Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,302,228	C→T	V285M (GTG→ATG)	dacD ←	D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,302,228	0	C	T	100.0%	159.5 / NA	53	V285M (GTG→ATG)	dacD	D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/21);  total (32/21)

GATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTG  >  minE/1302160‑1302290                                                                     |                                                               gATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCg                                                              <  1:2583125/71‑1 (MQ=255)  atatTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCg                                                              >  1:2998405/1‑70 (MQ=255)     ttttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCgtag                                                           <  1:1560916/70‑1 (MQ=255)     ttttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCgtag                                                           >  1:1195130/1‑70 (MQ=255)     ttttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCgtag                                                           >  1:582555/1‑70 (MQ=255)       ttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCgtagta                                                         >  1:1685196/1‑70 (MQ=255)       ttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCgtagta                                                         <  1:1370530/70‑1 (MQ=255)       ttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCgta                                                            >  1:1020082/1‑67 (MQ=255)       ttCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCgta                                                            >  1:542020/1‑67 (MQ=255)         cTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCgtagta                                                         >  1:1191471/1‑68 (MQ=255)            tATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAg                                                      <  1:2142111/68‑1 (MQ=255)             aTCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCg                                                              <  1:582194/59‑1 (MQ=255)              tCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAg                                                      >  1:2891415/1‑66 (MQ=255)              tCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGtttt                                                  <  1:2603931/70‑1 (MQ=255)                gCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTttgt                                                <  1:2268129/70‑1 (MQ=255)                          aTGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGtttt                                                  <  1:1630407/58‑1 (MQ=255)                          aTGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCaa                                       <  1:976865/69‑1 (MQ=255)                            gCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGccc                                           >  1:2424276/1‑63 (MQ=255)                              gttccgttccGACCTTTTTCCCACGGTGCAAAATTTGCATCg                                                              <  1:42578/42‑1 (MQ=255)                                 ccgttccgACCTTTTTCCCACGGTGCAAAATTTGCATCgta                                                            >  1:2729451/1‑41 (MQ=255)                                 ccgttccgACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTttgt                                                >  1:837054/1‑53 (MQ=255)                                 ccgttccgACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTaa                               >  1:73959/1‑70 (MQ=255)                                  cgttccgACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAAtt                             <  1:2303877/71‑1 (MQ=255)                                   gttccgACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACg                                     <  1:2577734/62‑1 (MQ=255)                                      ccgACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTttg                                                 >  1:1472464/1‑47 (MQ=255)                                      ccgACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTttg                                                 >  1:166218/1‑47 (MQ=255)                                      ccgACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCt                         >  1:25178/1‑71 (MQ=255)                                      ccgACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCt                         >  1:1217122/1‑71 (MQ=255)                                      ccgACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCt                         >  1:1375448/1‑71 (MQ=255)                                       cgACCTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCt                         <  1:2215004/70‑1 (MQ=255)                                          ccTTTTTCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGcc                     <  1:838474/71‑1 (MQ=255)                                             ttttCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGcc                                            >  1:1098749/1‑45 (MQ=255)                                             ttttCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCtt                        >  1:1348843/1‑65 (MQ=255)                                             ttttCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctct                  >  1:819337/1‑71 (MQ=255)                                              tttCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctc                   <  1:732940/69‑1 (MQ=255)                                              tttCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctc                   <  1:532198/69‑1 (MQ=255)                                                tCCCACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCctc                   <  1:1105667/67‑1 (MQ=255)                                                  ccACGGTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCt              >  1:1345518/1‑70 (MQ=255)                                                      ggTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCtt                        >  1:28872/1‑56 (MQ=255)                                                      ggTGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCtt                        >  1:2268971/1‑56 (MQ=255)                                                        tGCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGAcc       <  1:2789943/71‑1 (MQ=255)                                                         gCAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCa            >  1:450398/1‑65 (MQ=255)                                                          cAAAATTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCtt     >  1:794918/1‑71 (MQ=255)                                                           aaaaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCCCGACCttt    >  1:693004/1‑71 (MQ=255)                                                             aaTTTGCATCGTAGTAAAGTTTTGTTGCCCTCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg  >  1:2454932/1‑71 (MQ=255)                                                             aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCtttt   <  1:605807/70‑1 (MQ=255)                                                             aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCtttt   >  1:734540/1‑70 (MQ=255)                                                             aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCt      <  1:2448891/67‑1 (MQ=255)                                                             aaTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg  >  1:496218/1‑71 (MQ=255)                                                             aaTTTGCATCGTAGTAAAGTTTTGTTGCCACCAAc                                      >  1:1290287/1‑35 (MQ=37)                                                              aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCtt     >  1:2474213/1‑67 (MQ=255)                                                              aTTTGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTg  <  1:1371121/70‑1 (MQ=255)                                                                 tGCATCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCt              >  1:896833/1‑55 (MQ=255)                                                                     |                                                               GATATTTTCTTTATCGCCATACCAGATGCGTTCCGTTCCGACCTTTTTCCCACGGTGCAAAATTTGCACCGTAGTAAAGTTTTGTTGCCCCCAACGCAGTAATTTTCTTGCCTCTTCCTCACGACCTTTTG  >  minE/1302160‑1302290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑