| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 300,036:1 | +TGT | 100% | coding (932/1782 nt) | mdlB → | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 300,036 | 1 | . | T | 98.1% | 179.5 / ‑3.2 | 53 | coding (932/1782 nt) | mdlB | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (52/0); total (53/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | minE | 300,036 | 2 | . | G | 98.1% | 179.4 / ‑3.1 | 53 | coding (932/1782 nt) | mdlB | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (52/0); total (53/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| * | minE | 300,036 | 3 | . | T | 98.1% | 180.0 / ‑3.2 | 53 | coding (932/1782 nt) | mdlB | fused predicted multidrug transporter subunits and ATP binding components of ABC superfamily |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base T (52/0); total (53/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGC‑‑‑TGTTGTTGCTGGTGAGCGCG > minE/299992‑300056 ||| acaaTTAATCGAACTGACCACGCAACAGGCGATGCTGCAACACGCTGTTGTTGTTGCTGGTGAgcgc > 1:1150025/4‑67 (MQ=255)aCCATTGATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:396037/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:562065/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:2147109/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:219071/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:2235403/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:2289532/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:246764/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:286719/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:319100/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:364334/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:377608/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:432696/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:43519/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:449409/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:2098236/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:638030/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:640292/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:713166/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:715026/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:756050/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:773339/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:815085/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:854628/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:873092/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:927666/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:930279/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1605965/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1031559/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1061585/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1156410/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1235342/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1242411/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1286380/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1336341/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1432695/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1451222/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1492320/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1005902/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1694567/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1710341/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:174214/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:175247/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1755084/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1822083/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1832609/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1892369/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1922565/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1953474/1‑67 (MQ=255)aCCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGC‑‑‑TGTTGTTGTTGCTg > 1:1404894/1‑59 (MQ=39)aCCATTAATCGAACTGACCACGCAACAGGCGA‑GCTGCAACAGGCTGTTGTTGTTGCTGGTGAGcgcg > 1:182613/1‑67 (MQ=255)aCCATTAATCGAACTGACCAAGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:1567430/1‑67 (MQ=255)aCCAGTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGCTGTTGTTGTTGCTGGTGAgcgc > 1:294364/1‑67 (MQ=255) ||| ACCATTAATCGAACTGACCACGCAACAGGCGATGCTGCAACAGGC‑‑‑TGTTGTTGCTGGTGAGCGCG > minE/299992‑300056 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |