| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,639,808 | A→G | T245A (ACG→GCG) *147Q (TAA→CAA) |
ugpQ → yhhA ← |
glycerophosphodiester phosphodiesterase, cytosolic conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,639,808 | 0 | A | G | 100.0% | 268.1 / NA | 75 | T245A (ACG→GCG) *147Q (TAA→CAA) | ugpQ yhhA | glycerophosphodiester phosphodiesterase, cytosolic conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (39/36); total (39/36) | |||||||||||
CAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCGT > minE/2639740‑2639873 | cagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCg > 1:2659390/1‑71 (MQ=255)cagAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCg > 1:3008712/1‑71 (MQ=255) agagTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCgg < 1:78075/71‑1 (MQ=255) gagTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGc > 1:1919425/1‑71 (MQ=255) gTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGcc > 1:936476/1‑70 (MQ=255) gctgcGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCaa > 1:424424/1‑70 (MQ=255) gctgcGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCaa < 1:1900269/70‑1 (MQ=255) ctgcGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATa < 1:657370/71‑1 (MQ=255) cgcTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGtt > 1:259850/1‑71 (MQ=255) cgcTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGtt > 1:505414/1‑71 (MQ=255) cTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGtttt > 1:2536317/1‑71 (MQ=255) gggTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCg > 1:2114974/1‑55 (MQ=255) gggTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTc < 1:2925719/69‑1 (MQ=255) gggTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCa < 1:104149/70‑1 (MQ=255) gggTGTGGATTGCATCTGTACCGACGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCaa < 1:1885567/71‑1 (MQ=255) gtgGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTCCAACgg < 1:395348/70‑1 (MQ=255) ggATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAAt > 1:524122/1‑71 (MQ=255) ggATTGCATCTGTACCGATGCGATTGACGTGATTGGTCAGAACTTTGCGGCCCAATAGTTTTCAACGGAAt > 1:1336645/1‑71 (MQ=255) gATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATg > 1:1753928/1‑71 (MQ=255) gATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATg < 1:2787598/71‑1 (MQ=255) aTTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACTGAATGt < 1:391434/71‑1 (MQ=255) aTTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGt < 1:2806839/71‑1 (MQ=255) aTTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGt < 1:2092011/71‑1 (MQ=255) ttGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGtttt > 1:1753297/1‑59 (MQ=255) gCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAg > 1:722914/1‑71 (MQ=255) aTCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGc < 1:50785/71‑1 (MQ=255) aTCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGc < 1:2155001/71‑1 (MQ=255) tCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAg < 1:2860589/50‑1 (MQ=255) tCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAg < 1:2203590/50‑1 (MQ=255) cTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAg < 1:93531/49‑1 (MQ=255) cTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCt > 1:115774/1‑70 (MQ=255) tGTACCGATGCGATTGACGTGATTGGTCGGAACTTTGCGGCCCAATAGtt < 1:1410328/50‑1 (MQ=255) gTACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGaa > 1:2840847/1‑59 (MQ=255) tACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTc > 1:2460527/1‑51 (MQ=255) tACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTgcg < 1:457015/70‑1 (MQ=255) tACCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGGTTTCAACGGAATGTCAGGCTgc < 1:1486216/69‑1 (MQ=255) aCCGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTgcggc > 1:1170940/1‑71 (MQ=255) cGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGt > 1:2387003/1‑71 (MQ=255) cGATGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGt < 1:1444159/71‑1 (MQ=255) aTGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACgg < 1:2429918/51‑1 (MQ=255) aTGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTc < 1:2967247/57‑1 (MQ=255) tGCGATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCt > 1:1653681/1‑71 (MQ=255) gATTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTg < 1:1665846/69‑1 (MQ=255) aTTGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGc > 1:948735/1‑71 (MQ=255) ttGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:1070928/1‑71 (MQ=255) tGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTg < 1:2403407/63‑1 (MQ=255) tGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTgg > 1:2154289/1‑67 (MQ=255) tGACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCt > 1:2067352/1‑70 (MQ=255) gACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCaa > 1:2039510/1‑40 (MQ=255) gACGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGa > 1:551427/1‑71 (MQ=255) aCGTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGCCTGa < 1:1878728/70‑1 (MQ=255) gTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTTCTGGGCTGAtt > 1:3019586/1‑70 (MQ=255) gTGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTc < 1:1413170/71‑1 (MQ=255) tGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTc < 1:407681/45‑1 (MQ=255) tGATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCa < 1:2724096/71‑1 (MQ=255) gATTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAg > 1:929849/1‑71 (MQ=255) aTTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCa > 1:2793536/1‑69 (MQ=255) aTTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAg < 1:579988/70‑1 (MQ=255) aTTGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGc < 1:2422553/71‑1 (MQ=255) tGGTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCAt > 1:320498/1‑71 (MQ=255) ggTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCAt < 1:1056191/70‑1 (MQ=255) ggTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCAt < 1:184746/70‑1 (MQ=255) gTCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATg < 1:514641/70‑1 (MQ=255) tCCGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTgcg > 1:324306/1‑46 (MQ=255) ccGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCg > 1:805732/1‑71 (MQ=255) cGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGc > 1:516019/1‑71 (MQ=255) cGAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGc > 1:1746941/1‑71 (MQ=255) gAACTTTGCGGCCCAATAGTTTTCAACGGAATGTCa < 1:852640/36‑1 (MQ=255) aaCTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTgaggc > 1:2058063/1‑44 (MQ=38) aaCTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCg > 1:2867108/1‑71 (MQ=255) aCTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGcc > 1:1205541/1‑69 (MQ=255) aCTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCGt > 1:620028/1‑71 (MQ=255) aCTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCGt > 1:768711/1‑71 (MQ=255) cTTTGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTg < 1:1447858/48‑1 (MQ=255) tttGCGGCCCAATAGTTTTCAACGGAATGTCAGGCTgc < 1:798498/38‑1 (MQ=255) | CAGAGTTGCTGCGCTGGGGTGTGGATTGCATCTGTACCGATGCGATTGACGTGATTGGTCCGAACTTTACGGCCCAATAGTTTTCAACGGAATGTCAGGCTGCGGCGTGCTGGGCTGATTCAGCATGTCGCCGT > minE/2639740‑2639873 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |