Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,784,184	T→C	I223T (ATA→ACA)	yfjB →	NAD kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,784,184	0	T	C	100.0%	307.2 / NA	85	I223T (ATA→ACA)	yfjB	NAD kinase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (50/35);  total (50/35)

CCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGA  >  minE/1784116‑1784250                                                                     |                                                                   ccccTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACaa                                                                  <  1:2426802/71‑1 (MQ=255)   ccTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACaaa                                                                 >  1:707083/1‑70 (MQ=255)   ccTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACaaa                                                                 >  1:1425334/1‑70 (MQ=255)    ctctctGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACaaa                                                                 >  1:1653247/1‑69 (MQ=255)    ctctctGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACaaa                                                                 >  1:1836045/1‑69 (MQ=255)    ctctctGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACaaa                                                                 >  1:519774/1‑69 (MQ=255)      ctctGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAAcag                                                              >  1:2178150/1‑70 (MQ=255)       tctGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAAcag                                                              >  1:940879/1‑69 (MQ=255)       tctGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAAcag                                                              >  1:2174114/1‑69 (MQ=255)       tctGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAAca                                                               >  1:1288398/1‑68 (MQ=255)        ctGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAAcagcag                                                           >  1:1141572/1‑71 (MQ=255)          ggATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAAcagcag                                                           >  1:1504691/1‑69 (MQ=255)           gATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACTACCACTGGTCACAAAcagcagc                                                          <  1:1669384/69‑1 (MQ=255)           gATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAAcagcagcag                                                        <  1:1606759/71‑1 (MQ=255)           gATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAAcagcagcag                                                        >  1:2814414/1‑71 (MQ=255)           gATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAAcagcag                                                           <  1:388511/68‑1 (MQ=255)            aTGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAAcagcagcag                                                        >  1:209796/1‑70 (MQ=255)             tGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAAcagca                                                            <  1:241168/65‑1 (MQ=255)               cGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACg                                                    <  1:1006848/71‑1 (MQ=255)                gATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAAcagcag                                                           <  1:920388/63‑1 (MQ=255)                gATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGa                                                   <  1:1387348/71‑1 (MQ=255)                 aTTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACg                                                    <  1:2236655/69‑1 (MQ=255)                 aTTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGAt                                                  <  1:695476/71‑1 (MQ=255)                   tACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATcc                                                >  1:2267239/1‑71 (MQ=255)                    aCCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCg                                               >  1:3008803/1‑71 (MQ=255)                      ccTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAAcagcag                                                           <  1:207788/57‑1 (MQ=255)                        tGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAAcagcagca                                                         >  1:655401/1‑57 (MQ=255)                        tGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTg                                           >  1:2672220/1‑71 (MQ=255)                        tGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTg                                           >  1:1152325/1‑71 (MQ=255)                          gTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGc                                          <  1:2428010/70‑1 (MQ=255)                          gTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCg                                         <  1:2429539/71‑1 (MQ=255)                          gTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCg                                         <  1:1306804/71‑1 (MQ=255)                           tGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGt                                        >  1:2943750/1‑71 (MQ=255)                            gCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGtt                                       >  1:1764038/1‑71 (MQ=255)                              ccATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGtttt                                     >  1:1521639/1‑71 (MQ=255)                                aTGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATcc                                                <  1:2295863/58‑1 (MQ=255)                                aTGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATcc                                                <  1:348023/58‑1 (MQ=255)                                aTGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATcc                                                <  1:203622/58‑1 (MQ=255)                                aTGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTc                                   >  1:2671272/1‑71 (MQ=255)                                aTGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTc                                   >  1:1008307/1‑71 (MQ=255)                                aTGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTc                                   <  1:1377227/71‑1 (MQ=255)                                 tGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCt                                            >  1:1129190/1‑61 (MQ=255)                                 tGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCg                                  >  1:1237937/1‑71 (MQ=255)                                 tGTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCAAGATCCGTCTGCGTTTTTCg                                  >  1:1439273/1‑71 (MQ=255)                                  gTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGt                                        <  1:2357918/64‑1 (MQ=255)                                  gTTCCCGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCg                                  >  1:1444403/1‑70 (MQ=255)                                   ttCCCGCATACGTTGTCAGCACGCCCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCg                                  >  1:2268103/1‑69 (MQ=255)                                      ccGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCg                             <  1:2805240/71‑1 (MQ=255)                                      ccGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCg                             >  1:491125/1‑71 (MQ=255)                                      ccGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCg                             >  1:239107/1‑71 (MQ=255)                                       cGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTc                                   <  1:23901/64‑1 (MQ=255)                                       cGCATACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGc                            >  1:1584454/1‑71 (MQ=255)                                          aTACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCg                          >  1:58333/1‑70 (MQ=255)                                          aTACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCg                          >  1:1667382/1‑70 (MQ=255)                                          aTACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt                         <  1:423991/71‑1 (MQ=255)                                          aTACGTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt                         <  1:2814136/71‑1 (MQ=255)                                              gatGTCAGCACGACCACTGGTCACAAACACCAGCAGCACGATCCGTCTGCGt                                        >  1:761874/3‑52 (MQ=38)                                              gTTGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTaa                       >  1:2733170/1‑69 (MQ=255)                                              gTTGACAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGt                                              >  1:1522992/1‑46 (MQ=39)                                                tGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAc                   >  1:353419/1‑71 (MQ=255)                                                tGTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATAGCCGTAACGAc                   >  1:992045/1‑71 (MQ=255)                                                 gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGtt                                       >  1:2111875/1‑50 (MQ=255)                                                 gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGtt                                       >  1:2403766/1‑50 (MQ=255)                                                 gTCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGAcc                  >  1:1527539/1‑71 (MQ=255)                                                  tCAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCt                 <  1:2417879/71‑1 (MQ=255)                                                   cAGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTg                >  1:117794/1‑71 (MQ=255)                                                    aGCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCg                                  >  1:2022433/1‑52 (MQ=255)                                                     gCACGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt                         <  1:2268046/60‑1 (MQ=255)                                                      cACGACCACTGGTCACAAACAGCAGCAGCACGATCCGt                                              >  1:1772052/1‑38 (MQ=255)                                                       aCGACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTaa                       >  1:622721/1‑60 (MQ=255)                                                         gACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGtttt                                     >  1:1996538/1‑44 (MQ=255)                                                         gACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGc                                 <  1:364223/48‑1 (MQ=255)                                                         gACCACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGc                                 <  1:1688735/48‑1 (MQ=255)                                                           ccACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAg        <  1:1355928/71‑1 (MQ=255)                                                            cACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGt       <  1:1301044/71‑1 (MQ=255)                                                            cACTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGt       <  1:193756/71‑1 (MQ=255)                                                             aCTGGTCACAAACAGCAGCGGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGtt      >  1:1050327/1‑71 (MQ=255)                                                             aCTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGtt      >  1:317570/1‑71 (MQ=255)                                                              cTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGt       <  1:1116149/69‑1 (MQ=255)                                                              cTGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTg     >  1:1731242/1‑71 (MQ=255)                                                               tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGa              <  1:1477784/61‑1 (MQ=255)                                                               tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATc          <  1:2661059/65‑1 (MQ=255)                                                               tGGTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc    >  1:184301/1‑71 (MQ=255)                                                                 gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGa  <  1:1629276/71‑1 (MQ=255)                                                                 gTCACAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGa  <  1:903718/71‑1 (MQ=255)                                                                     |                                                                   CCCCTCTCTGGATGCGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGA  >  minE/1784116‑1784250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑