Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,700,462	A→G	L24P (CTA→CCA)	hcaT ←	predicted 3‑phenylpropionic transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,700,462	0	A	G	100.0%	186.4 / NA	53	L24P (CTA→CCA)	hcaT	predicted 3‑phenylpropionic transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/20);  total (33/20)

CCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAA  >  minE/1700395‑1700528                                                                    |                                                                   ccccAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGaa                                                                 <  1:2291812/71‑1 (MQ=255)   ccAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGaaaa                                                               <  1:1789475/71‑1 (MQ=255)   ccAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGaaa                                                                >  1:2873856/1‑70 (MQ=255)   ccAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGaaa                                                                >  1:604165/1‑70 (MQ=255)    cAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGaaaaa                                                              <  1:958344/71‑1 (MQ=255)    cAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGaaaaa                                                              >  1:425958/1‑71 (MQ=255)         aCAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCg                                                         >  1:2915690/1‑71 (MQ=255)         aCAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCg                                                         >  1:126406/1‑71 (MQ=255)                gATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTa                                                   >  1:772761/1‑70 (MQ=255)                  tGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTaaaa                                                <  1:737534/71‑1 (MQ=255)                   ggTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTaa                                                      >  1:977238/1‑64 (MQ=255)                    gTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTaaaa                                                >  1:808826/1‑69 (MQ=255)                      ttCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAg                                             >  1:1953893/1‑70 (MQ=255)                      ttCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAg                                             >  1:1597448/1‑70 (MQ=255)                       tCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAg                                             >  1:1037335/1‑69 (MQ=255)                        cTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTa                                           <  1:2872460/70‑1 (MQ=255)                         tGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGaaaa                                                               >  1:1450079/1‑49 (MQ=255)                            cGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTAtgtg                                       >  1:2832186/1‑70 (MQ=255)                              ttAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTaaaaaa                                              >  1:1123772/1‑61 (MQ=255)                               tAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGaaa                                    >  1:49812/1‑70 (MQ=255)                                aaaCCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGaaa                                    >  1:915488/1‑69 (MQ=255)                                aaaCCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGaaa                                    >  1:2589143/1‑69 (MQ=255)                                  aCCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGc                                >  1:903368/1‑71 (MQ=255)                                    cAATCCCTTTAAGCCAGACGCTCCAGAAAGGTGGaaaaa                                                              >  1:997676/1‑39 (MQ=255)                                     aaTCCCTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGa                             <  1:2209674/71‑1 (MQ=255)                                      aTCCCTTTAAGCCAGACGCTCCAGAAAGGTGGaaaa                                                               >  1:1359520/1‑36 (MQ=255)                                          cTTTAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTaaaa                                                <  1:727126/47‑1 (MQ=255)                                           tttAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAg                                 <  1:1213925/61‑1 (MQ=255)                                            ttAAGCCAGACGCTCCAGAAAGGTGGAAAAATGCCg                                                         >  1:669408/1‑36 (MQ=255)                                                  cAGACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAgc                 <  1:2408915/70‑1 (MQ=255)                                                    gACGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGcgcg              <  1:872131/71‑1 (MQ=255)                                                      cGCTCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg            <  1:317447/71‑1 (MQ=255)                                                         tCCAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGcgcg              <  1:1964366/66‑1 (MQ=255)                                                          ccAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAg                                 <  1:766257/46‑1 (MQ=255)                                                          ccAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg            >  1:2913801/1‑67 (MQ=255)                                                           cAGAAAGGTGTAAAAATGCCGTAACTAAAAAAGTAt                                          >  1:982674/1‑36 (MQ=37)                                                           cAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTAt                                          >  1:486883/1‑36 (MQ=255)                                                           cAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTg       <  1:792887/71‑1 (MQ=255)                                                           cAGAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTg       >  1:1548455/1‑71 (MQ=255)                                                             gAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGc      >  1:2238938/1‑70 (MQ=255)                                                             gAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCa     <  1:933269/71‑1 (MQ=255)                                                             gAAAGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCa     <  1:1012972/71‑1 (MQ=255)                                                              aaaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCa     >  1:1284760/1‑70 (MQ=255)                                                              aaaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCa     >  1:1240692/1‑70 (MQ=255)                                                               aaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAg                                 >  1:1565718/1‑41 (MQ=255)                                                               aaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTg            >  1:2045502/1‑62 (MQ=255)                                                               aaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCaaa   <  1:2814757/71‑1 (MQ=255)                                                               aaGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCaa    >  1:1127486/1‑70 (MQ=255)                                                                aGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAg                            >  1:2466522/1‑45 (MQ=255)                                                                aGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTg       <  1:1692824/66‑1 (MQ=255)                                                                aGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTg       <  1:3017925/66‑1 (MQ=255)                                                                aGGTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCaaaa  >  1:1050309/1‑71 (MQ=255)                                                                 ggTGGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGcc                               <  1:2455088/41‑1 (MQ=255)                                                                    |                                                                   CCCCAATAACAGGCCGATGGTTTCTGGCGTTAAACCAATCCCTTTAAGCCAGACGCTCCAGAAAGGTAGAAAAATGCCGTAACTAAAAAAGTATGTGAAATAGCCGAGCGCCAACCAGCGCGTGGATTGCAAAA  >  minE/1700395‑1700528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑