Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,081,858	T→C	N104S (AAC→AGC)	ydhY ←	predicted 4Fe‑4S ferridoxin‑type protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,081,858	0	T	C	100.0%	140.5 / NA	40	N104S (AAC→AGC)	ydhY	predicted 4Fe‑4S ferridoxin‑type protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/17);  total (23/17)

GCATTGACGGCAGGTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTACTGAGCCATCGT  >  minE/1081791‑1081924                                                                    |                                                                   gCATTGACGGCAGGTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTg                                                                  <  1:851358/70‑1 (MQ=255)       aCGGCAGGTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCa                                                            >  1:2569337/1‑70 (MQ=255)        cGGCAGGTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCa                                                            >  1:1366074/1‑69 (MQ=255)                 tCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                                 <  1:220947/71‑1 (MQ=255)                  ccGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGa                                                        <  1:3031051/63‑1 (MQ=255)                     cgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGcc                                                             <  1:2652834/55‑1 (MQ=255)                     cgGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGa                                             <  1:2383919/71‑1 (MQ=255)                         ataGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaat                                                     >  1:2403954/1‑59 (MQ=255)                         ataGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaat                                                     >  1:543125/1‑59 (MQ=255)                          taGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataa                                                   >  1:461372/1‑60 (MQ=255)                          taGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGAt                                        >  1:2427881/1‑71 (MQ=255)                            gTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGa                                         <  1:2914329/68‑1 (MQ=255)                            gTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGAttt                                      >  1:220294/1‑71 (MQ=255)                             ttGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGAtt                                       <  1:1972120/69‑1 (MQ=255)                              tGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaat                                                     <  1:2392164/54‑1 (MQ=255)                               gagaTCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGa                                   >  1:900849/1‑71 (MQ=255)                                   tCGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACg                               <  1:794029/71‑1 (MQ=255)                                    cGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAAtt                                                 >  1:2227030/1‑52 (MQ=255)                                    cGCCATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACgg                              >  1:2836534/1‑71 (MQ=255)                                    cGCCATACAGGCCGCCGCCAGAGCCAACACCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACgg                              >  1:1510196/1‑71 (MQ=255)                                      ccATACAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACgg                              >  1:1787967/1‑69 (MQ=255)                                           cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAaataa                                                   >  1:689874/1‑43 (MQ=255)                                           cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaaga                       <  1:1051884/71‑1 (MQ=255)                                           cAGGCCGCCGCCAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag                        >  1:2350988/1‑70 (MQ=255)                                                cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc                  >  1:1962613/1‑71 (MQ=255)                                                cgccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTc                  <  1:1118197/71‑1 (MQ=255)                                                  ccgccAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTccc                >  1:1084633/1‑71 (MQ=255)                                                      cAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGTGAGAAGAATGTCCCTACt            <  1:278968/71‑1 (MQ=255)                                                      cAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGagaag                        <  1:2194644/59‑1 (MQ=255)                                                      cAGAGCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCTTACt            >  1:1528286/1‑71 (MQ=255)                                                       agagCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTACt            >  1:2279985/1‑70 (MQ=255)                                                        gagCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCATACt            >  1:1052412/1‑69 (MQ=255)                                                          gCCAACCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTACTGAGc        <  1:1440976/71‑1 (MQ=255)                                                             aaCCCCGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTACTGAGCCAt     >  1:1512162/1‑71 (MQ=255)                                                               ccccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACgg                              <  1:1012846/44‑1 (MQ=255)                                                               ccccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACgg                              <  1:1016603/44‑1 (MQ=255)                                                               ccccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTACTGATCCATCg   >  1:1782735/1‑71 (MQ=255)                                                               ccccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTACTGAGCCATCg   >  1:2323402/1‑71 (MQ=255)                                                                cccGCTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTACTGAGCCATCGt  >  1:852244/1‑71 (MQ=255)                                                                cccGCTGTCGCCAAAGAAATAATTGAGATGGATTTTGATACGGGAGAAGAATGTCCCTACTGAGCCATCGt  <  1:171860/71‑1 (MQ=255)                                                                    |                                                                   GCATTGACGGCAGGTGTCCGCGGTATAGTTGAGATCGCCATACAGGCCGCCGCCAGAGCCAACCCCGTTGTCGCCAAAGAAATAATTGCGATGGATTTTGATACGGGAGAAGAATGTCCCTACTGAGCCATCGT  >  minE/1081791‑1081924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑