Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,364,103	A→G	W456R (TGG→CGG)	trkH ←	potassium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,364,103	0	A	G	100.0%	229.6 / NA	64	W456R (TGG→CGG)	trkH	potassium transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/35);  total (29/35)

GTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAGGTTAT  >  minE/2364039‑2364168                                                                 |                                                                  gTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                             >  1:363429/1‑71 (MQ=255)    aaGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCAc                                                          >  1:1907670/1‑71 (MQ=255)    aaGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCAc                                                          >  1:3026853/1‑71 (MQ=255)        gCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCgg                                                       >  1:65271/1‑70 (MQ=255)         cACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGt                                                     <  1:1802840/71‑1 (MQ=255)           ccAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCggg                                                      >  1:2839930/1‑68 (MQ=255)            cAGCATTGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCTGGATCCGTTTAGCCACCGGGTTCa                                                  <  1:444492/71‑1 (MQ=255)            cAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCa                                                  <  1:1365987/71‑1 (MQ=255)              gCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTa                                                               >  1:693163/1‑56 (MQ=255)               cAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATAc                                               >  1:1081831/1‑71 (MQ=255)               cAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATAc                                               >  1:1929473/1‑71 (MQ=255)                   gTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTgg                                            >  1:1904041/1‑70 (MQ=255)                    tAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGTATCCGTTTAGCCACCGGGTTCATACTGGTa                                          <  1:2660718/71‑1 (MQ=255)                    tAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGc                                                             >  1:2478966/1‑52 (MQ=255)                    tAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGt                                           >  1:89762/1‑70 (MQ=255)                     aaaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                          <  1:1909740/70‑1 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGtt                                                    <  1:922689/59‑1 (MQ=255)                      aaGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGtt                                                    <  1:1231318/59‑1 (MQ=255)                       aGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATAc                                               <  1:1361812/63‑1 (MQ=255)                       aGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATAc                                               <  1:1819585/63‑1 (MQ=255)                       aGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAg                                       <  1:1100819/71‑1 (MQ=255)                         aCCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGtt                                     <  1:625955/71‑1 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                                    <  1:2435750/71‑1 (MQ=255)                          ccTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                                    >  1:2632621/1‑71 (MQ=255)                          ccTCGAGACGACAAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                                    >  1:74314/1‑71 (MQ=255)                           cTCGAGACTACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTAt                                   <  1:1082955/71‑1 (MQ=255)                           cTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCgg                                                       <  1:1683739/51‑1 (MQ=255)                           cTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTa                                    <  1:961510/70‑1 (MQ=255)                           cTCGAGACGACCAAACAGCATGATGGCAATCAGGATCCGTTTAGCCACCgg                                                       <  1:365753/51‑1 (MQ=39)                           cTCGAGAAGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTAt                                   >  1:208008/1‑71 (MQ=255)                            tCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTgg                                            <  1:770110/61‑1 (MQ=255)                            tCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTAt                                   <  1:687994/70‑1 (MQ=255)                             cGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGtt                                                                 >  1:489950/1‑39 (MQ=255)                             cGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTgg                                            >  1:306522/1‑60 (MQ=255)                             cGAGACGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTAt                                   >  1:2133223/1‑69 (MQ=255)                              gagaCGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGt                                           >  1:958647/1‑60 (MQ=255)                              gagaCGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTAt                                   >  1:2588321/1‑68 (MQ=255)                               agaCGAGCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAg                                >  1:393877/1‑70 (MQ=255)                               agaCGACCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGc                               <  1:1329107/71‑1 (MQ=255)                                 acgacCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCgg                                                       <  1:1209594/45‑1 (MQ=255)                                  cgacCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAATTATCAGCAAc                            <  1:2299089/71‑1 (MQ=255)                                   gacCAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCa                                                  <  1:2422837/48‑1 (MQ=255)                                      cAAACAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGtt                                     <  1:2914412/58‑1 (MQ=255)                                        aaCAGCATGTTGGCAATCAGGATCCGTTTAGCCAc                                                          >  1:1552860/1‑35 (MQ=255)                                        aaCAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCa                                                  <  1:2131037/43‑1 (MQ=255)                                        aaCAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCa                                                  <  1:127214/43‑1 (MQ=255)                                        aaCAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTaaa                                        >  1:1273487/1‑53 (MQ=255)                                        aaCAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGc                       >  1:1412182/1‑70 (MQ=255)                                         aCAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCa                                 >  1:2595376/1‑59 (MQ=255)                                         aCAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCa                     <  1:857116/71‑1 (MQ=255)                                          cAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCaa                    <  1:2087146/71‑1 (MQ=255)                                          cAGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCaa                    <  1:1837527/71‑1 (MQ=255)                                           aGCATGTTGGCAATCAGGATCCGTTTAGCCACCgg                                                       >  1:1694438/1‑35 (MQ=255)                                           aGCATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCAAACTGGTAAAGTTATCAGCAACCACGCCAAg                   >  1:473996/1‑71 (MQ=255)                                            gCATGTTGGCTTTCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGtt                                     <  1:904986/52‑1 (MQ=255)                                             cATGTTGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGcc                 <  1:483827/71‑1 (MQ=255)                                                 ttGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTa                                          <  1:1261787/42‑1 (MQ=255)                                                 ttGGCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTgg             >  1:1861097/1‑71 (MQ=255)                                                    gCAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGcc                      >  1:1294361/1‑59 (MQ=255)                                                     cAATCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGcccc         <  1:255197/71‑1 (MQ=255)                                                       aTCAGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCa                              <  1:2083583/48‑1 (MQ=255)                                                          aGGATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAGGt     <  1:1664755/70‑1 (MQ=255)                                                            gATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAGGTTAt  <  1:1310642/71‑1 (MQ=255)                                                            gATCCGTTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAGGTTAt  >  1:447222/1‑71 (MQ=255)                                                                 |                                                                  GTAAAGAGCACCAGCAATGTAAAGACCTCGAGACGACCAAACAGCATGTTGGCAATCAGGATCCATTTAGCCACCGGGTTCATACTGGTAAAGTTATCAGCAACCACGCCAAGCCCTGGCCCCAGGTTAT  >  minE/2364039‑2364168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑