Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	195,616	C→T	L71L (CTG→CTA)	yaeF ←	predicted lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	195,616	0	C	T	100.0%	106.1 / NA	36	L71L (CTG→CTA)	yaeF	predicted lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/18);  total (18/18)

CGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTT  >  minE/195549‑195683                                                                    |                                                                    cGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAg                                                                   >  1:2907832/1‑70 (MQ=255)  gTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGc                                                                  >  1:190858/1‑70 (MQ=255)      cTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATc                                                             <  1:1630587/71‑1 (MQ=255)      cTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATc                                                             >  1:2392761/1‑71 (MQ=255)      cTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATc                                                             <  1:2326480/71‑1 (MQ=255)           agagGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCgg                                                        >  1:938000/1‑71 (MQ=255)                aaGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGtt                                                     <  1:2658319/69‑1 (MQ=255)                   tGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTgaga                                                <  1:1147986/71‑1 (MQ=255)                     cTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGAt                                               <  1:2995441/70‑1 (MQ=255)                     cTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGAt                                               >  1:2522601/1‑70 (MQ=255)                       gAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAg                                            >  1:413243/1‑71 (MQ=255)                          gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTgag                                                 >  1:2457075/1‑63 (MQ=255)                               ggATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGt                                     >  1:358202/1‑70 (MQ=255)                                  ttCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTAtt                                 >  1:464450/1‑71 (MQ=255)                                   tCCAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTAtt                                 >  1:246069/1‑70 (MQ=255)                                     cAAATGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTAttt                                <  1:1127417/69‑1 (MQ=255)                                       aaTGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCttt                            >  1:1626483/1‑71 (MQ=255)                                        aTGAGGTTACCCCAAGGCTTGAGGAGTATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCtttt                           <  1:2870769/71‑1 (MQ=255)                                        aTGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGc                                        >  1:1334046/1‑58 (MQ=255)                                        aTGAGGTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCtttt                           <  1:1955871/71‑1 (MQ=255)                                             gTTACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTAtt                                 <  1:1819427/60‑1 (MQ=255)                                              ttACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGt                                                      <  1:301896/38‑1 (MQ=255)                                              ttACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTAtt                                 <  1:1503519/59‑1 (MQ=255)                                              ttACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTAtt                                 <  1:2751459/59‑1 (MQ=255)                                               tACCCCAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTattgatt                    >  1:1050283/1‑71 (MQ=255)                                                  cccAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGAt                                               <  1:547440/41‑1 (MQ=255)                                                  cccAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCt                              <  1:1026206/58‑1 (MQ=255)                                                   ccAAGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTc                <  1:2511729/71‑1 (MQ=255)                                                    cAAGGCTAGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTAt                                  >  1:1579057/1‑52 (MQ=255)                                                    cAAGGCGTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCg               >  1:3304/1‑71 (MQ=255)                                                     aaGGCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGAt                                               <  1:66226/38‑1 (MQ=255)                                                       ggCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGt             >  1:1387629/1‑70 (MQ=255)                                                       ggCTTGAGGAGAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGt             >  1:826549/1‑70 (MQ=255)                                                              ggagAATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGc     >  1:1909855/1‑71 (MQ=255)                                                                 gaATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt  <  1:2174558/71‑1 (MQ=255)                                                                 gaATAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCttt  <  1:868666/71‑1 (MQ=255)                                                                    |                                                                    CGTGACTCACAGAGGAAGTGCTGAAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTT  >  minE/195549‑195683

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑