| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 873,160 | Δ1 bp | 100% | coding (352/975 nt) | cysB → | DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 873,160 | 0 | C | . | 88.9% | 23.7 / ‑1.0 | 9 | coding (352/975 nt) | cysB | DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base . (8/0); total (9/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGCC > minE/873134‑873200 | cAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGcc > 1:493158/1‑67 (MQ=255)cAAACGTCATCAAAGGCTTTATTGAG‑GTTATCCTc > 1:1268552/1‑35 (MQ=38)cAAACGTCATCAAAGGCTTTATTGAG‑GTTATCCTCGCGTTTCTTTGCa > 1:1205732/1‑48 (MQ=255)cAAACGTCATCAAAGGCTTTATTGAG‑GTTATCCTCGCGTTTCTTTGCa > 1:1237602/1‑48 (MQ=255)cAAACGTCATCAAAGGCTTTATTGAG‑GTTATCCTCGCGTTTCTTTGCa > 1:1661040/1‑48 (MQ=255)cAAACGTCATCAAAGGCTTTATTGAG‑GTTATCCTCGCGTTTCTTTGCa > 1:181734/1‑48 (MQ=255)cAAACGTCATCAAAGGCTTTATTGAG‑GTTATCCTCGCGTTTCTTTGCa > 1:2094487/1‑48 (MQ=255)cAAACGTCATCAAAGGCTTTATTGAG‑GTTATCCTCGCGTTTCTTTGCa > 1:576409/1‑48 (MQ=255)cAAACGTCATCAAAGGCTTTATTGAG‑GTTATCCTCGCGTTTCTTTGCa > 1:744112/1‑48 (MQ=255) | CAAACGTCATCAAAGGCTTTATTGAGCGTTATCCTCGCGTTTCTTTGCATATGCACCAGGGCTCGCC > minE/873134‑873200 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |