Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,000,186	G→A	G179D (GGT→GAT)	ydgD →	predicted peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,000,186	0	G	A	100.0%	155.8 / NA	53	G179D (GGT→GAT)	ydgD	predicted peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/30);  total (23/30)

CATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTG  >  minE/1000121‑1000252                                                                  |                                                                   cATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGt                                                                >  1:929518/1‑70 (MQ=255) cATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTa                                                               <  1:2295206/71‑1 (MQ=255) cATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTa                                                               <  1:2291653/71‑1 (MQ=255)   ttACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTaaa                                                             <  1:987012/71‑1 (MQ=255)     aCGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAg                                                            >  1:2556745/1‑70 (MQ=255)         cgttgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACt                                                       <  1:661553/71‑1 (MQ=255)           ttgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTa                                                               <  1:834231/61‑1 (MQ=255)           ttgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACt                                                       >  1:1678831/1‑69 (MQ=255)            tgccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTcag                                                    >  1:1088118/1‑71 (MQ=255)              ccgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTcaggc                                                  <  1:737512/71‑1 (MQ=255)               cgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTcaggca                                                 <  1:410305/71‑1 (MQ=255)               cgttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTcaggca                                                 <  1:1734593/71‑1 (MQ=255)                 ttATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTaaa                                                             >  1:2916831/1‑57 (MQ=255)                   aTTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCaggcaggc                                              >  1:1038845/1‑70 (MQ=255)                   aTTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCaggcaggc                                              <  1:1394531/70‑1 (MQ=255)                    tttGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTa                                            <  1:2120594/71‑1 (MQ=255)                         gggAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTg                                       <  1:2773570/71‑1 (MQ=255)                            agaTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTca                                                     <  1:2641702/54‑1 (MQ=255)                            agaTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTca                                                     <  1:855649/54‑1 (MQ=255)                              aTAAAGCCGCGCTTACCGCCTCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTg                                       >  1:886121/1‑66 (MQ=255)                              aTAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTg                                       >  1:2797670/1‑66 (MQ=255)                                  aGCCGCGCTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGatcatc                              <  1:159264/71‑1 (MQ=255)                                        gcTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATa                        >  1:2353750/1‑71 (MQ=255)                                        gcTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATa                        >  1:1231365/1‑71 (MQ=255)                                         cTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAg                                    <  1:2281060/58‑1 (MQ=255)                                         cTTACCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATAc                       >  1:1446157/1‑71 (MQ=255)                                         cTTACCGCCGCATTAAAAGCGGAAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAg                                    <  1:2178122/58‑1 (MQ=255)                                            aCCGCCGCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATa                        >  1:142093/1‑67 (MQ=255)                                             ccgccgCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGaa                                     >  1:1425197/1‑53 (MQ=255)                                             ccgccgCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGtt                    <  1:1442586/70‑1 (MQ=255)                                             ccgccgCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGtt                    <  1:1056294/70‑1 (MQ=255)                                             ccgccgCATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTg                   <  1:967796/71‑1 (MQ=255)                                              cgccgcATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTg                   >  1:92718/1‑70 (MQ=255)                                                ccgcATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTa                 >  1:2338753/1‑70 (MQ=255)                                                ccgcATTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTAc                <  1:1088861/71‑1 (MQ=255)                                                    aTTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGAt                         >  1:2960120/1‑58 (MQ=255)                                                    aTTAAAAGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGt             >  1:2625384/1‑70 (MQ=255)                                                       aaaaGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGatcat                               >  1:2378516/1‑49 (MQ=255)                                                       aaaaGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGtcat          >  1:1895677/1‑70 (MQ=255)                                                        aaaGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTGGATACGTTg                   <  1:2083442/60‑1 (MQ=255)                                                        aaaGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGAt                         <  1:2502114/54‑1 (MQ=255)                                                        aaaGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGtcatc         <  1:59619/70‑1 (MQ=255)                                                         aaGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCaa       <  1:1362202/71‑1 (MQ=255)                                                          aGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGt                     >  1:2750045/1‑56 (MQ=255)                                                          aGCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCaaa      <  1:2939294/71‑1 (MQ=255)                                                           gCGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCaaaa     >  1:1078946/1‑71 (MQ=255)                                                            cGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGatcatat                             <  1:2088803/46‑3 (MQ=255)                                                            cGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCAtct                             <  1:507079/46‑1 (MQ=255)                                                            cGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCAtct                             <  1:2970965/46‑1 (MQ=255)                                                            cGGCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCg                           <  1:1799653/48‑1 (MQ=255)                                                              gCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCAtctc                            <  1:1136121/45‑1 (MQ=255)                                                              gCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACtg  >  1:1732842/1‑71 (MQ=255)                                                              gCAGATCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACtg  >  1:1736466/1‑71 (MQ=255)                                                                  |                                                                   CATTACGCCGTTGCCGTTATTTGAGGGAGATAAAGCCGCGCTTACCGCCGCATTAAAAGCGGCAGGTCGTAAAGTGACTCAGGCAGGCTACCCTGAAGATCATCTCGATACGTTGTACAGTCATCAAAACTG  >  minE/1000121‑1000252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑