| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,020,631 | Δ1 bp | 100% | coding (222/927 nt) | yqhG → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,020,631 | 0 | T | . | 86.1% | 115.8 / 16.1 | 36 | coding (222/927 nt) | yqhG | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base . (0/31); total (0/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.66e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTGG > minE/2020590‑2020675 | gcgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGttt < 1:1158543/67‑1 (MQ=255)gcgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGttt < 1:414784/67‑1 (MQ=255)gcgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGttt < 1:383912/67‑1 (MQ=255)gcgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGttt < 1:222152/67‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTAGGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:779235/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:1441637/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:770007/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:764616/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:711989/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:626396/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:526567/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:522358/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:492773/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:917918/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:1078772/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:929725/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:936344/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:2245375/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:1391994/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:2167292/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:2081712/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:1518341/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:1547100/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:1658888/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:1709031/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:1803454/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:1851795/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:1887502/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:1943354/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:19676/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:970002/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:2002269/63‑1 (MQ=255) cgcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:212052/63‑1 (MQ=255) gcTTACCCTGAACGACAATCTTACGCTGCCGGGCAATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:27237/62‑1 (MQ=255) cccTGAACGACAATCTTACGCTGCCGGGCTATAT‑‑‑‑TGGCTGGATTATGATCCGGTTtg < 1:83949/57‑1 (MQ=255) aTCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTgg < 1:1762915/67‑1 (MQ=255) ttGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTgg < 1:1420944/46‑1 (MQ=255) ttGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTgg < 1:971661/46‑1 (MQ=255) ttGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTgg < 1:899617/46‑1 (MQ=255) ttGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTgg < 1:819391/46‑1 (MQ=255) ttGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTgg < 1:456318/46‑1 (MQ=255) ttGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTgg < 1:118336/46‑1 (MQ=255) ttGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTgg < 1:1992954/46‑1 (MQ=255) ttGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTgg < 1:1931550/46‑1 (MQ=255) ttGGCTGGCTGGATTATGATCCGGATTGTGATTGTCAGGATTTTgg < 1:2305174/46‑1 (MQ=255) | GCGCTTACCCTGAACGACAATCTTACGCTGCCGGGCAATATTGGCTGGCTGGATTATGATCCGGTTTGTGATTGTCAGGATTTTGG > minE/2020590‑2020675 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |