Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,643,860	A→G	R9R (CGT→CGC)	purC ←	phosphoribosylaminoimidazole‑succinocarboxamide synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,643,860	0	A	G	100.0%	191.3 / NA	54	R9R (CGT→CGC)	purC	phosphoribosylaminoimidazole‑succinocarboxamide synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/25);  total (29/25)

GTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATCTT  >  minE/1643792‑1643926                                                                     |                                                                   gTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACcgcg                                                                  <  1:1012086/71‑1 (MQ=255)     cATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATa                                                               >  1:741373/1‑70 (MQ=255)      aTTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATAc                                                              <  1:2997367/70‑1 (MQ=255)      aTTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACa                                                             <  1:1619643/71‑1 (MQ=255)       ttGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGa                                                                 <  1:610751/66‑1 (MQ=255)             aaTTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGa                                                                 >  1:2200295/1‑60 (MQ=255)             aaTTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAgc                                                      >  1:2512209/1‑71 (MQ=255)                tCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAgcttg                                                   >  1:2785375/1‑71 (MQ=255)                 cGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAgcttgc                                                  >  1:323358/1‑71 (MQ=255)                  gAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAgcttgc                                                  <  1:2474092/70‑1 (MQ=255)                  aaGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAgcttgct                                                 <  1:1519337/70‑1 (MQ=255)                   aGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAgcttgctt                                                <  1:2360416/71‑1 (MQ=255)                    gCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCttt                                               >  1:1211567/1‑71 (MQ=255)                     cACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCttt                                               <  1:2404991/70‑1 (MQ=255)                       ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                            <  1:753938/71‑1 (MQ=255)                       ccAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGc                                            <  1:1918214/71‑1 (MQ=255)                          aCAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATc                                         >  1:1542793/1‑71 (MQ=255)                            aGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCtt                                       <  1:2784162/71‑1 (MQ=255)                             ggTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCttt                                      <  1:735046/71‑1 (MQ=255)                              gTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTa                                     <  1:2660786/71‑1 (MQ=255)                                 cGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCa                                           >  1:1412927/1‑62 (MQ=255)                                 cGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCAt                                          >  1:326555/1‑63 (MQ=255)                                 cGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCa                                  <  1:2310326/71‑1 (MQ=255)                                   ggTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCtt                                       >  1:2285927/1‑64 (MQ=255)                                   ggTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                    >  1:668526/1‑67 (MQ=255)                                   ggTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTAt                                    >  1:1600039/1‑67 (MQ=255)                                    gTTTTCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAgcttgctt                                                <  1:1250091/54‑1 (MQ=255)                                     ttttCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAgc                                                      >  1:2066173/1‑47 (MQ=255)                                      tttCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCt                             >  1:2112912/1‑71 (MQ=255)                                       ttCCGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCAc                                 >  1:1353054/1‑66 (MQ=255)                                         ccGTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCa                                           >  1:1883270/1‑54 (MQ=255)                                           gTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTggg                          >  1:1946291/1‑69 (MQ=255)                                           gTGCTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGgt                         >  1:1999504/1‑70 (MQ=255)                                              cTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGtgtg                      <  1:2992601/70‑1 (MQ=255)                                              cTGTATACGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGtgtg                      >  1:946043/1‑70 (MQ=255)                                                 tataCGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTggg                          <  1:2245982/63‑1 (MQ=255)                                                    aCGGTTTTCGCTTTAACGCGATACAACTCAgcttgctt                                                <  1:142143/38‑1 (MQ=38)                                                     cGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCtttt                                              >  1:1741319/1‑39 (MQ=255)                                                     cGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCAc                                 >  1:702854/1‑52 (MQ=255)                                                     cGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACg              >  1:2088821/1‑71 (MQ=255)                                                     cGGTTTTCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACg              >  1:1437765/1‑71 (MQ=255)                                                        atttCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGtt            <  1:1991738/69‑1 (MQ=255)                                                          ttCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTc                               >  1:926075/1‑49 (MQ=255)                                                          ttCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGtttt          >  1:1157157/1‑70 (MQ=255)                                                          ttCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGtttt          >  1:454863/1‑70 (MQ=255)                                                           tCGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTa         >  1:2605243/1‑70 (MQ=255)                                                            cGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTaa        <  1:1593930/70‑1 (MQ=255)                                                            cGCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTaa        <  1:1597580/70‑1 (MQ=255)                                                             gCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTGCTGGGTGTGAATTAACGTTTTaaa       <  1:1078659/70‑1 (MQ=255)                                                             gCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTaaaa      <  1:2744825/71‑1 (MQ=255)                                                             gCTTTACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTaaa       <  1:2865750/70‑1 (MQ=255)                                                                ttACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTaa                >  1:2045946/1‑58 (MQ=255)                                                                 tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATCtt  >  1:1014474/1‑71 (MQ=255)                                                                 tACCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATCt   <  1:208711/70‑1 (MQ=255)                                                                  aCCGCGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATCtt  <  1:137849/70‑1 (MQ=255)                                                                     |                                                                   GTATCATTGCGGAATTCGAGCACCAACAGGTCCGGGTTTTCCGTGCTGTATACGGTTTTCGCTTTACCACGATACAACTCAGCTTGCTTTTGCATCTTTATCACTCCTGGGTGTGAATTAACGTTTTAAAATCTT  >  minE/1643792‑1643926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑