| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,664,529 | G→A | I231I (ATC→ATT) | guaA ← | GMP synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,664,529 | 0 | G | A | 97.4% | 113.4 / ‑4.0 | 39 | I231I (ATC→ATT) | guaA | GMP synthetase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (16/22); total (16/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTTACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGC > minE/1664462‑1664595 | ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt > 1:1179355/1‑70 (MQ=255)ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt > 1:857232/1‑70 (MQ=255)ttttACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAAt > 1:258075/1‑70 (MQ=255) ccGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACttt < 1:1923872/71‑1 (MQ=255) aTAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTAtc > 1:2412393/1‑71 (MQ=255) tAGCGCGGTGCAGCAGCATTGGGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTAtcg < 1:348016/71‑1 (MQ=255) cgGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGcc < 1:2430146/71‑1 (MQ=255) ggTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGcc > 1:437908/1‑70 (MQ=255) agcagcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTg > 1:1469050/1‑71 (MQ=255) gcagcaTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGc < 1:1064735/71‑1 (MQ=255) caTTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTcgc < 1:2720418/71‑1 (MQ=255) ttGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCgg < 1:150049/71‑1 (MQ=255) cGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATg < 1:1410041/71‑1 (MQ=255) ggTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGc < 1:2948953/71‑1 (MQ=255) ggTTACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGc < 1:2028608/71‑1 (MQ=255) ttACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACttt < 1:2215658/43‑1 (MQ=255) ttACGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCgg < 1:1071038/65‑1 (MQ=255) aCGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTAt > 1:1203542/1‑43 (MQ=255) cGGAGGAATCCACACCACCATAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGc < 1:2863151/70‑1 (MQ=255) cGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTAtcgt < 1:147125/45‑1 (MQ=255) cGGAGGAATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGc < 1:2459475/70‑1 (MQ=255) aggaATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTa > 1:2830392/1‑69 (MQ=255) aggaATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACa < 1:284008/71‑1 (MQ=255) ggaATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAg > 1:1711045/1‑71 (MQ=255) gaATCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAg > 1:2944469/1‑70 (MQ=255) aTCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGc < 1:883671/41‑1 (MQ=255) aTCCACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCt < 1:1800125/43‑1 (MQ=255) ccACACCACCAGAGAGGCCGAGAATGACTTTATCGTCGCCTAc > 1:855375/1‑43 (MQ=255) caccacCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgtcg < 1:2348687/71‑1 (MQ=255) caccacCAGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCAtcgag > 1:2460834/1‑68 (MQ=255) ccaccaGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGa > 1:1503923/1‑70 (MQ=255) caccaGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGa > 1:188995/1‑69 (MQ=255) caccaGAGAGGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATa < 1:349521/71‑1 (MQ=255) agagagGCCGAGGATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAAttt < 1:826882/71‑1 (MQ=255) gagGCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGAt > 1:2193929/1‑42 (MQ=255) ggCCGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGGCGATAATTTTCGCt < 1:816818/71‑1 (MQ=255) gCCGAGAATGACTTTATCGTCGCCTACCTGCTcgct < 1:1944171/36‑2 (MQ=39) cGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGa > 1:558919/1‑57 (MQ=255) cGAGAATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGc < 1:1926354/71‑1 (MQ=255) gagaATGACTTTATCGTCGCCTACCTGCTCGCGGATg < 1:443309/37‑1 (MQ=255) | TTTTACCGATAGCGCGGTGCAGCAGCATTGCGGTTACGGAGGAATCCACACCACCAGAGAGGCCGAGGATGACTTTATCGTCGCCTACCTGCTCGCGGATGCGAGCTACAGCATCGTCGATAATTTTCGCTGGC > minE/1664462‑1664595 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |