Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,029,965	A→G	F294L (TTC→CTC)	parE ←	DNA topoisomerase IV, subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,029,965	0	A	G	100.0%	240.2 / NA	67	F294L (TTC→CTC)	parE	DNA topoisomerase IV, subunit B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (43/24);  total (43/24)

GCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGG  >  minE/2029897‑2030031                                                                     |                                                                   gcAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCt                                                                  >  1:1257717/1‑71 (MQ=255)  cAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCt                                                                  <  1:2798330/70‑1 (MQ=255)        tCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCAt                                                           <  1:715355/71‑1 (MQ=255)        tCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCAt                                                           >  1:2985730/1‑71 (MQ=255)        tCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCAt                                                           >  1:774577/1‑71 (MQ=255)         cccAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATc                                                          >  1:1411372/1‑71 (MQ=255)          ccATATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCt                                                                  <  1:245141/62‑1 (MQ=255)          ccAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATc                                                          <  1:456/70‑1 (MQ=255)            aGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATcgcg                                                       >  1:1831079/1‑71 (MQ=255)            aGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATcgcg                                                       >  1:6352/1‑71 (MQ=255)              atatCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTc                                                     >  1:1326186/1‑71 (MQ=255)              atatCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTc                                                     >  1:3050064/1‑71 (MQ=255)                 tCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCaa                                                  >  1:2453223/1‑71 (MQ=255)                     ccgccgACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAAc                                                 <  1:1674876/68‑1 (MQ=255)                     ccgccgACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACa                                                <  1:2954413/69‑1 (MQ=255)                     ccgccgACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAgg                                              >  1:2593041/1‑71 (MQ=255)                       gccgACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGcc                                            >  1:2154948/1‑71 (MQ=255)                          gACAGATTTACACCGCGCGGCAGAGTATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTg                                         <  1:1643594/71‑1 (MQ=255)                           aCAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCa                                                                >  1:2585920/1‑47 (MQ=255)                           aCAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCa                                                                >  1:758462/1‑47 (MQ=255)                           aCAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCa                                                                >  1:640443/1‑47 (MQ=255)                            cAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGa                                        <  1:2553557/70‑1 (MQ=255)                            cAGCTTTACACAGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGAc                                       >  1:2386236/1‑71 (MQ=255)                             aGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACg                                      >  1:442011/1‑71 (MQ=255)                               cTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACg                                      >  1:2809091/1‑69 (MQ=255)                               cTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACg                                      >  1:1130606/1‑69 (MQ=255)                               cTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCa                                    <  1:1860354/71‑1 (MQ=255)                                 ttACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCCTCGCGTCCAAc                                                 >  1:2388037/1‑56 (MQ=255)                                  tACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGc                                                             >  1:1359874/1‑43 (MQ=255)                                  tACACCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTTACGCAg                                   <  1:392296/69‑1 (MQ=255)                                   acacCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGc                                             >  1:2263403/1‑58 (MQ=255)                                     acCGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCAt                              <  1:643579/71‑1 (MQ=255)                                      ccGCGCGGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCAt                              >  1:280407/1‑70 (MQ=255)                                       cgcgcgGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCAtt                             <  1:488804/70‑1 (MQ=255)                                        gcgcgGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTaa                           >  1:2339294/1‑71 (MQ=255)                                        gcgcgGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTa                            >  1:1371982/1‑70 (MQ=255)                                         cgcgGCAGAATATTGCGGTATTCACAGAGCTCACGCATTGCGTCCAACAGGCCCTGACGCAGACCa                               >  1:2167151/1‑66 (MQ=255)                                         cgcgGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTaa                           >  1:2740013/1‑70 (MQ=255)                                         cgcgGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTa                            >  1:2909853/1‑69 (MQ=255)                                          gcgGCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTcc                                                    <  1:130774/44‑1 (MQ=255)                                             gCAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATgg                      <  1:2425017/71‑1 (MQ=255)                                              cAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATggg                     <  1:144741/71‑1 (MQ=255)                                              cAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATggg                     >  1:2475971/1‑71 (MQ=255)                                              cAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATggg                     >  1:838781/1‑71 (MQ=255)                                              cAGAATATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATggg                     >  1:1490108/1‑71 (MQ=255)                                                 aaTATTGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTAc                  >  1:1190868/1‑71 (MQ=255)                                                   tatTGCGGTATTCACAGAGCTCACGCATCGCGTCCa                                                   >  1:1755699/1‑36 (MQ=255)                                                   tatTGCGGTATTCACAGAGCTCACGCATCGCGTCCa                                                   >  1:636375/1‑36 (MQ=255)                                                     ttGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACa                         >  1:1549778/1‑60 (MQ=255)                                                     ttGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACa                         >  1:1965014/1‑60 (MQ=255)                                                     ttGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGcc              <  1:889815/71‑1 (MQ=255)                                                      tGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCAtt                             <  1:2894690/55‑1 (MQ=255)                                                      tGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGcc              >  1:604073/1‑70 (MQ=255)                                                      tGCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGcc              >  1:226500/1‑70 (MQ=255)                                                       gCGGTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGa                                        >  1:1114297/1‑43 (MQ=255)                                                         ggTATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGcc              >  1:404108/1‑67 (MQ=255)                                                           tATTCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCa                                    <  1:890599/43‑1 (MQ=255)                                                             ttCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCg      >  1:2343320/1‑71 (MQ=255)                                                             ttCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCg      >  1:2278454/1‑71 (MQ=255)                                                             ttCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCg      <  1:1893464/71‑1 (MQ=255)                                                             ttCACAGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCg      >  1:1493297/1‑71 (MQ=255)                                                               cacaGAGCTCACGCATCGCGTCCAACAGGCCCTGa                                        >  1:787580/1‑35 (MQ=255)                                                                acaGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGtt    <  1:2961119/70‑1 (MQ=255)                                                                acaGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGtt    <  1:1642032/70‑1 (MQ=255)                                                                 caGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCAtt                             <  1:2956428/44‑1 (MQ=255)                                                                 caGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTgg  <  1:2498767/71‑1 (MQ=255)                                                                 caGAGCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTgg  <  1:108301/71‑1 (MQ=255)                                                                  agagCTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTgg  >  1:64594/1‑70 (MQ=255)                                                                     |                                                                   GCAGCGATCCCAGATATCTTCCGCCGACAGCTTTACACCGCGCGGCAGAATATTGCGGTATTCACAGAACTCACGCATCGCGTCCAACAGGCCCTGACGCAGACCATTAACATGGGTACCGCCCTGCATCGTTGG  >  minE/2029897‑2030031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑