Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	1,833,531	C→T	R92C (CGC→TGC)	ygbL →	predicted class II aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	1,833,531	0	C	T	100.0%	185.5 / NA	63	R92C (CGC→TGC)	ygbL	predicted class II aldolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/36);  total (27/36)

AATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCT  >  minE/1833463‑1833595                                                                     |                                                                 aaTGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTTGCGCTGTATCGCAACAATCCgtgc                                                                <  1:1551488/71‑1 (MQ=255)  aTGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCt                                                               >  1:840155/1‑71 (MQ=255)  aTGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCt                                                               >  1:650226/1‑71 (MQ=255)  aTGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCt                                                               >  1:968130/1‑71 (MQ=255)   tGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTg                                                              <  1:7332/71‑1 (MQ=255)   tGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCAGTGCTg                                                              <  1:3563/71‑1 (MQ=255)     gTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCAGTGCTg                                                              <  1:1292329/69‑1 (MQ=255)         aGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGc                                                        <  1:2037184/71‑1 (MQ=255)             gtgACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtg                                                    <  1:531061/71‑1 (MQ=255)              tgACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtgg                                                   >  1:1988609/1‑71 (MQ=255)                aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtggt                                                  <  1:2816957/70‑1 (MQ=255)                aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                                 >  1:1608770/1‑71 (MQ=255)                aCAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                                 >  1:403787/1‑71 (MQ=255)                 cAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGgtggtg                                                 >  1:1441671/1‑70 (MQ=255)                  aaaCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCa                                               >  1:284090/1‑71 (MQ=255)                  aaaCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCa                                               <  1:1339374/71‑1 (MQ=255)                   aaCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCa                                               <  1:2869690/70‑1 (MQ=255)                   aaCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCAt                                              <  1:1153373/71‑1 (MQ=255)                    aCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCAtt                                             >  1:902177/1‑71 (MQ=255)                    aCCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCAtt                                             <  1:1572889/71‑1 (MQ=255)                     cccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTTGTGCAttt                                            <  1:1177534/71‑1 (MQ=255)                      ccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtggt                                                  <  1:579525/64‑1 (MQ=255)                      ccTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTg                                           <  1:1852501/71‑1 (MQ=255)                       cTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCggtg                                                    <  1:629322/61‑1 (MQ=255)                        tCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgca                                         >  1:900936/1‑71 (MQ=255)                         cGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgca                                         >  1:2726324/1‑70 (MQ=255)                          gAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgca                                         <  1:756290/69‑1 (MQ=255)                          gAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgca                                         <  1:999381/69‑1 (MQ=255)                              gagGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTg                                           <  1:230146/63‑1 (MQ=255)                               agGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgcacagcaca                                  <  1:413266/71‑1 (MQ=255)                               agGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTgcac                                        >  1:238669/1‑65 (MQ=255)                                  tGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATgg                               <  1:2255018/71‑1 (MQ=255)                                  tGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATgg                               <  1:2831479/71‑1 (MQ=255)                                  tGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATgg                               <  1:2043411/71‑1 (MQ=255)                                  tGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATgg                               <  1:1803265/71‑1 (MQ=255)                                   gCTCTTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCa                                               >  1:697684/1‑54 (MQ=255)                                      ctTTCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGa                           >  1:281516/1‑71 (MQ=255)                                       tttCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGa                           >  1:1353843/1‑70 (MQ=255)                                         tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGa                           <  1:1206103/68‑1 (MQ=255)                                         tCATCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGAc                          <  1:2227861/69‑1 (MQ=255)                                            tCTGGCGCTGTATCGCAACAATCCGTGCTGTAAAGCg                                                       >  1:156070/1‑37 (MQ=255)                                                gcgcTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCtt                   <  1:2933878/69‑1 (MQ=255)                                                 cgcTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcc                <  1:2639802/71‑1 (MQ=255)                                                  gcTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcct               <  1:835511/71‑1 (MQ=255)                                                  gcTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcct               <  1:712143/71‑1 (MQ=255)                                                  gcTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTc                 <  1:1544007/69‑1 (MQ=255)                                                   cTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcctg              <  1:175543/71‑1 (MQ=255)                                                   cTGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcctg              <  1:241875/71‑1 (MQ=255)                                                    tGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcctgc             >  1:337012/1‑71 (MQ=255)                                                    tGTATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcctgc             >  1:2386727/1‑71 (MQ=255)                                                      tagcGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcctgc             >  1:1307416/4‑69 (MQ=255)                                                      tATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcctgc             >  1:1221014/1‑69 (MQ=255)                                                      tATCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcc                >  1:584820/1‑66 (MQ=255)                                                        tCGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCctgcctgc         <  1:1775771/71‑1 (MQ=255)                                                         cGCAACAATCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTcct               <  1:813403/64‑1 (MQ=255)                                                           caacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAg      >  1:2967032/1‑71 (MQ=255)                                                           caacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAg      >  1:1049808/1‑71 (MQ=255)                                                            aacaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAgg     >  1:2569802/1‑71 (MQ=255)                                                             acaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGAc                          >  1:2709784/1‑49 (MQ=255)                                                             acaaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAggg    <  1:2285938/71‑1 (MQ=255)                                                              caaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGc   <  1:2045342/71‑1 (MQ=255)                                                              caaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGc   >  1:1245479/1‑71 (MQ=255)                                                               aaTCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCt  >  1:884524/1‑71 (MQ=255)                                                                 tCCGTGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGgcgc                     <  1:1086179/50‑1 (MQ=255)                                                                     |                                                                 AATGGTTAAGTGGTGACAAACCCTCGAAAGAGGTGCTCTTTCATCTGGCGCTGTATCGCAACAATCCGCGCTGTAAAGCGGTGGTGCATTTGCACAGCACATGGTCGACGGCGCTTTCCTGCCTGCAAGGGCT  >  minE/1833463‑1833595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑