Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,726,174	G→A	R303R (CGC→CGT)	yheS ←	fused predicted transporter subunits and ATP‑binding components of ABC superfamily

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,726,174	0	G	A	100.0%	126.6 / NA	44	R303R (CGC→CGT)	yheS	fused predicted transporter subunits and ATP‑binding components of ABC superfamily
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/18);  total (26/18)

TGCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCATACGCTCGAGC  >  minE/2726107‑2726237                                                                    |                                                                tGCGATCGACATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGcgcacg                                                               >  1:1459225/1‑70 (MQ=255)  gCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACgg                                                              >  1:2388405/1‑70 (MQ=255)      tCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCt                                                         <  1:2880413/71‑1 (MQ=255)       cGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCt                                                         >  1:2915543/1‑70 (MQ=255)          cATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAg                                                     >  1:1675614/1‑71 (MQ=255)                ccGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAAc                                               >  1:2068049/1‑71 (MQ=255)                   cgcTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACggg                                            <  1:1276701/71‑1 (MQ=255)                    gcTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCgg                                                  <  1:683720/64‑1 (MQ=255)                    gcTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGt                                           >  1:1476312/1‑71 (MQ=255)                     cTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGtt                                          >  1:2739550/1‑71 (MQ=255)                        aCTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTc                                       >  1:1828451/1‑71 (MQ=255)                         cTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTc                                       >  1:1485527/1‑70 (MQ=255)                         cTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTc                                       >  1:2604666/1‑70 (MQ=255)                         cTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCg                                      <  1:1011147/71‑1 (MQ=255)                          tttttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGa                                     >  1:149038/1‑71 (MQ=255)                            tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGa                                     <  1:2838693/69‑1 (MQ=255)                            tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGa                                     >  1:2249124/1‑69 (MQ=255)                            tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGa                                     <  1:918301/69‑1 (MQ=255)                            tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGAc                                    <  1:154800/70‑1 (MQ=255)                            tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg                                   >  1:1840942/1‑71 (MQ=255)                            tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg                                   >  1:2566903/1‑71 (MQ=255)                            tttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACg                                   >  1:2960442/1‑71 (MQ=255)                             ttCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGATGTCGACGt                                  >  1:1955466/1‑71 (MQ=255)                                 aTCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTgcgc                              <  1:2151032/71‑1 (MQ=255)                                  tCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTgcgc                              <  1:2013384/70‑1 (MQ=255)                                      cAGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTgcg                               >  1:112529/1‑65 (MQ=255)                                       aGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCggggg                         >  1:692018/1‑70 (MQ=255)                                       aGTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCggggg                         >  1:1593412/1‑70 (MQ=255)                                        gTAACGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGc                        >  1:2726963/1‑70 (MQ=255)                                          aaCGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGaa                                                <  1:2578801/44‑1 (MQ=255)                                          aaCGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAAt                     >  1:877177/1‑71 (MQ=255)                                           aCGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTg                                 >  1:2665840/1‑58 (MQ=255)                                           aCGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAAt                     <  1:1278397/70‑1 (MQ=255)                                            cGGATTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAAt                     >  1:1734861/1‑69 (MQ=255)                                               aTTTGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCt                <  1:2434122/71‑1 (MQ=255)                                                 ttGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTc               >  1:1017058/1‑70 (MQ=255)                                                  tGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGa                                     >  1:2562679/1‑47 (MQ=255)                                                  tGGCAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTcc              <  1:485174/70‑1 (MQ=255)                                                     cAGGCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCg                                      <  1:149215/43‑1 (MQ=255)                                                       ggCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCATACGc        <  1:342429/71‑1 (MQ=255)                                                        gCTTTCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCATACGCt       >  1:1134171/1‑71 (MQ=255)                                                          tttCCGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCATACGCTc      >  1:2051768/1‑70 (MQ=255)                                                              cGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCggg                           <  1:2004961/45‑1 (MQ=255)                                                              cGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCATACGCTCGAGc  <  1:2717872/70‑1 (MQ=255)                                                              cGGCGCACGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCATACGCTCGAGc  <  1:543085/70‑1 (MQ=255)                                                                    |                                                                TGCGATCGCCATAGCCCGCGCTGACTTTTTCCATCTTCAGTAACGGATTTGGCAGGCTTTCCGGCGCGCGGAAGCTAAAGCGGAACGGGTTGTCGACGTGCGCGGGGGCAATTAGCTCCATACGCTCGAGC  >  minE/2726107‑2726237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑