| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,897,915:1 | +A | 100% | coding (747/1374 nt) | mpl → | UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,897,915 | 1 | . | A | 94.7% | 122.5 / 0.7 | 38 | coding (747/1374 nt) | mpl | UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase |
| Reads supporting (aligned to +/- strand): ref base . (0/2); new base A (0/36); total (0/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
| Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch. | |||||||||||
GGAGCGAGCAGGAGCTGGTGGGTGAGCAGGGTCACTGGCAGGCG‑AAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGC > minE/2897872‑2897955 | ggAGCGAGCAGGAGCTGGTGGGTGAGCAGGGTCACTGGCAGGCG‑AAAAAGCTGACCACCGATGCTTc < 1:1632396/67‑1 (MQ=255)ggAGCGAGCAGGAGCTGGTGGGTGAGCAGGGTCACTGGCAGGAG‑AAAAAGCTGACCACCGATGCTTc < 1:1940762/67‑1 (MQ=255) gggtgAGCAGGGTGACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:2243689/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:556608/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:2270709/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:2302903/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:326972/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:416403/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:426120/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:472414/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:509253/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:552569/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:2213807/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:611832/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:649031/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:763305/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:836160/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:859767/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:905314/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:954483/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:973208/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:1835551/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:1335170/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:1373002/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:1461813/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:1462239/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:152082/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:1710327/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:1778931/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:1811443/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:1022229/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:1921931/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:1972711/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:2047717/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:2059765/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:2156856/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:219510/66‑1 (MQ=255) gggtgAGCAGGGTCACTGGCAGGCGAAAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGc < 1:2199717/66‑1 (MQ=255) | GGAGCGAGCAGGAGCTGGTGGGTGAGCAGGGTCACTGGCAGGCG‑AAAAAGCTGACCACCGATGCTTCCGAATGGGAAGTTTTGC > minE/2897872‑2897955 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |