Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,473,718	C→T	F331F (TTC→TTT)	glmS →	L‑glutamine:D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,473,718	0	C	T	100.0%	227.8 / NA	74	F331F (TTC→TTT)	glmS	L‑glutamine:D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/30);  total (44/30)

TTTCCCGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAA  >  minE/2473654‑2473782                                                                 |                                                                 tttCCCGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTAt                                                            >  1:91004/1‑71 (MQ=255)   tCCCGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCg                                                          >  1:1626436/1‑71 (MQ=255)      cGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGc                                                         >  1:1148754/1‑69 (MQ=255)      cGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCa                                                        >  1:1144928/1‑70 (MQ=255)      cGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCa                                                        >  1:1340258/1‑70 (MQ=255)        cTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCg                                                                >  1:2215966/1‑60 (MQ=255)        cTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCaaa                                                      >  1:519500/1‑70 (MQ=255)        cTACTCGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAAt                                                     >  1:2680365/1‑71 (MQ=255)           cTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATc                                                    <  1:2012182/69‑1 (MQ=255)             ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc                                                >  1:2931970/1‑71 (MQ=255)             ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc                                                >  1:156965/1‑71 (MQ=255)             ggTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGc                                                 >  1:1719245/1‑70 (MQ=255)              gTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGcc                                                <  1:688041/70‑1 (MQ=255)                ttGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTAt                                                            >  1:2827296/1‑56 (MQ=255)                 tGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTg                                             <  1:1938432/70‑1 (MQ=255)                   aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGc                                                 >  1:2365164/1‑64 (MQ=255)                   aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcg                                           <  1:2293861/70‑1 (MQ=255)                   aaTCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcg                                           <  1:2420241/70‑1 (MQ=255)                     tCGCTAGCAGGTATTCCGTGCGACGTCGAATACGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgtcg                                        >  1:2131278/1‑71 (MQ=255)                     tCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTg                                                  >  1:634769/1‑61 (MQ=255)                       gCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgtcgt                                       >  1:1902921/1‑70 (MQ=255)                        cTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGt                                              <  1:3024397/62‑1 (MQ=255)                           gCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACa                                   <  1:888506/70‑1 (MQ=255)                           gCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAg                                  <  1:1106902/71‑1 (MQ=255)                             aGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGcc                                <  1:2143603/71‑1 (MQ=255)                                 aTTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgtcgt                                       <  1:755619/60‑1 (MQ=255)                                 aTTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGcgtcgt                                       <  1:195207/60‑1 (MQ=255)                                 aTTCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCt                               >  1:1448828/1‑68 (MQ=255)                                 aTTCCGTGCGACGTCGAAATCGCCTATGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtga                             >  1:2788273/1‑70 (MQ=255)                                   tCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTa                                                             <  1:2990526/36‑1 (MQ=255)                                   tCCGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCt                               <  1:1959113/66‑1 (MQ=255)                                    ccGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAAt                                                     >  1:1586393/1‑43 (MQ=255)                                    ccGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAAt                                                     >  1:2276950/1‑43 (MQ=255)                                    ccGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAAt                                                     >  1:1184258/1‑43 (MQ=255)                                    ccGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTgatgat                         >  1:1776572/1‑71 (MQ=255)                                    ccGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTgatgat                         >  1:1614038/1‑71 (MQ=255)                                    ccGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTgatgat                         >  1:1465237/1‑71 (MQ=255)                                    ccGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTgatgat                         >  1:2569982/1‑71 (MQ=255)                                     cGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTgatgat                         >  1:481290/1‑70 (MQ=255)                                     cGTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCAGTGCGTCGTAACAGCCTGATGATc                        <  1:2964297/71‑1 (MQ=255)                                      gTGCGACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCt                               >  1:437278/1‑63 (MQ=255)                                          gACGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTaa                                     <  1:2301322/53‑1 (MQ=255)                                           aCGTCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTa                                      >  1:1305038/1‑51 (MQ=255)                                              tCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTca               <  1:1021922/71‑1 (MQ=255)                                              tCGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTca               <  1:139362/71‑1 (MQ=255)                                               cGAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCt                               <  1:1782402/54‑1 (MQ=255)                                               cGAAATCGCATCTGAATTTCGCTATAGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTca               >  1:1292624/1‑70 (MQ=255)                                                gAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTgatgat                         <  1:961257/59‑1 (MQ=255)                                                gAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTgatgat                         <  1:2998987/59‑1 (MQ=255)                                                gAAATCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTca               >  1:582142/1‑69 (MQ=255)                                                  aaTCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTcaca             >  1:2610580/1‑69 (MQ=255)                                                  aaTCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGt           >  1:1258723/1‑71 (MQ=255)                                                  aaTCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGt           >  1:2294629/1‑71 (MQ=255)                                                  aaTCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGt           >  1:1562063/1‑71 (MQ=255)                                                   aTCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtgatga                          <  1:2600118/55‑1 (MQ=255)                                                    tCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTgatgat                         >  1:970801/1‑55 (MQ=255)                                                    tCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCt         <  1:1306374/71‑1 (MQ=255)                                                    tCGCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCt         <  1:2474224/71‑1 (MQ=255)                                                      gCCTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCT‑‑TCACAGTCTgg       >  1:2774136/1‑69 (MQ=255)                                                       ccTCTGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGc      >  1:2057177/1‑71 (MQ=255)                                                         tctGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTc          <  1:44343/65‑1 (MQ=255)                                                         tctGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCg     >  1:1044115/1‑70 (MQ=255)                                                         tctGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCg     >  1:1684884/1‑70 (MQ=255)                                                          ctGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCtgatga                          <  1:642322/48‑1 (MQ=255)                                                          ctGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGa    >  1:298761/1‑70 (MQ=255)                                                          ctGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGa    >  1:878398/1‑70 (MQ=255)                                                           tGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTg        <  1:1452753/65‑1 (MQ=255)                                                           tGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGaaa  >  1:1555901/1‑71 (MQ=255)                                                           tGAATTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGa    <  1:266791/69‑1 (MQ=255)                                                             aaTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTg                  >  1:2995790/1‑53 (MQ=255)                                                             aaTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGaa   <  1:1788666/68‑1 (MQ=255)                                                             aaTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGaa   <  1:1195199/68‑1 (MQ=255)                                                             aaTTTCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGaa   <  1:2964654/68‑1 (MQ=255)                                                              aTTTCGATATAGCAAATCTGCCGTGCGTCGTAACAGCCt                               >  1:270239/1‑39 (MQ=37)                                                                 |                                                                 TTTCCCGCTACTGGTTTGAATCGCTAGCAGGTATTCCGTGCGACGTCGAAATCGCCTCTGAATTCCGCTATCGCAAATCTGCCGTGCGTCGTAACAGCCTGATGATCACCTTGTCACAGTCTGGCGAAA  >  minE/2473654‑2473782

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑