Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,509,579	G→A	A42T (GCA→ACA)	ackA →	acetate kinase A and propionate kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,509,579	0	G	A	100.0%	205.9 / NA	69	A42T (GCA→ACA)	ackA	acetate kinase A and propionate kinase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/38);  total (31/38)

TCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCTCAC  >  minE/1509514‑1509644                                                                  |                                                                  tcatcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACa                                                                 >  1:30151/1‑68 (MQ=255) tcatcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACg                                                               >  1:2060124/1‑70 (MQ=255) tcatcGATGAAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACa                                                                 >  1:592566/1‑68 (MQ=255)  cagcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                             >  1:1304540/4‑71 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTTGTTTAGCCGAATGTCTCCACCTGCCCGAAACACGTAt                                                            >  1:200910/1‑71 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACa                                                                 >  1:2833852/1‑66 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                             <  1:2911928/70‑1 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTa                                                             <  1:1764498/70‑1 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                            <  1:1324191/71‑1 (MQ=255)   atcGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTAt                                                            <  1:1487246/71‑1 (MQ=255)       aTGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaa                                                        >  1:714200/1‑71 (MQ=255)           aGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatgga                                                    <  1:833424/71‑1 (MQ=255)             tAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaa                                                   >  1:727057/1‑70 (MQ=255)              aaaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                                 >  1:1381020/1‑71 (MQ=255)               aaTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaa                                                 <  1:932670/70‑1 (MQ=255)                aTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggataatg                                               <  1:476158/71‑1 (MQ=255)                aTGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaatg                                               <  1:628950/71‑1 (MQ=255)                 tGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaatg                                               >  1:482417/1‑70 (MQ=255)                 tGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatggaaaatg                                               >  1:485945/1‑70 (MQ=255)                    tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCa                                                          <  1:2237569/56‑1 (MQ=255)                    tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg                                           <  1:2313102/71‑1 (MQ=255)                    tGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACg                                           <  1:1321734/71‑1 (MQ=255)                      tagagTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACgg                                          <  1:2196961/69‑1 (MQ=255)                      aaGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCaaatgga                                                    <  1:731445/60‑1 (MQ=255)                      aaGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACgg                                          <  1:1701557/70‑1 (MQ=255)                      aaGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACgg                                          >  1:739606/1‑70 (MQ=255)                          gTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                     <  1:1612865/71‑1 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATaa                                    <  1:698692/71‑1 (MQ=255)                           tACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                     <  1:1878721/70‑1 (MQ=255)                             ccTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATaaa                                   >  1:1621851/1‑70 (MQ=255)                             ccTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAAc                                  <  1:1303092/71‑1 (MQ=255)                                ttCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAg                                >  1:76562/1‑70 (MQ=255)                                ttCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAg                                >  1:2458151/1‑70 (MQ=255)                                 tCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACTTATCAAATGGAAAATGGACGGCAATAAACAgg                               <  1:2194275/70‑1 (MQ=255)                                 tCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAgg                               <  1:1921199/70‑1 (MQ=255)                                 tCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAg                                <  1:823925/69‑1 (MQ=255)                                  cTGGTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGAc                                            >  1:908717/1‑56 (MQ=255)                                    ggTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCa                                                          <  1:2443085/40‑1 (MQ=255)                                     gTTTAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGaa                             <  1:2741270/68‑1 (MQ=255)                                       ttAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCa                                        >  1:2287579/1‑55 (MQ=255)                                        tAGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCt                       >  1:1426327/1‑71 (MQ=255)                                         aGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCt                       <  1:102117/70‑1 (MQ=255)                                         aGCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCt                       <  1:1619107/70‑1 (MQ=255)                                          gCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCttt                     <  1:2925281/71‑1 (MQ=255)                                          gCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCttt                     <  1:1674586/71‑1 (MQ=255)                                          gCCGAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCttt                     <  1:725320/71‑1 (MQ=255)                                             gAATGTTTCCACCTGCCCGAAACACGTATCaaatggaa                                                   >  1:329264/1‑38 (MQ=255)                                             gAATGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAgg                  >  1:694023/1‑71 (MQ=255)                                              aaTGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAg                                <  1:1812765/56‑1 (MQ=255)                                               aTGTTTCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTg                <  1:330829/71‑1 (MQ=255)                                                  tttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACa                                 >  1:2039074/1‑51 (MQ=255)                                                  tttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACa                                 >  1:1891875/1‑51 (MQ=255)                                                  tttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCa              <  1:1903566/70‑1 (MQ=255)                                                   ttCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAg             <  1:804336/70‑1 (MQ=255)                                                    tCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAAc                                  >  1:913671/1‑48 (MQ=255)                                                    tCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAAc                                  >  1:2543699/1‑48 (MQ=255)                                                    tCCACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAgg            >  1:7564/1‑70 (MQ=255)                                                     ccACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAg                   <  1:961732/62‑1 (MQ=255)                                                     ccACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAg                   <  1:912961/62‑1 (MQ=255)                                                      cACCTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGgcgc         >  1:2123750/1‑71 (MQ=255)                                                         cTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAAc                                  >  1:1687326/1‑43 (MQ=255)                                                         cTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGgcgc         <  1:1998882/68‑1 (MQ=255)                                                         cTGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGcgccgc      <  1:2840711/71‑1 (MQ=255)                                                          tGCCCGAAACACGTATCAAATGGAAAATGGACGGCAATa                                     >  1:2334407/1‑39 (MQ=255)                                                             ccGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCTcac  <  1:2241508/71‑1 (MQ=255)                                                             ccGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCTca   >  1:2832146/1‑70 (MQ=255)                                                             ccGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCTca   >  1:494061/1‑70 (MQ=255)                                                              cGAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCTca   >  1:976570/1‑69 (MQ=255)                                                               gAAACACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCa              <  1:1311431/57‑1 (MQ=255)                                                                  |                                                                  TCATCGATGCAGTAAATGGTGAAGAGTACCTTTCTGGTTTAGCCGAATGTTTCCACCTGCCCGAAGCACGTATCAAATGGAAAATGGACGGCAATAAACAGGAAGCGGCTTTAGGTGCAGGCGCCGCTCAC  >  minE/1509514‑1509644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑