| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,099,149 | A→G | T130A (ACA→GCA) | ydiM → | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,099,149 | 0 | A | G | 96.4% | 90.0 / ‑3.0 | 28 | coding (388/1215 nt) | ydiM | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (16/11); minor base . (1/0); total (17/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
GCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATCATT > minE/1099083‑1099211 | gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTGACCTGGGGCAGc > 1:1413847/1‑71 (MQ=255)gCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGc > 1:158715/1‑71 (MQ=255) cAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGcc < 1:1864218/71‑1 (MQ=255) gTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAatat < 1:2200469/70‑1 (MQ=255) ttCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCa > 1:786987/1‑59 (MQ=255) ttCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATAtttt > 1:1776910/1‑70 (MQ=255) ttCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGG‑CAGCCAATATTttaa > 1:2017238/1‑71 (MQ=255) tcGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAatat < 1:63053/64‑1 (MQ=255) tcGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAatat < 1:2580629/64‑1 (MQ=255) cTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGtt > 1:2740968/1‑71 (MQ=255) tATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCa > 1:1328918/1‑42 (MQ=255) aTCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTttaat > 1:1827366/1‑55 (MQ=255) tCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCa < 1:2089552/71‑1 (MQ=255) ccAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGc > 1:2233324/1‑71 (MQ=255) cAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAg > 1:303466/1‑69 (MQ=255) gTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCAtt < 1:1213765/58‑1 (MQ=255) gTTTGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGa > 1:137580/1‑71 (MQ=255) tGATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGttt > 1:1470736/1‑60 (MQ=255) gATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACaa < 1:412110/70‑1 (MQ=255) gATGGAAGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACaa < 1:1191882/70‑1 (MQ=255) aaGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTtta > 1:1755902/1‑71 (MQ=255) aGCTTTTCCACGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTttat > 1:3020983/1‑71 (MQ=255) aCGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAttt > 1:2231814/1‑58 (MQ=255) aCGCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtt > 1:355498/1‑57 (MQ=255) gCTCACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATc < 1:91988/71‑1 (MQ=255) cACCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAAtttt > 1:2811815/1‑54 (MQ=255) aCCTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATCAtt < 1:1277922/70‑1 (MQ=255) ccTGGGGCAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATCAt < 1:1450711/68‑1 (MQ=255) | GCAAACAGTTTTCTCGATGCAGGCACTTATCCCAGTTTGATGGAAGCTTTTCCACGCTCACCTGGGACAGCCAATATTTTAATTAAAGCATTTGTTTCCAGCGGACAATTTTTATTACCGCTAATCATT > minE/1099083‑1099211 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |