| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | minE | 2,681,918 | A→G | R598R (CGT→CGC) | yhgF ← | predicted transcriptional accessory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,681,918 | 0 | A | G | 96.7% | 206.2 / ‑1.6 | 60 | R598R (CGT→CGC) | yhgF | predicted transcriptional accessory protein |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (27/31); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.97e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
TTGATGATGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATGCGTTC > minE/2681853‑2681983 | ttGATGATGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAg > 1:1908149/1‑71 (MQ=255) gatgatGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGtt > 1:599123/1‑71 (MQ=255) atgatgTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAg > 1:2097140/1‑68 (MQ=255) atgTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCgc < 1:2883076/70‑1 (MQ=255) atgTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCgc < 1:1617682/70‑1 (MQ=255) tgTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCg > 1:1165873/1‑68 (MQ=255) gTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCgctgc > 1:2626190/1‑71 (MQ=255) ggTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGt < 1:929418/71‑1 (MQ=255) tCGGCACACCGAATTTCTCATCAGTAAAGTCAGCCGCTTTCAGGTTGAGCAg > 1:1001842/1‑52 (MQ=38) tCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAg > 1:1442826/1‑52 (MQ=255) ggCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATc > 1:371449/1‑69 (MQ=255) acCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCtt < 1:83202/71‑1 (MQ=255) ccGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCttt < 1:1308157/71‑1 (MQ=255) ccGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCttt < 1:1386050/71‑1 (MQ=255) ccGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCttt < 1:521834/71‑1 (MQ=255) cGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGt > 1:747388/1‑54 (MQ=255) cGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCttt > 1:2047090/1‑70 (MQ=255) cGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCttt > 1:2209382/1‑70 (MQ=255) gAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCttt > 1:216488/1‑69 (MQ=255) aaTTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTc < 1:465499/44‑1 (MQ=255) tttCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCgctg < 1:619000/46‑1 (MQ=255) ttCTCATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCgctg > 1:2356943/1‑45 (MQ=255) tctcATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCgctgct > 1:970054/1‑46 (MQ=255) tctcATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGc < 1:1252460/70‑1 (MQ=255) tctcATCAGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGc < 1:806582/70‑1 (MQ=255) catcaGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCa > 1:546346/1‑63 (MQ=255) atcaGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCgc > 1:579862/1‑38 (MQ=255) atcaGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCctgc < 1:2249546/70‑1 (MQ=255) atcaGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTCACCCATCAGATCTTTCAGTGCctgct > 1:496730/1‑71 (MQ=255) tcaGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCctgctg > 1:1906659/1‑71 (MQ=255) tcaGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCctgctg < 1:2681329/71‑1 (MQ=255) caGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCtgt > 1:2940550/1‑71 (MQ=255) aGTAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCtgt < 1:2581029/70‑1 (MQ=255) tAAAGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGtt < 1:1104745/71‑1 (MQ=255) aaaGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGt < 1:529921/38‑1 (MQ=255) aaGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgc > 1:210742/1‑71 (MQ=255) aaGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtg > 1:2958919/1‑70 (MQ=255) aGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCa > 1:2576798/1‑54 (MQ=255) aGTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgct < 1:2334720/71‑1 (MQ=255) gTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgct < 1:754572/70‑1 (MQ=255) gTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgct < 1:2543504/70‑1 (MQ=255) gTCAGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgct < 1:2464189/70‑1 (MQ=255) aGACGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTtgctgc > 1:2695942/1‑69 (MQ=255) aCGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc < 1:1972467/47‑1 (MQ=255) aCGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTc < 1:1968845/47‑1 (MQ=255) aCGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAg < 1:2138753/70‑1 (MQ=255) aCGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGa < 1:2115663/71‑1 (MQ=255) cGCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCatgctg > 1:2250766/1‑57 (MQ=255) cGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGaa < 1:90864/71‑1 (MQ=255) gCTTTCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAAt < 1:1710434/71‑1 (MQ=255) tttCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCctgctg < 1:2327832/54‑1 (MQ=255) tttCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGa < 1:796556/67‑1 (MQ=255) ttCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCttt < 1:735991/41‑1 (MQ=255) ttCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCttt < 1:1171702/41‑1 (MQ=255) ttCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATGCg > 1:2666966/1‑71 (MQ=255) ttCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATGCg > 1:107288/1‑71 (MQ=255) tCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATGCg < 1:2771338/70‑1 (MQ=255) tCAGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATGCg < 1:1847669/70‑1 (MQ=255) aGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCa > 1:297735/1‑40 (MQ=255) aGGTTGCGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATGCGTTc < 1:898017/71‑1 (MQ=255) | TTGATGATGTCGGTTACCGTCGGCACACCGAATTTCTCATCAGTAAAGTCAGACGCTTTCAGGTTACGCAGTTCGCTGCTGTTACCCATCAGATCTTTCAGTGCCTGCTGTGTTGCTGCCAGAATGCGTTC > minE/2681853‑2681983 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |