BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,371,447	A→G	F214L (TTT→CTT)	fre ←	flavin reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,371,447	0	A	G	100.0%	207.6 / NA	59	F214L (TTT→CTT)	fre	flavin reductase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (29/30); total (29/30)

ATATCTCAGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGCCAGCG  >  minE/2371380‑2371512

atatCTCAGATAAATGCAAACGAATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCa                                                                 <  1:251106/70‑1 (MQ=255)

 tatCTCAGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCa                                                                 <  1:616555/69‑1 (MQ=255)

      cAGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATcgcg                                                          >  1:218045/1‑71 (MQ=255)

      cAGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATcgcg                                                          >  1:1521182/1‑71 (MQ=255)

       aGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATc                                                             <  1:2868006/67‑1 (MQ=255)

           aaaTGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCaa                                                     >  1:776105/1‑71 (MQ=255)

           aaaTGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCaa                                                     >  1:517979/1‑71 (MQ=255)

            aaTGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAAt                                                    >  1:243334/1‑71 (MQ=255)

              tGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAAttt                                                  <  1:419136/71‑1 (MQ=255)

                cAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTc                                                >  1:1272406/1‑71 (MQ=255)

                  aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                              >  1:1037942/1‑71 (MQ=255)

                  aaCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGc                                              >  1:2390283/1‑71 (MQ=255)

                   aCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCgg                                                         >  1:1755325/1‑59 (MQ=255)

                   aCGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGcc                                             <  1:1575175/71‑1 (MQ=255)

                    cGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCa                                            >  1:857289/1‑71 (MQ=255)

                      cATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAtc                                          <  1:2125036/71‑1 (MQ=255)

                       aTCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCa                                                                 <  1:955192/47‑1 (MQ=255)

                       aTCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCa                                                                 <  1:2060566/47‑1 (MQ=255)

                       aTCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAtc                                          <  1:1871981/70‑1 (MQ=255)

                        tCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCACGGGCAATTTTCGCCAtctc                                        <  1:1332401/71‑1 (MQ=255)

                         cGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCAtctc                                        >  1:2048995/1‑70 (MQ=255)

                          gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCaa                                      <  1:523770/71‑1 (MQ=255)

                          gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCa                                       <  1:581583/70‑1 (MQ=255)

                          gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCa                                       <  1:2129246/70‑1 (MQ=255)

                          gCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCa                                       <  1:2653069/70‑1 (MQ=255)

                           ccAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCAATCTCa                                       <  1:205427/69‑1 (MQ=255)

                             aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATcgc                                                           >  1:1533652/1‑47 (MQ=255)

                             aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATcgc                                                           >  1:251330/1‑47 (MQ=255)

                             aaaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:2630211/71‑1 (MQ=255)

                              aaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACg                                  <  1:2479113/71‑1 (MQ=255)

                              aaCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACg                                  >  1:1954193/1‑71 (MQ=255)

                               aCAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGCGCAATTTTCGCCATCTCAAAACGt                                 <  1:54559/71‑1 (MQ=255)

                                cAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATcg                                                            >  1:635110/1‑43 (MQ=255)

                                 aGGCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCaa                                      <  1:2991637/64‑1 (MQ=255)

                                   gCGATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCaa                                      <  1:3003778/62‑1 (MQ=255)

                                     gATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATcgc                                                           <  1:2481480/39‑1 (MQ=255)

                                     gATCTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGc                            >  1:871564/1‑70 (MQ=255)

                                        cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                        >  1:2874029/1‑71 (MQ=255)

                                        cTTCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                        >  1:759503/1‑71 (MQ=255)

                                         ttCCCGCGCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                        >  1:2293040/1‑70 (MQ=255)

                                             cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAAc                                   <  1:299671/55‑1 (MQ=255)

                                             cgcgCATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGa                    >  1:2561235/1‑70 (MQ=255)

                                                 cATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCa               <  1:379811/71‑1 (MQ=255)

                                                 cATTACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCa               <  1:2657548/71‑1 (MQ=255)

                                                   ttACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCAt              >  1:1816691/1‑70 (MQ=255)

                                                   ttACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATg             >  1:1813337/1‑71 (MQ=255)

                                                    tACGCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATg             >  1:2375667/1‑70 (MQ=255)

                                                       gCTCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAata                        <  1:2901631/56‑1 (MQ=255)

                                                         tCACTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGc       >  1:886514/1‑71 (MQ=255)

                                                           aCTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGCCa     <  1:2736087/71‑1 (MQ=255)

                                                            cTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCaa                                      <  1:2075387/37‑1 (MQ=255)

                                                            cTGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGat                   <  1:1528448/56‑1 (MQ=255)

                                                             tGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCAt              >  1:1591530/1‑60 (MQ=255)

                                                             tGCAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCAt              >  1:124491/1‑60 (MQ=255)

                                                               cAAAGCAGATCGCTGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGa                    >  1:555575/1‑52 (MQ=255)

                                                               cAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGat                   >  1:1564786/1‑53 (MQ=255)

                                                               cAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGa                    <  1:288839/52‑1 (MQ=255)

                                                               cAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGCCAGCg  <  1:2868651/70‑1 (MQ=255)

                                                               cAAAGCAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGCCAGCg  >  1:1786093/1‑70 (MQ=255)

ATATCTCAGATAAATGCAAACGCATCGCCAAACAGGCGATCTTCCCGCGCATTACGCTCACTGCAAAACAGATCGCGGGCAATTTTCGCCATCTCAAAACGTCCGGCAATATAGATATCATGCTCTGCCAGCG  >  minE/2371380‑2371512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`