Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	144,272	C→A	G194V (GGC→GTC)	yadB ←	glutamyl‑Q tRNA(Asp) synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	144,272	0	C	A	100.0%	200.4 / NA	68	G194V (GGC→GTC)	yadB	glutamyl‑Q tRNA(Asp) synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/36);  total (32/36)

GCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGCGATGAATGA  >  minE/144204‑144338                                                                     |                                                                   gCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGacc                                                                  >  1:1266221/1‑71 (MQ=255)  cTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCt                                                                 >  1:1018427/1‑71 (MQ=255)     gTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTgg                                                               >  1:2660840/1‑70 (MQ=255)      tACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGaa                                                             <  1:2880919/71‑1 (MQ=255)      tACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGaa                                                             <  1:278368/71‑1 (MQ=255)        cAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAAt                                                           >  1:953725/1‑71 (MQ=255)           cGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGat                                                        >  1:1131285/1‑71 (MQ=255)            gagaTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGa                                                              <  1:1120523/64‑1 (MQ=255)             agaTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatca                                                      >  1:1031024/1‑71 (MQ=255)             agaTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatca                                                      >  1:2093407/1‑71 (MQ=255)               aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCt                                                                 <  1:973575/58‑1 (MQ=255)               aTTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATTatcatc                                                    <  1:5551/71‑1 (MQ=255)                     cTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatcatc                                                    <  1:92537/65‑1 (MQ=255)                     cTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACcac                                              <  1:2790938/71‑1 (MQ=255)                     cTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACca                                               >  1:963480/1‑70 (MQ=255)                       tACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGacc                                                                  <  1:2633310/49‑1 (MQ=255)                       tACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACcaca                                             >  1:2952820/1‑70 (MQ=255)                        aCTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCaa                                                  >  1:2573530/1‑64 (MQ=255)                        aCTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCacaa                                            <  1:1040883/70‑1 (MQ=255)                         cTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATCATCATCAACCacaac                                           <  1:1375757/70‑1 (MQ=255)                          tGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTCTAACGACCTGGAAATGATCATCAACCACAACAg                                         >  1:2783663/1‑71 (MQ=255)                            ttGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGatc                                                       >  1:1496327/1‑55 (MQ=255)                            ttGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGcc                                       <  1:1527751/71‑1 (MQ=255)                            ttGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGc                                        >  1:1627899/1‑70 (MQ=255)                            ttGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGc                                        <  1:227199/70‑1 (MQ=255)                            gttgTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGc                                        >  1:2321027/4‑70 (MQ=255)                               gTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCatg                                    <  1:2251402/71‑3 (MQ=255)                               gTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAg                                     <  1:880364/70‑1 (MQ=255)                               gTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAg                                     <  1:74481/70‑1 (MQ=255)                                ttCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGt                                   >  1:1227839/1‑71 (MQ=255)                                 tCAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGt                                   >  1:1115980/1‑70 (MQ=255)                                  cAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGtt                                  <  1:1644759/70‑1 (MQ=255)                                  cAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTg                                 >  1:544909/1‑71 (MQ=255)                                  cAATCAGATCAGCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTg                                 >  1:1655134/1‑71 (MQ=255)                                   aaTCAGATCAGCCCCACGCACTATTTCTGTAACGACCTg                                                                <  1:2919323/39‑1 (MQ=255)                                    atcagatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTa                               <  1:1406129/71‑1 (MQ=255)                                     tcagatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGtt                                  <  1:1143483/67‑1 (MQ=255)                                      cagatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAgg                             >  1:673886/1‑71 (MQ=255)                                      cagatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAgg                             >  1:1231351/1‑71 (MQ=255)                                       agatcagCCCCACGCACTATTTCTGTAGCGACCTGGAAATGATCATCAACCACAACAGCCAGGt                                   >  1:1802544/1‑64 (MQ=255)                                       agatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAgg                             <  1:2284275/70‑1 (MQ=255)                                        gatcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCg                           <  1:554550/71‑1 (MQ=255)                                          tcagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCg                           <  1:242720/69‑1 (MQ=255)                                           cagCCCCACGCACTATTTCTGTAACGACCTGGAAATg                                                          >  1:2778769/1‑37 (MQ=255)                                           cagCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGGTGTAGGCGaa                         <  1:251328/70‑1 (MQ=255)                                            agCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGaaca                       <  1:1238822/71‑1 (MQ=255)                                            agCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGaaca                       <  1:1226474/71‑1 (MQ=255)                                            agCCCCACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGaaca                       <  1:370575/71‑1 (MQ=255)                                               cccACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAAcc                    <  1:2665968/71‑1 (MQ=255)                                                ccACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAAcc                    >  1:2051920/1‑70 (MQ=255)                                                 cACGCACTATTTCTGTAACGACCTGGAAATGATCATCAACcaca                                             <  1:509960/44‑1 (MQ=255)                                                         aTTTCTGTAACGACCTGGAAATGATCATCAACcaca                                             <  1:1121793/36‑1 (MQ=255)                                                         aTTTCTGTAACGACCTGGAAATGATCATCAACCacaa                                            >  1:1756719/1‑37 (MQ=255)                                                         aTTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                            <  1:2183440/53‑1 (MQ=255)                                                         aTTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGc                            <  1:471932/53‑1 (MQ=255)                                                         aTTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGaacaa                      >  1:1094573/1‑59 (MQ=255)                                                         aTTTCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGaacaa                      >  1:1570176/1‑59 (MQ=255)                                                          tttCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGc           <  1:2832895/69‑1 (MQ=255)                                                          tttCTGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGCGa         >  1:1054498/1‑71 (MQ=255)                                                           ttCTGTAACGACCTGCAAATGATCATCAACCacaac                                           >  1:1733197/1‑36 (MQ=37)                                                              tGTAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGCGatgaa     >  1:2325157/1‑71 (MQ=255)                                                                tAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCTAACAACCCATCACGGCGatga      <  1:1435279/68‑1 (MQ=255)                                                                tAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGCGatgaat    <  1:1889504/70‑1 (MQ=255)                                                                tAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGCGa         <  1:2736165/65‑1 (MQ=255)                                                                tAACGACCTGGAAATGATCATCAACCACAACAGCCAGGTTCTAGGCGAACAAcc                    >  1:2843193/1‑54 (MQ=255)                                                                 aaCGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCAc              <  1:1643183/59‑1 (MQ=255)                                                                 aaCGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGCGatgaatga  >  1:2171981/1‑71 (MQ=255)                                                                  aCGACCTGGAAATGATCATCAACCACAACAGCCAgg                                    >  1:2796856/1‑36 (MQ=255)                                                                  aCGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGCGatgaatga  >  1:459470/1‑70 (MQ=255)                                                                  aCGACCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGCGatgaatga  <  1:134428/70‑1 (MQ=255)                                                                     |                                                                   GCTGGTACAGCGAGATTTGCCTTACTGTTGGTTCAATCAGATCAGCCCCACGCACTATTTCTGTAACGCCCTGGAAATGATCATCAACCACAACAGCCAGGTTGTAGGCGAACAACCCATCACGGCGATGAATGA  >  minE/144204‑144338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑