| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,524,377 | Δ1 bp | 100% | coding (996/1179 nt) | yfcJ ← | predicted transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,524,377 | 0 | G | . | 95.8% | 91.9 / ‑1.5 | 24 | coding (996/1179 nt) | yfcJ | predicted transporter |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (0/23); total (0/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
GAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCG > minE/1524320‑1524423 | gAGGGCGATATCCTGAAACGCGGCTTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:1786497/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:2196502/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:877462/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:802700/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:781041/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:664683/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:65735/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:618149/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:438970/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:415989/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:312073/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:2280210/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:1036792/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:1983141/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:1843362/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:1567330/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:1439644/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:1380274/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:1311308/67‑1 (MQ=255)gAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:118067/67‑1 (MQ=255) aGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:2195840/66‑1 (MQ=255) aGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:2059330/66‑1 (MQ=255) aGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGA‑GGGACGCGtt < 1:1508970/66‑1 (MQ=255) gCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCg < 1:573731/67‑1 (MQ=255) | GAGGGCGATATCCTGAAACGCGGCGTAACCGCCCAGTGCGGTGCCGCGAACTTGTGAGGGGACGCGTTTAACCACCTCCACGCCCAGCGCAGGAAAGATAAGCG > minE/1524320‑1524423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |