Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,253,716	C→T	A241A (GCG→GCA)	aspS ←	aspartyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,253,716	0	C	T	100.0%	201.9 / NA	67	A241A (GCG→GCA)	aspS	aspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/36);  total (31/36)

AGATCCACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTC  >  minE/1253648‑1253781                                                                     |                                                                  aGATCCACACCCTTCACTTCCAGCCACAGATGACGCACCGGCGCTTCCAACACTTCACGCACTTGCGgtgc                                                                 >  1:1731622/1‑71 (MQ=255) aGATCCACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGgtgc                                                                 >  1:1804426/1‑71 (MQ=255) aGATCCACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGgtgc                                                                 >  1:1562876/1‑71 (MQ=255)  gATCCACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGtgcg                                                                >  1:720661/1‑71 (MQ=255)  gATCCACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGtgcg                                                                >  1:1301015/1‑71 (MQ=255)  gATCCACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGtgcg                                                                <  1:1303805/71‑1 (MQ=255)     ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTgcggtgcggt                                                              >  1:2799644/1‑70 (MQ=255)     ccACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTgcggtgcggt                                                              >  1:1942545/1‑70 (MQ=255)       acacCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCAt                                                           <  1:1706760/71‑1 (MQ=255)          cccTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGaa                                                        <  1:1445581/71‑1 (MQ=255)           ccTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGaa                                                        <  1:2728486/70‑1 (MQ=255)            cTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAaag                                                      <  1:365737/71‑1 (MQ=255)            cTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAaag                                                      <  1:2592531/71‑1 (MQ=255)               cACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAaagaag                                                   >  1:418832/1‑71 (MQ=255)                aCTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGt                                                  >  1:352467/1‑71 (MQ=255)                aCTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGt                                                  >  1:807475/1‑71 (MQ=255)                    ccAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTc                                               >  1:2875039/1‑70 (MQ=255)                    ccAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTc                                               <  1:2571074/70‑1 (MQ=255)                    ccAGACACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTc                                               >  1:1807970/1‑70 (MQ=255)                    acAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGttt                                                >  1:2279823/2‑69 (MQ=255)                        ccACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCAt                                                           >  1:2093468/1‑54 (MQ=255)                         cacaGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCcac                                            <  1:1342006/68‑1 (MQ=255)                         cacaGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATc                                         >  1:1287611/1‑71 (MQ=255)                          acaGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATg                                                          >  1:2836809/1‑53 (MQ=255)                          acaGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATg                                                          >  1:2178203/1‑53 (MQ=255)                            aGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGAt                                      <  1:125159/71‑1 (MQ=255)                            aGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGAt                                      <  1:870510/71‑1 (MQ=255)                            aGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGAt                                      <  1:770790/71‑1 (MQ=255)                             gATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATc                                     >  1:1433706/1‑71 (MQ=255)                             gATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATc                                     >  1:949945/1‑71 (MQ=255)                             gATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATc                                     <  1:1195909/71‑1 (MQ=255)                                   gCACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGt                                >  1:2058832/1‑70 (MQ=255)                                    cACCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTaa                              <  1:1822276/71‑1 (MQ=255)                                     tccAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTc                                               <  1:1649538/52‑1 (MQ=255)                                     aCCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTaaa                             >  1:1903456/1‑71 (MQ=255)                                     aCCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTaaa                             <  1:2554548/71‑1 (MQ=255)                                     aCCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTaaa                             >  1:33509/1‑71 (MQ=255)                                     aCCAGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTaa                              <  1:3030746/70‑1 (MQ=255)                                        aGCGCTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTc                          <  1:1176658/71‑1 (MQ=255)                                         gcgcTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAaag                                                      <  1:134556/42‑1 (MQ=255)                                         gcgcTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCa                         <  1:1914051/71‑1 (MQ=255)                                         gcgcTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCa                         <  1:2969177/71‑1 (MQ=255)                                          cgcTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAg                        >  1:1525672/1‑71 (MQ=255)                                            cTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTc                          <  1:484182/67‑1 (MQ=255)                                            cTTCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTc                          <  1:1995857/67‑1 (MQ=255)                                             ttCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTaaa                             <  1:842144/63‑1 (MQ=255)                                             ttCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCt                     >  1:1028097/1‑71 (MQ=255)                                              tCCATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTg                                   <  1:2980369/56‑1 (MQ=255)                                               ccATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAAtt                           <  1:1032898/63‑1 (MQ=255)                                               ccATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGa                   >  1:513998/1‑71 (MQ=255)                                               ccATCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGa                   >  1:890781/1‑71 (MQ=255)                                                 aTCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCg                                        <  1:81009/48‑1 (MQ=255)                                                  tCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGAt                                      <  1:2556945/49‑1 (MQ=255)                                                  tCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGAt                                      <  1:2480134/49‑1 (MQ=255)                                                  tCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAAtt                           <  1:2341147/60‑1 (MQ=255)                                                  tCACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGa                   >  1:2465160/1‑68 (MQ=255)                                                   cACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGt               >  1:662552/1‑71 (MQ=255)                                                   cACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGt               >  1:715190/1‑71 (MQ=255)                                                   cACTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGt               >  1:1762162/1‑71 (MQ=255)                                                    aCTTCACGCACTTGCGGTGCTGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGt               <  1:2612071/70‑1 (MQ=255)                                                    aCTTCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGt               >  1:649558/1‑70 (MQ=255)                                                       tCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCt                     <  1:2177496/61‑1 (MQ=255)                                                       tCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg            >  1:1332686/1‑70 (MQ=255)                                                       tCACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg            >  1:1990872/1‑70 (MQ=255)                                                        cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCt                     <  1:1171670/60‑1 (MQ=255)                                                        cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTg                    >  1:2914406/1‑61 (MQ=255)                                                        cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTg                    >  1:199554/1‑61 (MQ=255)                                                        cACGCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAg            <  1:2664085/69‑1 (MQ=255)                                                           gCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGc       <  1:1009183/71‑1 (MQ=255)                                                           gCACTTGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGc       <  1:98427/71‑1 (MQ=255)                                                               ttGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGa                                  >  1:900590/1‑40 (MQ=255)                                                               ttGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGa                                  >  1:107112/1‑40 (MQ=255)                                                               ttGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACg                 <  1:959338/57‑1 (MQ=255)                                                               ttGCGGTGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGt   >  1:26540/1‑71 (MQ=255)                                                                tgcggtgcggTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTc  >  1:1206859/1‑71 (MQ=255)                                                                tGCGGTGCTGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACg                 <  1:1929360/56‑1 (MQ=255)                                                                     |                                                                  AGATCCACACCCTTCACTTCCAGCCACAGATGACGCACCAGCGCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTC  >  minE/1253648‑1253781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑