Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,703,672	G→A	A355T (GCC→ACC)	aroB →	3‑dehydroquinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,703,672	0	G	A	100.0%	181.9 / NA	60	A355T (GCC→ACC)	aroB	3‑dehydroquinate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (33/27);  total (33/27)

AATTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTATTAGC  >  minE/2703605‑2703739                                                                    |                                                                    aaTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACAcc                                                                   <  1:1316107/70‑1 (MQ=255)  aTTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCa                                                                  <  1:1079313/70‑1 (MQ=255)        ccGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCg                                                            <  1:871279/70‑1 (MQ=255)               cAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCaa                                                    >  1:636500/1‑71 (MQ=255)                  ttGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                                                           >  1:601830/1‑61 (MQ=255)                  ttGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAAt                                                   >  1:586824/1‑69 (MQ=255)                  ttGGTAAGAGTGAAATTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCa                                                 <  1:1295301/71‑1 (MQ=255)                   tGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAAt                                                   >  1:130891/1‑68 (MQ=255)                    ggTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGc                                               >  1:2534154/1‑71 (MQ=255)                     gTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAAt                                                   <  1:2336704/66‑1 (MQ=255)                        agagTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTg                                                        >  1:1715068/1‑58 (MQ=255)                        agagTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaa                                           >  1:63603/1‑71 (MQ=255)                        agagTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaa                                           >  1:5814/1‑71 (MQ=255)                          agTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                                                           <  1:1365839/53‑1 (MQ=255)                          agTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCg                                              <  1:2522854/66‑1 (MQ=255)                          agTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCg                                              <  1:612654/66‑1 (MQ=255)                          agTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCg                                              <  1:1533852/66‑1 (MQ=255)                          agTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCg                                              <  1:2143501/66‑1 (MQ=255)                            tGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaacaac                                       <  1:731657/71‑1 (MQ=255)                              aaGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTaacaac                                       >  1:620743/1‑69 (MQ=255)                                gTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa                                   <  1:729736/71‑1 (MQ=255)                                 ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                                                           >  1:739369/1‑46 (MQ=255)                                 ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGa                                                           >  1:151813/1‑46 (MQ=255)                                 ttCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGaa                                  <  1:1552917/71‑1 (MQ=255)                                      aGCGGCGTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTc                             <  1:1396499/71‑1 (MQ=255)                                       gcggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGt                              >  1:1207739/1‑69 (MQ=255)                                       gcggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCa                            <  1:1590340/71‑1 (MQ=255)                                       gcggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCa                            <  1:1534729/71‑1 (MQ=255)                                         ggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCg                                                            <  1:1463262/37‑1 (MQ=255)                                         ggcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCa                                                 <  1:2410696/48‑1 (MQ=255)                                          gcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCa                                                     <  1:2247999/43‑1 (MQ=255)                                          gcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGa                                   <  1:533485/61‑1 (MQ=255)                                          gcgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGc                         >  1:266782/1‑71 (MQ=255)                                           cgTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAgg                          >  1:1575280/1‑69 (MQ=255)                                            gTTTCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCg                       >  1:679304/1‑71 (MQ=255)                                               tCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     <  1:2577896/70‑1 (MQ=255)                                               tCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:2644986/1‑70 (MQ=255)                                               tCGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCt                     >  1:1700655/1‑70 (MQ=255)                                                cGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAgg                          >  1:924052/1‑64 (MQ=255)                                                cGCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAgg                          >  1:2476628/1‑64 (MQ=255)                                                 gCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGaa                                  <  1:2679139/55‑1 (MQ=255)                                                 gCACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTa                   <  1:865622/70‑1 (MQ=255)                                                  cACGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATc                 <  1:524727/71‑1 (MQ=255)                                                   aCGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATc                 >  1:2921492/1‑70 (MQ=255)                                                    cGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCa                            >  1:1189008/1‑58 (MQ=255)                                                    cGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCa                            >  1:1485083/1‑58 (MQ=255)                                                    cGAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATc                 >  1:764940/1‑69 (MQ=255)                                                     gAGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAg              <  1:743843/71‑1 (MQ=255)                                                      aGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGt                              <  1:820921/54‑1 (MQ=255)                                                      aGCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGc             >  1:332075/1‑71 (MQ=255)                                                       gCTTGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCg            >  1:1493460/1‑71 (MQ=255)                                                          tGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTc         >  1:863008/1‑71 (MQ=255)                                                          tGTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTc         >  1:2859632/1‑71 (MQ=255)                                                           gTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTc         >  1:2356439/1‑70 (MQ=255)                                                           gTTCTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTc         <  1:1400686/70‑1 (MQ=255)                                                              cTTAAGACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTAt      >  1:2146291/1‑70 (MQ=255)                                                              cTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTAt      >  1:1602726/1‑70 (MQ=255)                                                              cTTAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTAt      >  1:719387/1‑70 (MQ=255)                                                               ttAACACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCaa               >  1:209444/1‑60 (MQ=255)                                                                 aaCACCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTATTAGc  >  1:2343082/1‑71 (MQ=255)                                                                    |                                                                    AATTCTTCCGTTGGCAATTGGTAAGAGTGAAGTTCGCAGCGGCGTTTCGCACGAGCTTGTTCTTAACGCCATTGCCGATTGTCAATCAGCGTAACAACAAGAAAGGTCAGGCCGCTTATCAAGCGGTCTATTAGC  >  minE/2703605‑2703739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑