Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,151,211	A→G	V2V (GTA→GTG)	ydjQ →	endonuclease of nucleotide excision repair

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,151,211	0	A	G	100.0%	111.3 / NA	32	V2V (GTA→GTG)	ydjQ	endonuclease of nucleotide excision repair
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/17);  total (15/17)

GCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCGAACATTT  >  minE/1151143‑1151276                                                                     |                                                                  gCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCg                                                                 <  1:2787109/71‑1 (MQ=255)   ctctTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGc                                                               >  1:2640552/1‑71 (MQ=255)    tctTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTgcggcg                                                              >  1:2427823/1‑71 (MQ=255)      ttttttAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGtt                                                            >  1:1301406/1‑71 (MQ=255)      ttttttAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGtt                                                            >  1:1885116/1‑71 (MQ=255)        ttttAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGt                                                             <  1:1748680/68‑1 (MQ=255)         tttAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGtt                                                            <  1:2685479/68‑1 (MQ=255)         tttAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTa                                                          <  1:1009348/70‑1 (MQ=255)         tttAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTa                                                          <  1:471471/70‑1 (MQ=255)             aTCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGTTGCGGCGTTTAACtt                                                      <  1:2049595/70‑1 (MQ=255)             aTCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTtc                                                     <  1:1232601/71‑1 (MQ=255)             aTCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTtc                                                     >  1:509434/1‑71 (MQ=255)                gCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTgcggcg                                                              <  1:769890/59‑1 (MQ=255)                gCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTcc                                                  >  1:2573779/1‑71 (MQ=255)                  ttCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGtt                                                            >  1:2794031/1‑59 (MQ=255)                  ttCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCcgc                                                >  1:843312/1‑71 (MQ=255)                     ccGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTa                                                          <  1:186094/58‑1 (MQ=255)                     ccGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCgg                                              <  1:1792220/70‑1 (MQ=255)                          tGTTACACTGGATAGATAACCAGCATTCGGAGTCAAGAGTGGTGCGGCGttt                                                           >  1:303416/1‑52 (MQ=255)                               cacTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTg                                    <  1:2775777/70‑1 (MQ=255)                                    gatagataACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTg                              <  1:1660993/71‑1 (MQ=255)                                    gatagataACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTg                              >  1:2374303/1‑71 (MQ=255)                                      tagataACCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCg                            <  1:1112581/71‑1 (MQ=255)                                           aaCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                        >  1:2416614/1‑38 (MQ=255)                                           aaCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                        >  1:2079801/1‑38 (MQ=255)                                           aaCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                        >  1:297140/1‑38 (MQ=255)                                           aaCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAAc                                                        >  1:1420838/1‑38 (MQ=255)                                            aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAtt                      <  1:1762793/71‑1 (MQ=255)                                            aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAtt                      <  1:1759127/71‑1 (MQ=255)                                            aCCAGCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAAtt                      <  1:1287296/71‑1 (MQ=255)                                                gCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATg                  <  1:534897/71‑1 (MQ=255)                                                gCATTCGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATg                  <  1:2042058/71‑1 (MQ=255)                                                     cGGAGTCAACAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCg                            >  1:1144204/1‑56 (MQ=255)                                                              cAGTGGTGCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCGAACAt    >  1:1583626/1‑71 (MQ=255)                                                                gtggtgCGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCGAACAttt  <  1:1910308/71‑1 (MQ=255)                                                                     |                                                                  GCCTCTTTTTTAATCGCTTCCCGCCTGTTACACTGGATAGATAACCAGCATTCGGAGTCAACAGTGGTACGGCGTTTAACTTCTCCGCGGCTCGAATTTGAAGCTGCGGCAATTTATGAATATCCCGAACATTT  >  minE/1151143‑1151276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑