Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	91,462	T→C	S56P (TCT→CCT)	lpxC →	UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	91,462	0	T	C	100.0%	209.8 / NA	58	S56P (TCT→CCT)	lpxC	UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/21);  total (37/21)

ACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAG  >  minE/91397‑91528                                                                  |                                                                   acacCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCtgtg                                                               >  1:1631487/1‑71 (MQ=255)   acCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCg                                                             >  1:1780043/1‑71 (MQ=255)    ccGGTGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGt                                                            <  1:358161/71‑1 (MQ=255)     cGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGt                                                            <  1:755567/70‑1 (MQ=255)        ggTCATCTATCGTCGCACCGACTTGAATCCACCGGGAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATa                                                        >  1:1747357/1‑71 (MQ=255)            aTCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCAt                                                    >  1:1267482/1‑71 (MQ=255)                 tcgtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATtctc                                                <  1:2945983/70‑1 (MQ=255)                  cgtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGt                                                            >  1:1468420/1‑57 (MQ=255)                   gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                              <  1:2411522/70‑1 (MQ=255)                   gtcgCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCAAAACCTGTGCGTGATAACATGCTCTg                                              >  1:2278716/1‑70 (MQ=255)                      gCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTAc                                           <  1:2224686/70‑1 (MQ=255)                      gCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTAc                                           <  1:2520790/70‑1 (MQ=255)                         ccGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtgt                                       >  1:1460820/1‑71 (MQ=255)                         ccGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACAATGCTCTGTACgtgt                                       >  1:2513700/1‑71 (MQ=255)                          cGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATg                                                   >  1:1001050/1‑58 (MQ=255)                          cGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTc                                      <  1:437177/71‑1 (MQ=255)                              ttGAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATAcc                                                      <  1:2233433/51‑1 (MQ=255)                                gAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGa                                                          >  1:682771/1‑45 (MQ=255)                                gAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGa                                                          >  1:1127465/1‑45 (MQ=255)                                gAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACTTGTCTGGTc                                 >  1:2813097/1‑70 (MQ=255)                                gAATCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTc                                 >  1:576551/1‑70 (MQ=255)                                 aaTCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGa                                                          >  1:48709/1‑44 (MQ=255)                                 aaTCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGa                                                          >  1:2147302/1‑44 (MQ=255)                                 aaTCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgtgt                                       <  1:566223/63‑1 (MQ=255)                                 aaTCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCaa                               <  1:2212484/71‑1 (MQ=255)                                 aaTCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCa                                <  1:2469324/70‑1 (MQ=255)                                  aTCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAAc                              >  1:2805225/1‑71 (MQ=255)                                  aTCCACCGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACATGTCTGGTCAAc                              >  1:1600025/1‑71 (MQ=255)                                       ccGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCaa                               >  1:1930562/1‑65 (MQ=255)                                        cGGTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGt                                  >  1:1015339/1‑61 (MQ=255)                                         ggTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTg                                              <  1:81340/48‑1 (MQ=255)                                         ggTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatg                       <  1:2473613/71‑1 (MQ=255)                                          gTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatga                      <  1:987887/71‑1 (MQ=255)                                          gTAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatga                      >  1:1725524/1‑71 (MQ=255)                                           tAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACgt                                         <  1:2773807/51‑1 (MQ=255)                                           tAGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatgat                     >  1:2999218/1‑71 (MQ=255)                                            aGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTa                                            >  1:1942676/1‑47 (MQ=255)                                            aGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatgatg                    >  1:2201741/1‑71 (MQ=255)                                            aGATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatgatg                    >  1:2899454/1‑71 (MQ=255)                                             gATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatgatg                    <  1:1948175/70‑1 (MQ=255)                                             gATTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatgatg                    <  1:587515/70‑1 (MQ=255)                                              aTTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTa                  >  1:2725110/1‑71 (MQ=255)                                              aTTTCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTa                  >  1:1126525/1‑71 (MQ=255)                                               tttCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCa                                <  1:1402262/56‑1 (MQ=255)                                                ttCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACg                >  1:420609/1‑71 (MQ=255)                                                 tCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCatgat                     >  1:1586585/1‑65 (MQ=255)                                                 tCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACg                >  1:1870116/1‑70 (MQ=255)                                                 tCCCGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACg                >  1:1739097/1‑70 (MQ=255)                                                   ccGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGAt             <  1:1625720/71‑1 (MQ=255)                                                    cGGCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACg                                          >  1:1321926/1‑41 (MQ=255)                                                      gCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTa                  >  1:755089/1‑63 (MQ=255)                                                      gCCGATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGAttt           >  1:2324089/1‑70 (MQ=255)                                                         gATGCCAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACgg               <  1:1803644/63‑1 (MQ=255)                                                              cAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCa                         >  1:552094/1‑48 (MQ=255)                                                              cAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCg     >  1:1623930/1‑68 (MQ=255)                                                              cAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCg     >  1:948117/1‑68 (MQ=255)                                                              cAAACCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTag  <  1:798860/71‑1 (MQ=255)                                                               aaaCCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAAc                              >  1:824793/1‑42 (MQ=255)                                                                  |                                                                   ACACCGGGGTCATCTATCGTCGCACCGACTTGAATCCACCGGTAGATTTCCCGGCCGATGCCAAATCTGTGCGTGATACCATGCTCTGTACGTGTCTGGTCAACGAGCATGATGTACGGATTTCAACCGTAG  >  minE/91397‑91528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑