| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 26,833 | Δ1 bp | 100% | coding (782/822 nt) | dapB → | dihydrodipicolinate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 26,833 | 0 | T | . | 100.0% | 11.5 / NA | 4 | coding (782/822 nt) | dapB | dihydrodipicolinate reductase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base . (4/0); total (4/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTG > minE/26789‑26856 | cgcgGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTC‑TTTTGATATGCGAGATGTACtt > 1:1999260/1‑66 (MQ=255)cgcgGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTC‑TTTTGATATGCGAGATGTACtt > 1:765244/1‑66 (MQ=255)cgcgGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTC‑TTTTGATATGCGAGATGTACTTg > 1:1474631/1‑67 (MQ=255)cgcgGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTC‑TTTTGATATGCGAGATGTACTTg > 1:1649/1‑67 (MQ=255) | CGCGGTAAGATCGGCTTTGTGGTTGAGTGGTAAGGAAAGCGGTCTTTTTGATATGCGAGATGTACTTG > minE/26789‑26856 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |