Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	705,870	0	A	G	38.5%	5.7 / 10.3	13	intergenic (‑367/+128)	ymcC/serX	predicted outer membrane lipoprotein/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (6/2);  new base G (2/3);  total (8/5)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.21e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCC  >  minE/705850‑705938                     |                                                                     gcCCTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTTTCCAACTGGCTGTCGCCAGTTGTCGAACCCCg                      <  1:159000/69‑1 (MQ=255) gcCCTGCGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCg                      <  1:1771687/69‑1 (MQ=25) gcCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGt                    >  1:5511/1‑71 (MQ=34)  cccTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTc                   >  1:523113/1‑71 (MQ=34)       cGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGc              >  1:2127449/1‑71 (MQ=21)       cGGGCAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGc              <  1:707170/71‑1 (MQ=21)       cGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCggg                <  1:2054050/69‑1 (MQ=35)       cGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCggg                <  1:303988/69‑1 (MQ=35)           cAGCCCGCTCGCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTtc           >  1:2739004/1‑70 (MQ=25)                cGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATcc     >  1:2958431/1‑71 (MQ=34)                 gCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCtt            >  1:2746742/1‑63 (MQ=35)                 gCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATccc    >  1:67708/1‑71 (MQ=34)                  cTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATcccc   >  1:607025/1‑71 (MQ=34)                     aCTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATccccc  <  1:1559152/69‑1 (MQ=35)                     |                                                                     GCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCC  >  minE/705850‑705938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑