| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,591,415 | G→T | K203N (AAG→AAT) | yfeS → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,591,415 | 0 | G | T | 98.2% | 158.3 / ‑3.6 | 55 | K203N (AAG→AAT) | yfeS | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (23/31); total (23/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAG > minE/1591356‑1591481 | tACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCaa > 1:3042096/1‑71 (MQ=255)tACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCaa > 1:2472876/1‑71 (MQ=255) cGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAAt > 1:1588715/1‑70 (MQ=255) cTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCAc > 1:1079883/1‑69 (MQ=255) tGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAg > 1:1174246/1‑71 (MQ=255) aTTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAgc < 1:1601716/69‑1 (MQ=255) ggTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAgc > 1:106730/1‑65 (MQ=255) tCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGtcatc < 1:2330334/71‑1 (MQ=255) tCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGtcat > 1:1100386/1‑70 (MQ=255) aGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAatat > 1:102929/1‑43 (MQ=255) aGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGtcatca > 1:2089805/1‑70 (MQ=255) ttAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCtt < 1:2850477/71‑1 (MQ=255) tAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTa > 1:2270493/1‑71 (MQ=255) aaaaGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGcat > 1:2933962/2‑68 (MQ=255) aaaGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTa < 1:646976/70‑1 (MQ=255) aaaGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTa < 1:2546270/70‑1 (MQ=255) aaaGTCATGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTa < 1:2779084/70‑1 (MQ=255) gTCATGGGTAAAATCTCCCATAAACTTAAAAatat > 1:2865133/1‑35 (MQ=255) aTGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCt > 1:2261093/1‑62 (MQ=255) aTGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGc < 1:1186060/68‑1 (MQ=255) aTGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCaaa < 1:2585721/71‑1 (MQ=255) aTGGGTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCaa > 1:142248/1‑70 (MQ=255) ggTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTa < 1:2715510/45‑1 (MQ=255) ggTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTa < 1:2828138/45‑1 (MQ=255) ggTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTa < 1:114502/45‑1 (MQ=255) ggTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAAt < 1:2121851/71‑1 (MQ=255) gTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTa < 1:734439/44‑1 (MQ=255) gTAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAAtt < 1:2704798/71‑1 (MQ=255) tAAAATCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCtt < 1:1767549/58‑1 (MQ=255) aaaaTCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAAttct > 1:1930439/1‑71 (MQ=255) aaaTCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAAttctt > 1:902088/1‑71 (MQ=255) aaaTCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAAttct < 1:608253/70‑1 (MQ=255) aaaTCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAAttct < 1:611769/70‑1 (MQ=255) aaTCTCCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAAttcttc < 1:1826004/71‑1 (MQ=255) tcCCATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAAttcttc < 1:98702/67‑1 (MQ=255) ccATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAAt < 1:545244/68‑1 (MQ=255) ccATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAAt < 1:929364/68‑1 (MQ=255) ccATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAAt < 1:2353562/68‑1 (MQ=255) ccATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAAt < 1:1123477/68‑1 (MQ=255) ccATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATggg < 1:2822895/71‑1 (MQ=255) ccATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATggg > 1:2570542/1‑71 (MQ=255) ccATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATggg > 1:1561292/1‑71 (MQ=255) cATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGtcat > 1:2743022/1‑42 (MQ=255) cATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATgggg > 1:2459427/1‑71 (MQ=255) cATAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATggg > 1:1123557/1‑70 (MQ=255) aTAAACTTAAAAATATGGGACTCAATGCACTAGCGCGt < 1:3047187/38‑1 (MQ=255) tAAACTTAAAAATATGGGACTCAATGCACTAGCGCGtc < 1:1045840/38‑1 (MQ=255) tAAACTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTa < 1:1904426/47‑1 (MQ=255) ataCTTAAAAAGATGGGACTCAATGCACTAGCGCGTCTTAAGCTTa < 1:25735/44‑1 (MQ=255) aaaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAAtt > 1:2969358/1‑57 (MQ=255) aaaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAAt > 1:2482766/1‑64 (MQ=255) aaCTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCt > 1:1101410/1‑43 (MQ=255) cTTAAAAATATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACgg < 1:194531/71‑1 (MQ=255) aaaaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTc < 1:1856938/70‑1 (MQ=255) aaaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGt < 1:1829063/68‑1 (MQ=255) aaaTATGGGACTCAATGCACTAGCGCGTCATCAGc > 1:3023474/1‑35 (MQ=255) aaaTATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAg < 1:2082720/70‑1 (MQ=255) | TACGCAACTGCATTCGGTCAGATTAAAGTCATGGGTAAAATCTCCCATAAACTTAAAAAGATGGGACTCAATGCACTAGCGCGTCATCAGCTTACCGCAAAAATTCTTCAATGGGGTGACGGTCAG > minE/1591356‑1591481 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |