Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	minE	2,802,519	T→A	D196V (GAT→GTT)	qor ←	quinone oxidoreductase, NADPH‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	minE	2,802,519	0	T	A	100.0%	155.6 / NA	54	D196V (GAT→GTT)	qor	quinone oxidoreductase, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (27/27);  total (27/27)

TGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGC  >  minE/2802451‑2802581                                                                     |                                                               tGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAA‑CACTCGACCAGAACct                                                             >  1:834588/1‑71 (MQ=255)        ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                       >  1:1290654/1‑71 (MQ=255)        ggAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCAc                                                       >  1:522034/1‑71 (MQ=255)          aaTCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGa                                                     <  1:2359961/71‑1 (MQ=255)          aaTCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGa                                                     >  1:2922496/1‑71 (MQ=255)           aTCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGAt                                                    <  1:285976/71‑1 (MQ=255)                   ccacGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaata                                            <  1:1683507/71‑1 (MQ=255)                    cacGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaataa                                           >  1:587306/1‑71 (MQ=255)                      cgcgCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAAc                                          >  1:181467/1‑70 (MQ=255)                         gcACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTg                                       <  1:2878467/70‑1 (MQ=255)                         gcACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGc                                      <  1:1074139/71‑1 (MQ=255)                         gcACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGc                                      <  1:1279053/71‑1 (MQ=255)                           aCTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCa                                    >  1:1228244/1‑71 (MQ=255)                           aCTTTCTTACCGCAGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaata                                            >  1:2360339/1‑63 (MQ=255)                             tttCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACg                                                      >  1:1788268/1‑51 (MQ=255)                             tttCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATa                                                   <  1:2985518/54‑1 (MQ=255)                             tttCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCa                                    >  1:715328/1‑69 (MQ=255)                             tttCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCAcg                                  >  1:2303293/1‑71 (MQ=255)                               tCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAAc                                          >  1:1796624/1‑61 (MQ=255)                                cTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaata                                            >  1:1548946/1‑58 (MQ=255)                                  tACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTc                                                         >  1:2915919/1‑43 (MQ=255)                                  tACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTc                                                         >  1:1620451/1‑43 (MQ=255)                                  tACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTc                                                         >  1:1477434/1‑43 (MQ=255)                                  tACCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGcc                              >  1:295123/1‑70 (MQ=255)                                   aCCGCCGGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGccc                             <  1:685770/70‑1 (MQ=255)                                      gccgGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCtt                         <  1:739870/71‑1 (MQ=255)                                       ccgGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCttt                        >  1:1206170/1‑71 (MQ=255)                                       ccgGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCttt                        <  1:1404865/71‑1 (MQ=255)                                        cgGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCtttt                       <  1:2354559/71‑1 (MQ=255)                                        cgGTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCtttt                       <  1:2642948/71‑1 (MQ=255)                                          gTGATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGc                           <  1:1364317/65‑1 (MQ=255)                                            gATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCt                          <  1:53025/64‑1 (MQ=255)                                            gATCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcg                   >  1:253877/1‑71 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGAt                                                    <  1:1938390/37‑1 (MQ=255)                                             aTCTCTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcgc                  >  1:1745628/1‑71 (MQ=255)                                              tctcTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGcc                                     <  1:3013856/51‑1 (MQ=255)                                              tctcTTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcgc                  >  1:1783513/1‑70 (MQ=255)                                              tctcTTTTAACCGCTCAACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcgcg                 >  1:2762021/1‑71 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGAt                                                    <  1:2017572/35‑1 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTtaataa                                           <  1:1080161/44‑1 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcgcgc                >  1:2765281/1‑71 (MQ=255)                                               ctctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAgcgcgc                >  1:1762641/1‑71 (MQ=255)                                                tctTTTAAACGCTCGACCAGAACCTCTTCACGATAGTtaata                                            <  1:1453576/42‑1 (MQ=38)                                                 ctTTTAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCt                                        >  1:2640615/1‑45 (MQ=255)                                                    ttAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCTCCCGCTTTTAGCGCGctct             <  1:655209/69‑1 (MQ=255)                                                    ttAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGctct             <  1:2751274/69‑1 (MQ=255)                                                    ttAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTgc           >  1:2234482/1‑71 (MQ=255)                                                    ttAACCGCTCGACCAGAACCTCTTCACGATAGTTAATAACATGCCAc                                   >  1:2445344/1‑47 (MQ=255)                                                       aCCGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCt        <  1:85187/71‑1 (MQ=255)                                                         cGCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCttt      <  1:1652730/71‑1 (MQ=255)                                                          gCTCGACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCttt      <  1:2541801/70‑1 (MQ=255)                                                              gACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTgc  <  1:500483/70‑1 (MQ=255)                                                              gACCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTg   <  1:2301534/69‑1 (MQ=255)                                                               aCCAGAACCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTa                      <  1:220161/49‑1 (MQ=255)                                                                     |                                                               TGCCCACGGAATCGTACACCACGCGCACTTTCTTACCGCCGGTGATCTCTTTTAACCGCTCGACCAGATCCTCTTCACGATAGTTAATAACCTGCCACGCGCCCGCTTTTAGCGCGCTCTGCGCTTTTTGC  >  minE/2802451‑2802581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑