| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,015,455 | Δ1 bp | 100% | coding (614/1647 nt) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,015,455 | 0 | G | . | 100.0% | 93.2 / NA | 22 | coding (614/1647 nt) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base . (0/22); total (0/22) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTC > minE/1015429‑1015493 | catccAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:2281367/61‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:289757/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:989847/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:877185/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:820341/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:756979/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:705614/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:558756/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:515466/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:327076/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:298269/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:290294/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:273996/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:2300153/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:2037516/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:1751387/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:151278/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:1253038/64‑1 (MQ=255)cAACCAGGTAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:1208725/64‑1 (MQ=255)cAACCAGGTAA‑TTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:1110156/63‑1 (MQ=255) tAATTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:1996657/56‑1 (MQ=255) aTTTTTCAGTTTCCCC‑GCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc < 1:1545434/54‑1 (MQ=255) | CAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTC > minE/1015429‑1015493 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |