Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,536,834	C→T	M1I (ATG→ATA) †	yfcR ←	predicted fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,536,834	0	C	T	100.0%	158.4 / NA	53	M1I (ATG→ATA) †	yfcR	predicted fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/17);  total (36/17)

AAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGG  >  minE/1536766‑1536901                                                                     |                                                                    aaaCTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGttat                                                                   >  1:1035293/1‑71 (MQ=255)  aaCTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGttat                                                                   >  1:2720072/1‑70 (MQ=255)    cTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGttatca                                                                 <  1:1789514/70‑1 (MQ=255)    cTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAg                                                                >  1:2379192/1‑71 (MQ=255)      aCAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGcc                                                              >  1:523862/1‑71 (MQ=255)      aCAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGcc                                                              >  1:2784691/1‑71 (MQ=255)         gACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCttt                                                           <  1:841409/71‑1 (MQ=255)         gACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCtt                                                            <  1:1372522/70‑1 (MQ=255)            acTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCg                                                        >  1:1272490/1‑71 (MQ=255)            acTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCg                                                        >  1:2702769/1‑71 (MQ=255)              tGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgc                                                      >  1:1741413/1‑71 (MQ=255)                cATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctc                                                    >  1:2758318/1‑71 (MQ=255)                  tACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                                  <  1:463127/71‑1 (MQ=255)                   aCCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTg                                                          >  1:37361/1‑62 (MQ=255)                   aCCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCg                                                        >  1:1915897/1‑64 (MQ=255)                    ccAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctgct                                                <  1:1878436/71‑1 (MQ=255)                     cagcagCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctgctc                                               >  1:1985879/1‑71 (MQ=255)                     cagcagCCCTGCGCCCACTACAAACTTTT‑ACTCATAACTCCCCCCGTTATCAGCCTTTGCGgctctg                                                  >  1:2337665/1‑67 (MQ=255)                       gcagcCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCtt                                             >  1:2306503/1‑71 (MQ=255)                       gcagcCCTGCGCCCACTACAAACTTTTGACTCATAACACCCCCCGTTATCAGCCTTTGCGGCTCTGCTCtt                                             >  1:809271/1‑71 (MQ=255)                         agcCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCa                                           >  1:1440510/1‑71 (MQ=255)                               gcgcCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAcc                                     >  1:831140/1‑71 (MQ=255)                                cgcCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                                      <  1:2959428/69‑1 (MQ=255)                                cgcCCACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCt                                    <  1:613909/71‑1 (MQ=255)                                   ccACTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGc                                 <  1:1042980/71‑1 (MQ=255)                                     aCTACAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAg                               >  1:1893240/1‑71 (MQ=255)                                         cAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCt                             <  1:902010/69‑1 (MQ=255)                                         cAAACTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGt                           >  1:704884/1‑71 (MQ=255)                                           aaCTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTg                         <  1:1858213/71‑1 (MQ=255)                                            aCTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGc                        <  1:780684/71‑1 (MQ=255)                                             cTTTTGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGcc                       <  1:1049072/71‑1 (MQ=255)                                              ttttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCa                      >  1:1231013/1‑71 (MQ=255)                                                ttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCAtt                    >  1:1892466/1‑71 (MQ=255)                                                ttGACTCATAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCAtt                    >  1:330118/1‑71 (MQ=255)                                                       aTAACTCCCCCCGTTATCAGCCTTTGCGgctctgctc                                               <  1:48782/37‑1 (MQ=255)                                                       aTAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTgg             <  1:2839753/71‑1 (MQ=255)                                                       aTAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTg              >  1:612056/1‑70 (MQ=255)                                                       aTAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTg              >  1:2164190/1‑70 (MQ=255)                                                       aTAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTg              >  1:287618/1‑70 (MQ=255)                                                        tAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCt                                    >  1:2093836/1‑47 (MQ=255)                                                        tAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGtt                          >  1:1442154/1‑57 (MQ=255)                                                        tAACTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGga            <  1:117859/71‑1 (MQ=255)                                                         aaCTCCCCCCGTTATCAGCCTTTGCGgctctgctc                                               >  1:2488638/1‑35 (MQ=255)                                                         aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                                      >  1:2752924/1‑44 (MQ=255)                                                         aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                                      >  1:2749365/1‑44 (MQ=255)                                                         aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                                      >  1:1459615/1‑44 (MQ=255)                                                         aaCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTgg             >  1:2546155/1‑69 (MQ=255)                                                          aCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCa                 <  1:291919/64‑1 (MQ=255)                                                          aCTCCCCCCGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTgg             >  1:779218/1‑68 (MQ=255)                                                              cccccGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCAc                                      >  1:324298/1‑39 (MQ=255)                                                              cccccGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGgaagaa        <  1:786441/69‑1 (MQ=255)                                                              cccccGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGgaagaa        <  1:27203/69‑1 (MQ=255)                                                                cccGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAg     >  1:2025531/1‑70 (MQ=255)                                                                cccGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGc    >  1:2271493/1‑71 (MQ=255)                                                                cccGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGc    >  1:942997/1‑71 (MQ=255)                                                                  cGTTATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCgg  >  1:2652753/1‑71 (MQ=255)                                                                     |                                                                    AAACTACAGACACTGCATACCAGCAGCCCTGCGCCCACTACAAACTTTTGACTCATAACTCCCCCCGTCATCAGCCTTTGCGGCTCTGCTCTTCATCCACCTGGCAGCTGTTGCCATTGCACTGGAAGAACAGCGG  >  minE/1536766‑1536901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑