| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,394,574 | 0 | T | G | 20.4% | 90.6 / 10.1 | 54 | F47C (TTT→TGT) | rarD | predicted chloramphenical resistance permease |
| Reads supporting (aligned to +/- strand): ref base T (11/32); new base G (11/0); total (22/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.37e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTACTTCAAGTTGATTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCTGATGAGCATTTGCCGCCAGTGGTCCTATTTAAAAACGCTGATTCAGACGCCA > minE/2394508‑2394644 | cGTACTTCAAGTTGATTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGttttta > 1:1057011/1‑70 (MQ=255)cGTACTTCAAGTTGATTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTc > 1:207094/1‑71 (MQ=255) tACTTCAAGTTGATTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCtt < 1:369079/71‑1 (MQ=255) tACTTCAAGTTGATTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCtt < 1:497377/71‑1 (MQ=255) tCAAGTTGATTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCttt > 1:1878399/1‑68 (MQ=255) cAAGTTGATTTACTACGTGCCGGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGttt > 1:2159165/1‑61 (MQ=255) cAAGTTGATTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGttt > 1:2190004/1‑61 (MQ=255) cAAGTTGATTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTAtgg > 1:132899/1‑71 (MQ=255) aaGTTGATTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTAtgg < 1:2635648/70‑1 (MQ=255) aGTTGATTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGtttttat > 1:53621/1‑61 (MQ=255) gATTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTtttattta > 1:897896/1‑62 (MQ=255) gATTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTTTggtggt > 1:2995715/1‑69 (MQ=255) aTTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTc > 1:2755490/1‑57 (MQ=255) aTTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCt < 1:2420379/71‑1 (MQ=255) aTTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCt < 1:340716/71‑1 (MQ=255) aTTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCt < 1:2933564/71‑1 (MQ=255) aTTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCt < 1:1226892/71‑1 (MQ=255) tttACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCtg < 1:749518/71‑1 (MQ=255) tttACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCtg < 1:753182/71‑1 (MQ=255) ttACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGtgtttat > 1:1392518/1‑54 (MQ=255) ttACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTAtggtg > 1:38884/1‑64 (MQ=255) tactacGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCtg < 1:815333/69‑1 (MQ=255) actacGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGtttt > 1:114801/1‑51 (MQ=255) acGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTAtg > 1:1052286/1‑56 (MQ=255) acGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTGTTTCTTTAtg > 1:2734838/1‑56 (MQ=255) gTGCCCGCCGATGAAATCCTGACGCTTCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCTGATGAGCa < 1:1417921/70‑1 (MQ=255) tGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCt < 1:351704/48‑1 (MQ=255) tGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCt < 1:1278755/48‑1 (MQ=255) tGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCTGATGAGCAtt < 1:1156068/71‑1 (MQ=255) cccGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCTGATGAGCAttt < 1:2771732/70‑1 (MQ=255) cccGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCTGATGAGCATTTg < 1:1432380/71‑1 (MQ=255) cgccgATGAAATCCTGACGCATCGCGTGATCTGGTCGtttt < 1:636252/41‑1 (MQ=255) cgccgATGAAATCCTGACGCATCGCGTGATCTGGTCGtttt < 1:758963/41‑1 (MQ=255) cgccgATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCTGATGAGCAttt < 1:845793/68‑1 (MQ=255) gccgATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCt < 1:3003018/43‑1 (MQ=255) gccgATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATTGTGGTGCTGATGAGCATTTgcc > 1:1333507/1‑70 (MQ=255) ccgATGAAATCCTGACGCATCGCGTGATCTGGTCGtttttg > 1:2177249/1‑40 (MQ=255) ccgATGAAATCCTGACGCATCGCGTGATCTGGTCGtttt > 1:2793533/1‑39 (MQ=255) ccgATGAAATCCTGACGCATCGCGTGATCTGGTCGTGTTTCTTTa > 1:110636/1‑45 (MQ=255) cgATGAAATCCTGACGCATCGCGTGATCTGGTCGtttt < 1:771776/38‑1 (MQ=255) cgATGAAATCCTGACGCATCGCGTGATCTGGTCGTGTTTCtt > 1:1478608/1‑42 (MQ=255) gATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCt < 1:2453359/53‑1 (MQ=255) gATGAAATCCTGACGCATCGCGTGATCTGGTCGTGTTTCTTTAtg > 1:1144792/1‑45 (MQ=255) aTGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTAtgg < 1:2993927/45‑1 (MQ=255) tGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCTGATGAGCATTTGCCGCCAGt < 1:376636/71‑1 (MQ=255) gAAATCCTGACGCATCGCGTGATCTGGTCGTGTTTGTTTTTggtggt > 1:899679/1‑47 (MQ=255) aaaTCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCtgat < 1:577503/52‑1 (MQ=255) aaaTCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCtgat < 1:2529863/52‑1 (MQ=255) aaaTCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCTGATGAGCATTTGCCGCCAGTgg < 1:527368/71‑1 (MQ=255) aaaTCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCTGATGAGCATTTGCCGCCAGTg < 1:1220247/70‑1 (MQ=255) aaaTCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCTGATGAGCATTTGCCGCCAGTg < 1:172436/70‑1 (MQ=255) aaaTCCTGACGCATCGCGTGATCTGGTCGTGTTTGTTTAttgtg > 1:2984706/1‑44 (MQ=255) aaaTCCTGACGCATCGCGTGATCTGGGCGTTTTTCTTTATGGTGGTGCtgat < 1:1467696/52‑1 (MQ=255) aaaTCCTGACGCATCGCGT‑ATCTGGTCGTGTTTGTTTAtggtgg > 1:510961/1‑44 (MQ=255) aaTCCTGACGCATCGCGTGATCTGGTCGTGTTTGTTTAttgtgg > 1:2281095/1‑44 (MQ=255) aTCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATggtggt > 1:1488161/1‑44 (MQ=255) aTCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCtgat < 1:154431/50‑1 (MQ=255) tCCTGACGCATCGCGTGATCTGGTCGTGTTTGTTTAtggtgg > 1:2829794/1‑42 (MQ=255) ccTGACGCATCGCGTGATCTGGTCGTGTTTCTTTATggtggt > 1:1724237/1‑42 (MQ=255) cTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCt > 1:1970609/1‑44 (MQ=255) aCGCATCGCGTGATCTGGTCGTGTTTGTTTTTGgtggtg > 1:1706970/1‑39 (MQ=37) aTCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGc < 1:419762/36‑1 (MQ=255) tGATCTGGTCGTTTTTCTTTATGGTGGTGCTGATGAGCATTTGCCGCCAGTGGTCCTATTTAAAAACGCTg < 1:2986752/71‑1 (MQ=255) cTGGTCGTTTTTCTTTATGGTGGTGCTGATGAGCATTTgccgcctg < 1:247793/46‑3 (MQ=255) gTCGTTTTTCTTTATGGTGGTGCTGATGAGCATTTGCCGCCAGTGGTCCTATTTAAAAACGCTGAtt < 1:2010170/67‑1 (MQ=255) ttttCTTTATGGTGGTGCTGATGAGCATTTGCCGCCAGTGGTCCTATTTaaa < 1:764333/52‑1 (MQ=255) ttttCTTTATGGTGGTGCTGATGAGCATTTGCCGCCAGTGGTCCTATTTaaa < 1:2083944/52‑1 (MQ=255) ttttCTTTATGGTGGTGCTGATGAGCATTTGCCGCCAGTGGTCCTATTTAAAAACGCTGAt < 1:201038/61‑1 (MQ=255) ttttCTTTATGGTGGTGCTGATGAGCATTTGCCGCCAGTGGTCCTATTTAAAAACGCTGATTCAGACGCca > 1:894940/1‑71 (MQ=255) | CGTACTTCAAGTTGATTTACTACGTGCCCGCCGATGAAATCCTGACGCATCGCGTGATCTGGTCGTTTTTCTTTATGGTGGTGCTGATGAGCATTTGCCGCCAGTGGTCCTATTTAAAAACGCTGATTCAGACGCCA > minE/2394508‑2394644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |