Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,516,656	C→T	A102T (GCT→ACT)	trmH ←	tRNA (Guanosine‑2'‑O‑)‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,516,656	0	C	T	100.0%	292.7 / NA	98	A102T (GCT→ACT)	trmH	tRNA (Guanosine‑2'‑O‑)‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (48/50);  total (48/50)

CCCGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGCC  >  minE/2516589‑2516722                                                                    |                                                                   cccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGtt                                                                 <  1:1879730/71‑1 (MQ=255)  ccGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                                <  1:678185/71‑1 (MQ=255)    gTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTAt                                                               >  1:1022701/1‑70 (MQ=255)     ttttCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTAt                                                               >  1:1391794/1‑69 (MQ=255)       ttCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAg                                                            >  1:2250293/1‑70 (MQ=255)       ttCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAg                                                            >  1:1237140/1‑70 (MQ=255)         ctcCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGa                                                           >  1:376091/1‑69 (MQ=255)            cTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGaaa                                                         <  1:2332451/68‑1 (MQ=255)              gTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATgg                                                    >  1:2705856/1‑71 (MQ=255)               tCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGa                                                           <  1:955956/63‑1 (MQ=255)               tCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATggg                                                   <  1:480157/71‑1 (MQ=255)               tCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATggg                                                   >  1:1656012/1‑71 (MQ=255)               tCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATAAGAAAGATggg                                                   <  1:202188/71‑1 (MQ=255)               tCCCATCAAAATGCAGGTCGG‑‑GAGTGTAATCAATTTCGCTGAAATCGACAGTGTTATCAGAAAGATggg                                                   <  1:1961618/69‑1 (MQ=255)                cccATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGt                                                  >  1:1642344/1‑71 (MQ=255)                 ccATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGt                                                  <  1:2700333/70‑1 (MQ=255)                 ccATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGt                                                  <  1:2863369/70‑1 (MQ=255)                   aTCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc                                               <  1:1700189/71‑1 (MQ=255)                    tCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGa                                                           <  1:2201360/58‑1 (MQ=255)                    tCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc                                               >  1:538536/1‑70 (MQ=255)                    tCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc                                               >  1:2998645/1‑70 (MQ=255)                     cAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc                                               >  1:111516/1‑69 (MQ=255)                      aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                                >  1:2783842/1‑51 (MQ=255)                      aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTa                                                                >  1:207352/1‑51 (MQ=255)                      aaaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc                                               >  1:1817343/1‑68 (MQ=255)                      aaaaGGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc                                               >  1:2323026/1‑68 (MQ=255)                       aaaTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTAt                                                               <  1:780241/51‑1 (MQ=255)                        aaTGCAGGTCGGTCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGaa                                          <  1:1528611/71‑1 (MQ=255)                         aTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAAt                                         <  1:1657412/71‑1 (MQ=255)                         aTGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAAt                                         <  1:2137617/71‑1 (MQ=255)                           gCAGGTCGGGCGAGTGTAATCAATTTCTCGGAAATCGACAGTGTTAt                                                               <  1:2930330/47‑1 (MQ=255)                            cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCt                                       <  1:2286286/70‑1 (MQ=255)                            cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg                                      >  1:1021180/1‑71 (MQ=255)                            cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg                                      >  1:136631/1‑71 (MQ=255)                            cAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg                                      <  1:2958931/71‑1 (MQ=255)                               gTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGtt                                                                 >  1:1371266/1‑41 (MQ=255)                               gTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGtt                                                                 >  1:1945910/1‑41 (MQ=255)                               gTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGaaa                                                         <  1:2917274/49‑1 (MQ=255)                               gTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATg                                                     <  1:1667068/53‑1 (MQ=255)                               gTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGc                                     <  1:1494616/69‑1 (MQ=255)                               gTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCAt                                   >  1:254666/1‑71 (MQ=255)                                tCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATgg                                                    >  1:562229/1‑53 (MQ=255)                                 cGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAg                                                        >  1:2214802/1‑48 (MQ=255)                                  gggCGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGc                                 <  1:1833152/70‑1 (MQ=255)                                     cGAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGaaa                                                         <  1:2095566/43‑1 (MQ=255)                                      gAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTgg                            >  1:94868/1‑71 (MQ=255)                                      gAGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTgg                            <  1:848804/71‑1 (MQ=255)                                       aGTGTAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCa                                    >  1:1348909/1‑62 (MQ=255)                                        gtgtAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGc                                               >  1:2234984/1‑50 (MQ=255)                                         tgtAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCt                         <  1:111473/71‑1 (MQ=255)                                           tAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGcc                                >  1:862147/1‑62 (MQ=255)                                           tAATCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt                       >  1:1776405/1‑71 (MQ=255)                                           tAATCAATTTCGAGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGcc                                >  1:85888/1‑62 (MQ=255)                                            aaTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGa                                           <  1:47183/50‑1 (MQ=255)                                            aaTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt                       >  1:97922/1‑70 (MQ=255)                                            aaTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt                       >  1:2564506/1‑70 (MQ=255)                                             aTCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTg                      <  1:1904333/70‑1 (MQ=255)                                             aTCAATTTCGCGGAAATCGACAGTGGTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTg                      <  1:45384/70‑1 (MQ=255)                                              tCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg                                      <  1:717202/53‑1 (MQ=255)                                              tCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTgg                            >  1:2195875/1‑63 (MQ=255)                                              tCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTgg                            >  1:2741098/1‑63 (MQ=255)                                              tCAATTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTgag                    <  1:1509351/71‑1 (MQ=255)                                                aaTTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGc                           >  1:1556230/1‑62 (MQ=255)                                                 aTTTCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCt                         <  1:1711577/63‑1 (MQ=255)                                                  tttCGCGGAAATCGACAGTGTTATCAGAAAGATggg                                                   <  1:208960/36‑1 (MQ=255)                                                  tttCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCttt                       <  1:434564/64‑1 (MQ=255)                                                  tttCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGAt                  >  1:1667106/1‑69 (MQ=255)                                                  tttCGCGGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGAt                  >  1:1670578/1‑69 (MQ=255)                                                       cgGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGTCCTTTGAGATGAGCGAc           >  1:146207/1‑71 (MQ=255)                                                       cgGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGAc           <  1:2972842/71‑1 (MQ=255)                                                       cgGAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGAc           <  1:1294728/71‑1 (MQ=255)                                                        ggAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTg                      <  1:1908181/59‑1 (MQ=255)                                                        ggAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATg                 <  1:715903/64‑1 (MQ=255)                                                         gAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTg                             <  1:2086252/51‑1 (MQ=255)                                                         gAAATCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTga                     <  1:1226416/59‑1 (MQ=255)                                                          aaaTCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg                                      <  1:2652752/41‑1 (MQ=255)                                                          aaaTCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACgg         >  1:922189/1‑70 (MQ=255)                                                          aaaTCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACgg         >  1:1557295/1‑70 (MQ=255)                                                           aaTCGAGAGTGTTATCAGAAAGATGGGTTGCCAGAATCTg                                      <  1:102991/40‑1 (MQ=38)                                                           aaTCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCGTGGcc                          <  1:978032/52‑1 (MQ=255)                                                           aaTCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCa       <  1:1459859/71‑1 (MQ=255)                                                           aaTCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCa       >  1:417316/1‑71 (MQ=255)                                                            aTCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTg                             <  1:1135080/48‑1 (MQ=255)                                                            aTCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACg          >  1:3021571/1‑67 (MQ=255)                                                            aTCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCAt      >  1:516616/1‑71 (MQ=255)                                                            aTCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCAt      >  1:1303410/1‑71 (MQ=255)                                                            aTCGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCAt      >  1:1092146/1‑71 (MQ=255)                                                              cGACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCg    >  1:335962/1‑71 (MQ=255)                                                               gACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCg    <  1:1403798/70‑1 (MQ=255)                                                               gACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCg    >  1:429505/1‑70 (MQ=255)                                                               gACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGc   >  1:2903989/1‑71 (MQ=255)                                                               gACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGc   <  1:2149195/71‑1 (MQ=255)                                                               gACAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGc   >  1:118379/1‑71 (MQ=255)                                                               gACAGTGTTATCAGAAAGATGGGTCGCCAGAATCTGCATGCCCTGGCCTTTGAGAGGAGCGa            <  1:2559795/62‑1 (MQ=255)                                                                aCAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGcc  <  1:645935/71‑1 (MQ=255)                                                                aCAGTGTTATCAGAAAGATCGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGcc  <  1:2358114/71‑1 (MQ=255)                                                                 cAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTg                             >  1:485096/1‑43 (MQ=255)                                                                 cAGTGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGcc  <  1:643210/70‑1 (MQ=255)                                                                    |                                                                   CCCGTTTTCTCCTGTCCCATCAAAATGCAGGTCGGGCGAGTGTAATCAATTTCGCGGAAATCGACAGCGTTATCAGAAAGATGGGTTGCCAGAATCTGCATGCCCTGGCCTTTGAGATGAGCGACGGCATCGCC  >  minE/2516589‑2516722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑