| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 446,205:1 | +C | 100% | coding (1511/2685 nt) | kdpD ← | fused sensory histidine kinase in two‑component regulatory system with KdpE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 446,205 | 1 | . | C | 100.0% | 14.6 / NA | 6 | V504G (GTA→GGA) | kdpD | fused sensory histidine kinase in two‑component regulatory system with KdpE |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base C (6/0); total (6/0) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GTGTGCGTTGCTCGCGGTAACGGGCTA‑CCCGCGCCTGATAACGCACGCCAGCAGTAAGGTTCCCG > minE/446179‑446243 | gtgtGCGTTGCTCGCGGTAACGGGCTACCCCGCGCCTGATACCGCCCGCCAGCAGTAAGGTTCCCg > 1:1542300/1‑66 (MQ=255)gtgtGCGTTGCTCGCGGTAACGGGCTACCCCGCGCCTGATAACGCCCGCCAGCAGTAAGGTTCCCg > 1:838890/1‑66 (MQ=255)gtgtGCGTTGCTCGCGGTAACGGGCTACCCCGCGCCTGATAACGCACGCCAGCAGTAAGGTTCCCg > 1:1534355/1‑66 (MQ=255)gtgtGCGTTGCTCGCGGTAACGGGCTACCCCGCGCCTGATAACGCACGCCAGCAGTAAGGTTCCCg > 1:1712635/1‑66 (MQ=255)gtgtGCGTTGCTCGCGGTAACGGGCTACCCCGCGCCTGATAACGCACGCCAGCAGTAAGGTTCCCg > 1:1759989/1‑66 (MQ=255)gtgtGCGTTGCTCGCGGTAACGGGCTACCCCGCGCCTGATAACGCACGCCAGCAGTAAGGTTCCCg > 1:652111/1‑66 (MQ=255) | GTGTGCGTTGCTCGCGGTAACGGGCTA‑CCCGCGCCTGATAACGCACGCCAGCAGTAAGGTTCCCG > minE/446179‑446243 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |