Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,605,272	A→G	L50L (CTT→CTC)	uspA ←	universal stress global response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,605,272	0	A	G	100.0%	251.4 / NA	70	L50L (CTT→CTC)	uspA	universal stress global response regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/47);  total (23/47)

CTCGGTCAGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAGGGG  >  minE/2605206‑2605338                                                                   |                                                                   cTCGGTCAGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGcc                                                                <  1:972821/71‑1 (MQ=255) cTCGGTCAGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGcc                                                                <  1:2825540/71‑1 (MQ=255)    ggTCAGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCgg                                                             >  1:2517018/1‑71 (MQ=255)    ggTCAGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCg                                                              <  1:1874631/70‑1 (MQ=255)    ggTCAGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCg                                                              <  1:333841/70‑1 (MQ=255)      tCAGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGgtg                                                           <  1:549538/71‑1 (MQ=255)        aGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGgtgt                                                          <  1:2851561/70‑1 (MQ=255)        aGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGgtgt                                                          >  1:669312/1‑70 (MQ=255)        aGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGta                                                         >  1:581670/1‑71 (MQ=255)            cATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAgg                                                     >  1:878158/1‑71 (MQ=255)            cATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAgg                                                     >  1:184921/1‑71 (MQ=255)                 tgtgTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCaga                                                <  1:351694/71‑1 (MQ=255)                   tgtCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCagag                                               <  1:3019727/70‑1 (MQ=255)                   tgtCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCagag                                               <  1:340750/70‑1 (MQ=255)                   tgtCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGagt                                              <  1:2890369/71‑1 (MQ=255)                         ttCAGAGATGCGTTTCTGCATATCACCCAGATTCACATGAATGAGCCCGGTGTATAGGTCAGAGTAGTTTa                                        >  1:2774653/1‑71 (MQ=255)                         ttCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGagtagt                                           <  1:255724/68‑1 (MQ=255)                         ttCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGtt                                          <  1:3030674/69‑1 (MQ=255)                         ttCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTa                                        >  1:310931/1‑71 (MQ=255)                         ttCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGGCAGagtagt                                           <  1:153409/68‑1 (MQ=255)                           cAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGttt                                         <  1:1023441/68‑1 (MQ=255)                           cAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGtt                                          <  1:2616287/67‑1 (MQ=255)                           cAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTa                                        <  1:2995018/69‑1 (MQ=255)                            agagATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACAt                                     >  1:2279874/1‑71 (MQ=255)                            agagATGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACAt                                     <  1:2363415/71‑1 (MQ=255)                                aTGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCa                                                  <  1:642939/54‑1 (MQ=255)                                aTGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCa                                                  <  1:1291498/54‑1 (MQ=255)                                 tGCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACg                                >  1:204969/1‑71 (MQ=255)                                  gCGTTTCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGt                               >  1:604764/1‑71 (MQ=255)                                     tttCTGCATATCACCCAGATTCACATCTATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGa                            <  1:217111/71‑1 (MQ=255)                                     tttCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGagt                                              <  1:52997/53‑1 (MQ=255)                                     tttCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTa                                        <  1:1704756/59‑1 (MQ=255)                                     tttCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTAc                                       <  1:1089165/60‑1 (MQ=255)                                       tCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTa                                        >  1:1849326/1‑57 (MQ=255)                                       tCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTg                              >  1:213830/1‑67 (MQ=255)                                       tCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGAt                           >  1:579957/1‑70 (MQ=255)                                       tCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGAt                           <  1:2630725/70‑1 (MQ=255)                                       tCTGCATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGAt                           <  1:2671812/70‑1 (MQ=255)                                           cATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCagag                      <  1:1946339/71‑1 (MQ=255)                                           cATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCa                         <  1:317921/68‑1 (MQ=255)                                           cATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCa                         >  1:972520/1‑68 (MQ=255)                                           cATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCa                         <  1:389435/68‑1 (MQ=255)                                           cATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCa                         <  1:1626826/68‑1 (MQ=255)                                           cATATCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCa                         <  1:2334183/68‑1 (MQ=255)                                            atatCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAgaga                     <  1:1576714/71‑1 (MQ=255)                                            atatCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAgaga                     <  1:526632/71‑1 (MQ=255)                                             tatCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTgg                             <  1:1728939/62‑1 (MQ=255)                                               tCACCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGaaa                   <  1:1929270/70‑1 (MQ=255)                                                 aCCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACtt                <  1:1196718/71‑1 (MQ=255)                                                 aCCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACt                 <  1:2171138/70‑1 (MQ=255)                                                 aCCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACt                 <  1:2649463/70‑1 (MQ=255)                                                 aCCCAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACt                 <  1:1486739/70‑1 (MQ=255)                                                   ccAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTa                                  <  1:320384/51‑1 (MQ=255)                                                   ccAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACttt               >  1:2229686/1‑70 (MQ=255)                                                   ccAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTc              <  1:2952127/71‑1 (MQ=255)                                                    cAGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCg             <  1:1249810/71‑1 (MQ=255)                                                     aGATTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCg             >  1:1414976/1‑70 (MQ=255)                                                       aTTCACATCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTa                                  <  1:2871954/47‑1 (MQ=255)                                                            caTCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTAc                                 <  1:893419/43‑1 (MQ=255)                                                            caTCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAg     <  1:2909975/71‑1 (MQ=255)                                                            caTCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAg     <  1:576461/71‑1 (MQ=255)                                                             aTCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTa                                        >  1:2607930/1‑35 (MQ=255)                                                             aTCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGt       <  1:2339077/68‑1 (MQ=255)                                                             aTCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAgg    >  1:726693/1‑71 (MQ=255)                                                             aTCAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAg     <  1:2206227/70‑1 (MQ=255)                                                               cAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAgggg  >  1:1960378/1‑71 (MQ=255)                                                               cAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAgggg  >  1:1553846/1‑71 (MQ=255)                                                               cAATGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAgggg  >  1:2341063/1‑71 (MQ=255)                                                                aaTGAGCCCGGTGTATAGGTCAGAGTAGTTTACAt                                     >  1:159704/1‑35 (MQ=255)                                                                aaTGAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAgggg  >  1:1235076/1‑70 (MQ=255)                                                                   |                                                                   CTCGGTCAGTGCATGATGTGTCTCTTCAGAGATGCGTTTCTGCATATCACCCAGATTCACATCAATAAGCCCGGTGTATAGGTCAGAGTAGTTTACATCTACGTGGATCAGAGAAACTTTCGCATTGTAGGGG  >  minE/2605206‑2605338

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑