Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,013,547	G→A	R245C (CGC→TGC)	fumC ←	fumarate hydratase (fumarase C), aerobic Class II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,013,547	0	G	A	100.0%	155.8 / NA	52	R245C (CGC→TGC)	fumC	fumarate hydratase (fumarase C), aerobic Class II
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/31);  total (21/31)

CGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGC  >  minE/1013479‑1013613                                                                     |                                                                   cgcTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcaac                                                                  >  1:3025919/1‑71 (MQ=255)  gcTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcaacg                                                                 <  1:2553492/71‑1 (MQ=255)      cAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCa                                                             >  1:1322285/1‑71 (MQ=255)       aaaTTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCa                                                             >  1:1951836/1‑70 (MQ=255)       aaaTTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCa                                                             <  1:618728/70‑1 (MQ=255)       aaaTTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCa                                                             >  1:356045/1‑70 (MQ=255)         aTTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATAc                                                          >  1:16689/1‑71 (MQ=255)            tGTTCGGCGCGGTAACAAACAGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcaac                                                                  >  1:2438075/1‑60 (MQ=255)             gTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCg                                                      >  1:1588092/1‑71 (MQ=255)              ttCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCgg                                                     <  1:2132342/71‑1 (MQ=255)                 ggCGCGGTATCAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGAt                                                   <  1:2279520/70‑1 (MQ=255)                 ggCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGAt                                                   <  1:1099967/70‑1 (MQ=255)                 ggCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGAt                                                   <  1:2363764/70‑1 (MQ=255)                 ggCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGAt                                                   <  1:2520364/70‑1 (MQ=255)                  gcgcGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATgg                                                 >  1:128949/1‑71 (MQ=255)                       gTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGAt                                                   <  1:2929969/64‑1 (MQ=255)                       gTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTAtt                                            <  1:528083/71‑1 (MQ=255)                       gTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATAATCCGGATGGGTAtt                                            <  1:2361639/71‑1 (MQ=255)                        tAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTAtt                                            >  1:251127/1‑70 (MQ=255)                        tAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTAtt                                            >  1:2059628/1‑70 (MQ=255)                          aCAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAg                                         <  1:1155707/71‑1 (MQ=255)                           cAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGt                                        >  1:1868614/1‑71 (MQ=255)                            aaaCGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTc                                       <  1:983848/71‑1 (MQ=255)                            aaaCGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTc                                       <  1:998198/71‑1 (MQ=255)                            aaaCGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTc                                       <  1:1180832/71‑1 (MQ=255)                             aaCGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTcc                                      <  1:1682595/71‑1 (MQ=255)                              aCGGTGCACAGGTAATGACTGCCAGTTCATCTGCTacgcaacgag                                                               >  1:580358/1‑43 (MQ=255)                              aCGGTGCACAGGTAATGACTGACAGTTCATCTTCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCa                                     <  1:191290/71‑1 (MQ=255)                               cGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCa                                     <  1:411134/70‑1 (MQ=255)                                 gTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTa                                  <  1:765725/71‑1 (MQ=255)                                  tGCACAGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTcc                                      <  1:1719464/66‑1 (MQ=255)                                    cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCTGATGGGTATTTAGTCCAGTAccc                               <  1:986004/71‑1 (MQ=255)                                    cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc                               <  1:1722157/71‑1 (MQ=255)                                    cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc                               <  1:761504/71‑1 (MQ=255)                                    cacaGGTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTAccc                               <  1:2191138/71‑1 (MQ=255)                                        ggTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCg                           <  1:791533/71‑1 (MQ=255)                                        ggTAATGACTGCCAGTTCATCTGCTACGCAAAGCGCATACTCCGGa                                                    >  1:852225/1‑46 (MQ=255)                                         gTAATGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGc                          >  1:1156337/1‑71 (MQ=255)                                           aaTGACTGCCAGTTAATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTg                        >  1:2226118/1‑71 (MQ=255)                                             tGACTGCCAGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTAtt                                            <  1:2221543/49‑1 (MQ=255)                                                     aGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTa                                  >  1:2548899/1‑51 (MQ=255)                                                     aGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGcc                 >  1:926558/1‑68 (MQ=255)                                                     aGTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGcc                 >  1:456182/1‑68 (MQ=255)                                                      gTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTAtt                                            <  1:2952482/40‑1 (MQ=255)                                                      gTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTcc                                      >  1:1678669/1‑46 (MQ=255)                                                      gTTCATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCaa               >  1:2280514/1‑69 (MQ=255)                                                         cATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGc           <  1:192715/70‑1 (MQ=255)                                                         cATCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGc           >  1:1949475/1‑70 (MQ=255)                                                          aTCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGcc          <  1:1108921/70‑1 (MQ=255)                                                          aTCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGcc          <  1:285884/70‑1 (MQ=255)                                                          aTCTGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGcc          <  1:2798708/70‑1 (MQ=255)                                                             tGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGtt      >  1:1710135/1‑71 (MQ=255)                                                             tGCTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGtt      >  1:1951200/1‑71 (MQ=255)                                                               cTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCTCCAAGAGCCAg        <  1:2499925/67‑1 (MQ=255)                                                               cTACGCAACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTcc    <  1:2352807/71‑1 (MQ=255)                                                                tACGCAACGCACATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCg   >  1:2989911/1‑71 (MQ=255)                                                                  cgcaacgcgCATACTCCGGATGGGTATTTAGTCCAGTa                                  <  1:2176853/38‑1 (MQ=255)                                                                  cgcaacgcgCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCg   <  1:319568/69‑1 (MQ=255)                                                                  cgcaacgcgCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGc  >  1:664349/1‑70 (MQ=255)                                                                     |                                                                   CGCTTCAAATTTGTTCGGCGCGGTAACAAACGGTGCACAGGTAATGACTGCCAGTTCATCTGCTACGCGACGCGCATACTCCGGATGGGTATTTAGTCCAGTACCCACCGCTGTACCGCCAAGAGCCAGTTCCGC  >  minE/1013479‑1013613

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑