| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,511,849 | T→C | W373R (TGG→CGG) | pta → | phosphate acetyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,511,849 | 0 | T | C | 98.7% | 261.6 / ‑6.7 | 76 | W373R (TGG→CGG) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (37/38); minor base G (0/1); total (37/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTAT > minE/1511781‑1511914 | ttCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCgg < 1:303594/71‑1 (MQ=255) tCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGa > 1:1105170/1‑71 (MQ=255) tCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGa > 1:2527968/1‑71 (MQ=255) ccGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGAt > 1:2294940/1‑71 (MQ=255) cGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGAt < 1:2926962/70‑1 (MQ=255) cGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGAt < 1:1192489/70‑1 (MQ=255) cGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATc > 1:2155045/1‑71 (MQ=255) cGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATc < 1:1630592/71‑1 (MQ=255) ggTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCg < 1:2113817/71‑1 (MQ=255) gTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGa < 1:2406441/71‑1 (MQ=255) ttGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGaa < 1:1920306/71‑1 (MQ=255) aCGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAAtct < 1:2359487/71‑1 (MQ=255) cGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGa > 1:2670294/1‑62 (MQ=255) gATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAAtct > 1:2367926/1‑69 (MQ=255) gATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATctct < 1:1379284/71‑1 (MQ=255) aTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATctct > 1:2234523/1‑70 (MQ=255) aTCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTg < 1:2368266/71‑1 (MQ=255) cACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGa < 1:1418022/70‑1 (MQ=255) cACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGa > 1:1655540/1‑70 (MQ=255) cACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGa > 1:2652185/1‑70 (MQ=255) cACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGAc > 1:992724/1‑71 (MQ=255) aCGAACGTATCGAGAAAGTTCAGGAATACGTTGCCAACTACATCAACGCTGACCGGATCGa < 1:1215893/61‑1 (MQ=255) cGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTg < 1:2416089/71‑1 (MQ=255) aaCGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGcc < 1:684403/71‑1 (MQ=255) aaCGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGc > 1:45596/1‑70 (MQ=255) aaCGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGc > 1:276335/1‑70 (MQ=255) cGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCAc < 1:1060880/71‑1 (MQ=255) cGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCAc < 1:2706761/71‑1 (MQ=255) gTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATctct < 1:1714833/61‑1 (MQ=255) tATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACtt < 1:2467426/71‑1 (MQ=255) cGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTTCCACTTCt < 1:1581852/70‑1 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTg < 1:245976/70‑1 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTg < 1:2369888/70‑1 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTg < 1:1546550/70‑1 (MQ=255) gagaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTg < 1:2506898/70‑1 (MQ=255) agaAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAg < 1:2126774/71‑1 (MQ=255) aaaGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATctct < 1:2435877/53‑1 (MQ=255) aaaGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAgcg > 1:909939/1‑71 (MQ=255) aaaGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAgcg < 1:3051152/71‑1 (MQ=255) aGTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAgcg > 1:2262517/1‑69 (MQ=255) gTTCAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACAGCCACTTCTg < 1:1013958/64‑1 (MQ=255) cAGGAATACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcg > 1:1108384/1‑71 (MQ=255) ggAATACGTTGCTAACTACATCAACGCTGACGGGATCGAATctct < 1:1675000/45‑1 (MQ=255) aaTACGTTGCTAACTACATCAACGCTGACCGGATCTAATCTCTGACTGCCACTTCTGAGCGCAGCcgtcgt < 1:2306408/71‑1 (MQ=255) aTACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgtcgt > 1:1731799/1‑70 (MQ=255) aTACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCgtcgtc > 1:1961658/1‑71 (MQ=255) tACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCa < 1:1380692/61‑1 (MQ=255) tACGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtct > 1:1668116/1‑71 (MQ=255) tcGTTGCTAACTTCATCATCGCTGACCGGATCGAATCTCTGACTGCCACTTCt < 1:730435/52‑1 (MQ=255) cGTTGCTAACTACATCAACGCTGACCGGATCGAAt > 1:1876984/1‑35 (MQ=255) cGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgt > 1:868738/1‑71 (MQ=255) cGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctgt < 1:2866054/71‑1 (MQ=255) cGTTGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCgtctg > 1:2087752/1‑70 (MQ=255) tGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGa > 1:1323059/1‑51 (MQ=255) tGCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGtctc > 1:338600/1‑71 (MQ=255) gCTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGtctc < 1:29414/70‑1 (MQ=255) cTAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCg > 1:855685/1‑71 (MQ=255) tAACTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAgcgc < 1:2368132/52‑1 (MQ=255) aCTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAgcgc > 1:1892823/1‑50 (MQ=255) aCTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAgcg > 1:2661452/1‑49 (MQ=255) aCTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCgtcgtc > 1:702959/1‑60 (MQ=255) aCTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGc < 1:534550/69‑1 (MQ=255) aCTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGc < 1:253206/69‑1 (MQ=255) cTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGcc > 1:1080964/1‑69 (MQ=255) cTACATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTg < 1:2215978/71‑1 (MQ=255) cATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCg < 1:757034/70‑1 (MQ=255) cATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGt > 1:1882942/1‑71 (MQ=255) cATCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGt > 1:270265/1‑71 (MQ=255) tCAACGCTGACCGGATCGAATCTCTGACTGCCACtt > 1:1572362/1‑36 (MQ=255) tCAACGCTGACCGGATCGAATCTCTGACTGCCACtt > 1:1829264/1‑36 (MQ=255) tCAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCcgtcgt > 1:75838/1‑53 (MQ=255) cAACGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTcc > 1:2501760/1‑71 (MQ=255) aCGCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGt < 1:905532/71‑1 (MQ=255) gCTGACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTa > 1:2079845/1‑71 (MQ=255) gACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTAt > 1:203513/1‑69 (MQ=255) gACCGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTAt > 1:2030872/1‑69 (MQ=255) | TTCCGGTTGACGATCACGAACGTATCGAGAAAGTTCAGGAATACGTTGCTAACTACATCAACGCTGACTGGATCGAATCTCTGACTGCCACTTCTGAGCGCAGCCGTCGTCTGTCTCCGCCTGCGTTCCGTTAT > minE/1511781‑1511914 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |