048/1‑71 (MQ=255) acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCg > 1:2278388/1‑71 (MQ=255) acaGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCg > 1:1668280/1‑71 (MQ=255) caGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCgg > 1:2086814/1‑71 (MQ=255) aGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGc > 1:1897272/1‑62 (MQ=255) gCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTg < 1:776984/71‑1 (MQ=255) cacaCCTTCACAGGCTTACAGAACGCTCCCCTACCcaacaa > 1:1625743/1‑41 (MQ=38) cacaCCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGc < 1:2237283/60‑1 (MQ=255) | CCCGCTTTATCGTTACTTATGTCAGCATTCGCACTTCTGATACCTCCAGCATGCCTCACAGCACACCTTCACAGGCTTACAGAACGCTCCCCTACCCAACAACACATAGTGTCGCTGCCGCAGCTTCGGTG > minE/2359815‑2359945 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |