Predicted mutation
evidence seq id position mutation annotation gene description
RA minE 1,097,909 T→C intergenic (+32/‑397) ydiK → / → ydiL predicted inner membrane protein/conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,097,9090TC100.0% 94.8 / NA 28intergenic (+32/‑397)ydiK/ydiLpredicted inner membrane protein/conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/12);  total (16/12)

GGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGG  >  minE/1097851‑1097975
                                                          |                                                                  
ggCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGCt                                                         <  1:1710447/70‑1 (MQ=255)
     aaTCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTa                                                        >  1:2125572/1‑66 (MQ=255)
      aTCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGa                                                   >  1:1181350/1‑70 (MQ=255)
           gaAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATttg                                             >  1:1756079/1‑71 (MQ=255)
                 gAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATttgcttgc                                        >  1:1646449/1‑70 (MQ=255)
                   aTAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGctct                                     >  1:1732254/1‑71 (MQ=255)
                       gTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTaa                                                                 <  1:2283590/39‑1 (MQ=255)
                        tAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGctct                                     <  1:285064/66‑1 (MQ=255)
                               tcatcaGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGctct                                     <  1:3035700/59‑1 (MQ=255)
                                 atcaGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTcc                       >  1:1524076/1‑71 (MQ=255)
                                    aGGCGGCTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTc                        <  1:1414068/67‑1 (MQ=255)
                                        ggcTCTGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTAc                >  1:1957823/1‑71 (MQ=255)
                                          ctctGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTccc                                 <  1:2872788/52‑1 (MQ=255)
                                           tctGCCGCCTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTAc                >  1:2917813/1‑68 (MQ=255)
                                               ccgccTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCAtt         >  1:2576015/1‑71 (MQ=255)
                                               ccgccTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCAt          >  1:2058740/1‑70 (MQ=255)
                                                cgccTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCatat                            <  1:975090/51‑1 (MQ=255)
                                                cgccTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCAtt         >  1:1854341/1‑70 (MQ=255)
                                                  ccTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATAttt                          >  1:2512181/1‑51 (MQ=255)
                                                  ccTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAg      <  1:1302568/71‑1 (MQ=255)
                                                   cTGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAg      >  1:747574/1‑70 (MQ=255)
                                                    tGATTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCt                   >  1:550927/1‑56 (MQ=255)
                                                      aTTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCatat                            <  1:2367041/45‑1 (MQ=255)
                                                      aTTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCatat                            <  1:1807856/45‑1 (MQ=255)
                                                      aTTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCatat                            <  1:1233391/45‑1 (MQ=255)
                                                      aTTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATAttt                          >  1:1274532/1‑47 (MQ=255)
                                                      aTTGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTgg  >  1:116086/1‑71 (MQ=255)
                                                       ttGCTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTgg  <  1:712062/70‑1 (MQ=255)
                                                          |                                                                  
GGCGAAATCGAGAAACCGAATAAGTAATTTCTCATCAGGCGGCTCTGCCGCCTGATTGTTAACCACCGCTAATTGATATTTGCTTGCTCTTCCCCATATTTTCCTGCTTACCGCCATTCAGCTGG  >  minE/1097851‑1097975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: