Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,777,713	T→C	N105D (AAT→GAT)	trmD ←	tRNA (guanine‑1‑)‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,777,713	0	T	C	100.0%	220.2 / NA	62	N105D (AAT→GAT)	trmD	tRNA (guanine‑1‑)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/25);  total (37/25)

ATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTT  >  minE/1777646‑1777779                                                                    |                                                                   aTTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGt                                                                 >  1:793792/1‑71 (MQ=255)        tttGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGt                                                          >  1:1185642/1‑71 (MQ=255)         ttGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGtt                                                         >  1:2455612/1‑71 (MQ=255)          tGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCg                                                                  >  1:2916470/1‑61 (MQ=255)          tGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTc                                                        >  1:2460129/1‑71 (MQ=255)              tCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGtt                                                                <  1:798080/59‑1 (MQ=255)                aCGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGc                                                              >  1:266432/1‑59 (MQ=255)                aCGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGAc                                                  >  1:695141/1‑71 (MQ=255)                 cgcgCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGcc                                                             >  1:2387533/1‑59 (MQ=255)                 cgcgCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGa                                                   >  1:2215477/1‑69 (MQ=255)                   cgcTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCg                                                                  <  1:2888104/52‑1 (MQ=255)                    gcTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGccc                                              >  1:997588/1‑71 (MQ=255)                     cTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                                     >  1:801986/1‑63 (MQ=255)                     cTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCt                                                     >  1:195782/1‑63 (MQ=255)                     cTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCg                                             <  1:420670/71‑1 (MQ=255)                     cTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCg                                             >  1:242172/1‑71 (MQ=255)                     cTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCg                                             <  1:1047668/71‑1 (MQ=255)                      tCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTAGATCCg                                                                  <  1:401/49‑1 (MQ=255)                       cATCTATACCTTCGTAGCGACAGCACACCAGAATCAATTTTTGATCAGTTGc                                                              <  1:952333/52‑1 (MQ=38)                        aTCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGc                                            <  1:783916/69‑1 (MQ=255)                         tCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACg                                                 <  1:1905326/63‑1 (MQ=255)                             taCCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTg                                         <  1:1156812/67‑1 (MQ=255)                             taCCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATc                                      <  1:2192217/70‑1 (MQ=255)                             taCCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATc                                      <  1:2159572/70‑1 (MQ=255)                             taCCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATc                                      <  1:1120825/70‑1 (MQ=255)                               ccTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                                   >  1:217284/1‑71 (MQ=255)                               ccTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                                   >  1:1574151/1‑71 (MQ=255)                                cTTCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCAGCTTGATCAAGc                                  >  1:2018442/1‑71 (MQ=255)                                 ttCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGa                                                   >  1:1843352/1‑53 (MQ=255)                                 ttCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGa                                                   >  1:2586177/1‑53 (MQ=255)                                 ttCGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCt                                 >  1:1422136/1‑71 (MQ=255)                                   cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCtt                                <  1:432670/70‑1 (MQ=255)                                   cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTg                               >  1:2162896/1‑71 (MQ=255)                                   cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTg                               >  1:1482605/1‑71 (MQ=255)                                   cGTAGCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTg                               >  1:1336363/1‑71 (MQ=255)                                       gCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCaa                                    >  1:2718220/1‑62 (MQ=255)                                       gCGACCGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGt                            >  1:2997685/1‑70 (MQ=255)                                           ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCtt                       <  1:801632/71‑2 (MQ=255)                                           ccGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCtg                       <  1:2522311/71‑1 (MQ=255)                                            cGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGAc                                                  >  1:853875/1‑43 (MQ=255)                                            cGCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCtgt                      <  1:2222746/71‑1 (MQ=255)                                             gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCtgtg                     >  1:2456095/1‑71 (MQ=255)                                             gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCtgtg                     <  1:332502/71‑1 (MQ=255)                                             gCACACCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCtgtg                     <  1:2497218/71‑1 (MQ=255)                                              cacaCCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtgg                    >  1:1436376/1‑71 (MQ=255)                                               acacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGa                                        <  1:2558081/50‑1 (MQ=255)                                               acacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggt                   <  1:44106/71‑1 (MQ=255)                                                cacCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTcc                          >  1:383556/1‑63 (MQ=255)                                                 acCAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGa                 <  1:1009431/71‑1 (MQ=255)                                                  ccAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGccc                                              >  1:2569635/1‑41 (MQ=255)                                                   cAGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGa                                        >  1:998519/1‑46 (MQ=255)                                                    aGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                                   <  1:2794579/50‑1 (MQ=255)                                                    aGAATCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAg                                   <  1:1915162/50‑1 (MQ=255)                                                      aaTCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTgtggt                   <  1:961827/64‑1 (MQ=255)                                                       aTCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCtt                                >  1:2004345/1‑50 (MQ=255)                                                        tCAATTTTTGATCCGTTGCCAGTTCGCTGACGCCCg                                             >  1:2481332/1‑36 (MQ=255)                                                          aaTTTTTGATCCGTTGCCAGTTCGCTAACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAAt        >  1:1043371/1‑71 (MQ=255)                                                            tttttGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCa      >  1:2419526/1‑71 (MQ=255)                                                             ttttGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATa           >  1:950827/1‑65 (MQ=255)                                                              tttGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCAcc    >  1:826222/1‑71 (MQ=255)                                                                tGATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATa           >  1:999731/1‑62 (MQ=255)                                                                 gATCCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCtt  <  1:1951317/70‑1 (MQ=255)                                                                    |                                                                   ATTTCGGTTTGGATCACGCGCTCATCTATACCTTCGTAGCGACCGCACACCAGAATCAATTTTTGATTCGTTGCCAGTTCGCTGACGCCCGCTTGATCAAGCTTGCGTCCCTGTGGTGACAGATAAATCACCTT  >  minE/1777646‑1777779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑