| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 1,867,615 | G→A | T429I (ACC→ATC) | ygcU ← | predicted FAD containing dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,867,615 | 0 | G | A | 98.9% | 267.7 / ‑4.1 | 88 | T429I (ACC→ATC) | ygcU | predicted FAD containing dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (41/46); total (41/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
GTTTGCTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTA > minE/1867547‑1867677 | gTTTGCTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGAtt > 1:2885378/1‑71 (MQ=255) tttGCTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGAtt > 1:2410904/1‑70 (MQ=255) ttGCTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttc > 1:1030593/1‑71 (MQ=255) ttGCTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttc > 1:161670/1‑71 (MQ=255) tGCTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttc < 1:499784/70‑1 (MQ=255) gCTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttct < 1:1480263/70‑1 (MQ=255) cTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttcttc < 1:1747161/71‑1 (MQ=255) cTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttctt < 1:1344324/70‑1 (MQ=255) cTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttctt < 1:1333709/70‑1 (MQ=255) ccAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATttc > 1:1628306/1‑66 (MQ=255) cAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTcaca < 1:960063/71‑1 (MQ=255) cAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTca > 1:2027525/1‑69 (MQ=255) cAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTca < 1:2891474/69‑1 (MQ=255) cAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTca > 1:854797/1‑69 (MQ=255) cAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTca > 1:843887/1‑69 (MQ=255) cAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTca > 1:2470520/1‑69 (MQ=255) gTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACAGCCGAGGCGAATGATTTCTTca > 1:271666/1‑67 (MQ=255) tGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgat < 1:1394784/71‑1 (MQ=255) aaCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatga < 1:1624226/71‑1 (MQ=255) aCGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatgat > 1:3047559/1‑71 (MQ=255) gcgATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg > 1:2437252/1‑67 (MQ=255) aTGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCttgt > 1:1670628/1‑71 (MQ=255) tGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAga > 1:244135/1‑61 (MQ=255) gTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCttgtt < 1:721122/70‑1 (MQ=255) tttACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCttgttg < 1:1086561/70‑1 (MQ=255) tttACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGa < 1:1579292/71‑1 (MQ=255) tttACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACACATGATCTTGTTGa < 1:327407/71‑1 (MQ=255) tttACCGATACCGTGGTGGTGCACCATCGAACCACCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGa < 1:2072917/71‑1 (MQ=255) ttACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCttgttg < 1:2898252/69‑1 (MQ=255) ttACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAg > 1:192209/1‑71 (MQ=255) tACCGATACCGTGGTGGTGCACCATCGAACCGCCGATGCGAATGATTTCTTCACAGATGATCttgtt > 1:1175606/1‑67 (MQ=255) tACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCttgtt > 1:2552469/1‑67 (MQ=255) tACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAg < 1:2120143/70‑1 (MQ=255) tACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAg < 1:2582905/70‑1 (MQ=255) tACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTAACAGATGATCttgttg < 1:2213430/68‑1 (MQ=255) aTACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGAtt > 1:1520887/1‑71 (MQ=255) aTACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGAtt < 1:1824527/71‑1 (MQ=255) aCCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtgt > 1:1803627/1‑71 (MQ=255) aCCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtgt > 1:2565530/1‑71 (MQ=255) ccGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtgtg > 1:512879/1‑71 (MQ=255) tggtggtgCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGa > 1:2312521/1‑58 (MQ=255) gtggtgCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTAc < 1:4879/70‑1 (MQ=255) tgCACCATCGAACCGCCGAGGCGAATGATTACTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTc > 1:2587855/1‑71 (MQ=255) gCACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtg < 1:2762438/59‑1 (MQ=255) cACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCg < 1:493761/70‑1 (MQ=255) cACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCg < 1:908010/70‑1 (MQ=255) cACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCg < 1:1520673/70‑1 (MQ=255) cACCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGa < 1:362584/71‑1 (MQ=255) aCCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATtgtg > 1:2297949/1‑59 (MQ=255) aCCATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAt < 1:2957830/71‑1 (MQ=255) ccATCGAACCGCCGAGGCGAATGATTTCTTCACAGATTATCTTGTTGAGCGGa < 1:2795076/53‑1 (MQ=255) ccATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGa < 1:1439406/53‑1 (MQ=255) cATCGAACCGCCGAGGCGAATGATTTCTTCACAgatg > 1:2001174/1‑37 (MQ=255) cATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCt < 1:2141096/41‑1 (MQ=255) cATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCgg < 1:2235257/51‑1 (MQ=255) cATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTAc < 1:2165901/61‑1 (MQ=255) cATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAttt > 1:2882396/1‑71 (MQ=255) cATCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAttt > 1:1387738/1‑71 (MQ=255) tCGAACCGCCGAGGCGAATGATTTCTTCACAgatg < 1:2767814/35‑1 (MQ=255) tCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCttgttg < 1:1358825/44‑1 (MQ=255) tCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGAt > 1:2558152/1‑51 (MQ=255) tCGAACCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTcc < 1:76840/71‑1 (MQ=255) gAACTGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCctc < 1:625346/71‑1 (MQ=255) gAACCGCCGAGGCGAATGATTTCTTCACAGATGATCttgt > 1:2211538/1‑40 (MQ=255) aaCCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGAt > 1:221418/1‑48 (MQ=255) aaCCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGAt > 1:744029/1‑48 (MQ=255) aaCCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCctct > 1:1545209/1‑71 (MQ=255) aCCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCtt < 1:926603/71‑1 (MQ=255) aCCGCCGAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCtt < 1:2563037/71‑1 (MQ=255) ccgccgAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTc > 1:1361568/1‑71 (MQ=255) ctccgAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTg < 1:2557250/54‑1 (MQ=255) gccgAGTCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCg > 1:393318/1‑71 (MQ=255) gccgAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTTGATTTCCTCTTCCg < 1:1465633/71‑1 (MQ=255) ccgAGGCGAATGATTTCTTCACAGATTATCTTGTTGAGCGGATTGTGGTa < 1:652634/50‑1 (MQ=255) ccgAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCgg > 1:547498/1‑71 (MQ=255) ccgAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCgg > 1:2716134/1‑71 (MQ=255) cgAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCgg < 1:1996333/70‑1 (MQ=255) cgAGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGc > 1:2994882/1‑71 (MQ=255) gAGGCGAATGATTTCTTCACAGATGATCTCGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCt < 1:2025295/71‑1 (MQ=255) aGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGa < 1:1574831/39‑1 (MQ=255) aGGCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCt > 1:2095834/1‑70 (MQ=255) gCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGAtt > 1:1217671/1‑39 (MQ=255) gCGAATGATTTCTTCACAGATGATCTTGTTGAGCGGAtt > 1:2890581/1‑39 (MQ=255) cGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTa < 1:1417979/44‑1 (MQ=255) cGAATGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTa > 1:2889499/1‑69 (MQ=255) aaTGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGAt < 1:772494/51‑1 (MQ=255) aTGATTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCtt < 1:1696104/65‑1 (MQ=255) tGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGa < 1:977322/48‑1 (MQ=255) | GTTTGCTCCAGTGAACGCGATGTTTACCGATACCGTGGTGGTGCACCATCGAACCGCCGAGGCGAATGGTTTCTTCACAGATGATCTTGTTGAGCGGATTGTGGTACTTGTCGATTTCCTCTTCCGGCTTA > minE/1867547‑1867677 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |